Consensus phosphorylation sites of human GABA(C)/GABA(ρ) receptors are not critical for inhibition by protein kinase C activation

Neuroscience Letters

Volumn 255, Issue 1, 1998, Pages 17-20

  Kusama, Tadashi ^a   Hatama, Kouji ^a   Sakurai, Manabu ^a   Kizawa, Yasuo ^a   Uhl, George R ^b   Murakami, Hajime ^a  

^a NIHON UNIVERSITY   (Japan)

^b Molecular Neurobiology   (United States)

Author keywords

1 subunit; 2 subunit; GABA(C) receptor; Mutagenesis; Phosphorylation; Protein kinase C; Voltage clamp; Xenopus oocyte

Indexed keywords

4 AMINOBUTYRIC ACID RECEPTOR; CHLORIDE CHANNEL; ENZYME ACTIVATOR; PHORBOL 13 ACETATE 12 MYRISTATE; PROTEIN KINASE C;

ANIMAL CELL; ARTICLE; BETA CHAIN; DRUG RECEPTOR BINDING; ENDOCYTOSIS; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; PRIORITY JOURNAL; RECEPTOR UPREGULATION; SITE DIRECTED MUTAGENESIS; XENOPUS;

ANIMALS; CONSENSUS SEQUENCE; ELECTRIC CONDUCTIVITY; ENZYME ACTIVATION; FEMALE; HUMANS; MUTAGENESIS, SITE-DIRECTED; OOCYTES; PATCH-CLAMP TECHNIQUES; PHOSPHORYLATION; PROTEIN KINASE C; RECEPTORS, GABA; TETRADECANOYLPHORBOL ACETATE; XENOPUS LAEVIS;

EID: 0032500785     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(98)00696-X     Document Type: Article

References (20)

1. Conradt M., Stoffel W. Inhibition of the high-affinity grain glutamate transporter GLAST-1 via direct phosphorylation. J. Neurochem. 68:1997;1244-1251.
2. Cutting G.R., Lu L., O'Hara B.F., Kasch L.M., Rafizadeh C.M., Donovan D.M., Shimada S., Antonarakis S.E., Guggino W.B., Uhl G.R., Kazazian H.H. Cloning of the γ-aminobutyric acid (GABA) ρ1 cDNA: a GABA receptor subunit highly expressed in the retina. Proc. Natl. Acad. Sci. USA. 88:1991;2673-2677.
3. Cutting G.R., Curristin S., Zoghbi H., O'Hara B., Seldin M.F., Uhl G.R. Identification of a putative γ-aminobutyric acid (GABA) receptor subunit rho2 cDNA and colocalization of the genes encoding rho2 (GABRR2) and rho1 (GABRR1) to human chromosome 6q14-q21 and mouse chromosome 4. Genomics. 12:1992;801-806.
4. Fagerstrom S., Pahlman S., Nanberg E. Protein kinase C-dependent tyrosine phosphorylation of p130cas in differentiating neuroblastoma cells. J. Biol. Chem. 273:1998;2336-2343.
5. C receptors on rat retinal bipolar cells. Eur. J. Pharmacol. 288:1994;97-104.
6. +/glucose cotransporter expression by protein kinases in Xenopus laevis oocytes. J. Biol. Chem. 271:1996;14740-14746.
7. c Receptors, Humana Press, 1997, pp. 297-323.
8. Kellenberger S., Malherbe P., Sigel E. Function of the α1β2γ2S γ-aminobutyric acid type A receptor is regulated by protein kinase C via multiple phosphorylation sites. J. Biol. Chem. 267:1992;25660-25663.
9. A receptor function by protein kinase C phosphorylation. Neuron. 12:1994;1081-1095.
10. Kusama T., Wang T.-L., Guggino W.B., Cutting G.R., Uhl G.R. GABAρ2 receptor pharmacological profile: GABA recognition site similarities to ρ1. Eur. J. Pharmacol. 245:1993;83-84.
11. Kusama T., Spivak C.E., Whiting P., Dawson V.L., Schaeffer J.C., Uhl G.R. Pharmacology of GABA ρ1 and GABAα/β receptors expressed in Xenopus oocytes and COS cells. Br. J. Pharmacol. 109:1993;200-206.
12. Kusama T., Sakurai M., Kizawa Y., Uhl G.R., Murakami H. GABAρ1 receptor: inhibition by protein kinase C activators. Eur. J. Pharmacol. 291:1995;431-434.
13. Leidenheimer N.J., McQuilkin S.J., Hahner L.D., Whiting P., Harris R.A. Activation of protein kinase C selectively inhibits the γ-aminobutyric acid A receptor: role of desensitization. Mol. Pharmacol. 41:1992;1116-1123.
14. 2+/calmodulin-dependent protein kinase. J. Biol. Chem. 267:1992;12197-12201.
15. Pearson R.B., Kemp B.E. Protein kinase phosphorylation site sequences and consensus specificity motifs: tabulations. Methods Enzymol. 200:1991;62-81.
16. C receptors on hybrid bass retinal bipolar cells. J. Neurophysiol. 74:1995;1920-1928.
17. 1 receptor RNA induces bicuculline-, barbiturate-, and benzodiazepine-insensitive γ-aminobutyric acid responses in Xenopus oocytes. Mol. Pharmacol. 41:1992;683-687.
18. Uchiyama M., Hirai K., Hishinuma F., Akagi H. Down-regulation of glycine receptor channels by protein kinase C in Xenopus oocytes injected with synthetic RNA. Mol. Brain Res. 24:1994;295-300.
19. +-ATPase from mammalian kidneys and Xenopus oocytes by protein kinases: characterization of the phosphorylation site for protein kinase C. Cell. Physiol. Biochem. 7:1997;1-18.
20. Yanish-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:1985;103-119.