Analytical methods for the characterization of cationic lipid-nucleic acid complexes

Human Gene Therapy

Volumn 9, Issue 3, 1998, Pages 341-351

  Ferrari, Marilyn E ^a   Nguyen, Cuong M ^a   Zelphati, Olivier ^a   Tsai, Yali ^a   Felgner, Philip L ^a  

^a VICAL INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATION; DEOXYRIBONUCLEASE I; DETERGENT; FLUORESCAMINE; LIPID; NUCLEASE; NUCLEIC ACID; POLYCARBONATE;

ANALYTIC METHOD; ARTICLE; COMPLEX FORMATION; DNA BINDING; FILTRATION; MOLECULAR STABILITY;

EID: 0032501957     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.1998.9.3-341     Document Type: Article

References (6)

1. AUSUBEL, F.M., BRENT, R., KINGSTON, R.E., MOORE, D.D., SEIDMAN, J.G., SMITH, J.A., and STRUHL, K. (1987). Current Protocols in Molecular Biology. Vol. 2 (Green Publishing Assoc., New York).
2. BEHR, J.P., DEMENEIX, B., LOEFFLER, J.P., and PEREZ-MUTUL, J. (1989). Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA. Proc. Natl. Acad. Sci. USA 86, 6982-6986.
3. BERTLING, W.M., GAREIS, M., PASPALEEVA, V., ZIMMER, A., KREUTER, J., NURNBERG, E., and HARRER, P. (1991). Use of liposomes, viral capsids, and nanoparticles as DNA carriers. Biotechnol. Appl. Biochem. 13, 390-405.
4. BRIGHAM, K.L., MEYRICK, B., CHRISTMAN, B., MAGNUSON, M., KING, G., and BERRY, L.C., JR. (1989). In vivo transfection of murine lungs with a functioning prokaryotic gene using a liposome vehicle. Am. J. Med. Sci. 298, 278-281.
5. CAPLEN, N.J., ALTON, E.W., MIDDLETON, P.G., DORIN, J.R., STEVENSON, B.J., GAO, X., DURHAM, S.R., JEFFERY, P.K., HODSON, M.E., COUTELLE, C., HUANG, L., PORTEOUS, D.J., WILLIAMSON, R., and GEDDES, D.M. (1995). Liposome-mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis [published erratum appears in Nature Med. 1, 272, 1995]. Nature Med. 1, 39-46.
6. ZELPHATI, O., NGUYEN, C., FERRARI, M., FELGNER, J., TSAI, Y., and FELGNER, P. (1998). New stable and monodisperse lipoplex formulations for gene delivery. Gene Ther. (submitted).