Coupling termination of transcription to messenger RNA maturation in yeast

Science

Volumn 280, Issue 5361, 1998, Pages 298-301

  Birse, Charles E ^a,c   Minvielle Sebastia, Lionel ^b   Lee, Barbara A ^a   Keller, Walter ^b   Proudfoot, Nick J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF BASEL   (Switzerland)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; MESSENGER RNA PRECURSOR; POLYADENYLATED RNA;

ARTICLE; GENE EXPRESSION REGULATION; NONHUMAN; POLYADENYLATION; PRIORITY JOURNAL; RNA BINDING; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION REGULATION; TRANSCRIPTION TERMINATION; YEAST;

CYTOCHROME C GROUP; CYTOCHROMES C; FUNGAL PROTEINS; GENES, FUNGAL; MRNA CLEAVAGE AND POLYADENYLATION FACTORS; MUTATION; NUCLEAR PROTEINS; POLY A; RNA POLYMERASE II; RNA PRECURSORS; RNA, FUNGAL; RNA, MESSENGER; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TEMPERATURE; TRANSCRIPTION, GENETIC;

SACCHAROMYCES CEREVISIAE;

EID: 0032502825     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5361.298     Document Type: Article

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37. 35S]methionine. After incubation for 10 min at 45°C, they were assayed for their ability to bind to agarose-bound poly(U) for 15 min, as described [M. S. Swanson and G. Dreyfuss, Mol. Cell. Biol. 8, 2237 (1988)]. After extensive washes, the bound material was released by boiling with SDS-polyacrylamide gel electrophoresis (PAGE) loading buffer and loaded on 10% denaturing protein gels. After electrophoresis, the gels were fixed, dried, and subjected to autoradiography.
38. We thank S. Barabino, S. Butler, F. Lacroute, A. Sachs, F. Sherman, and R. Young for various yeast strains, P. J. Preker for the pap1 mutants, and J. Mellor for plasmids. We thank D. Pritlove and members of the N.J.P. lab for critical reading of the manuscript and for their help and advice. Supported by a Wellcome Programme Grant (032773) to N.J.P. and grants by the Kantons of Basel, the Swiss National Science Foundation, and the European Union (through the Bundesamt für Bildung und Wissenschaft, Bern) to W.K.