Isolation of acidophilic methane-oxidizing bacteria from Northern Peat Wetlands

Science

Volumn 282, Issue 5387, 1998, Pages 281-284

  Dedysh, Svetlana N ^a,b   Panikov, Nicolai S ^a   Liesack, Werner ^c   Großkopf, Regine ^c   Zhou, Jizhong ^b   Tiedje, James M ^b  

^a RUSSIAN ACADEMY OF SCIENCES   (Russian Federation)

^b MICHIGAN STATE UNIVERSITY   (United States)

^c MAX PLANCK INSTITUTE FOR TERRESTRIAL MICROBIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOGEOCHEMICAL CYCLE; METHANE; PEAT; SOIL MICROORGANISM; WETLAND;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIUM ISOLATION; GENE SEQUENCE; METHYLOCOCCACEAE; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; DNA, BACTERIAL; DNA, RIBOSOMAL; EVOLUTION; GENES, BACTERIAL; GRAM-NEGATIVE AEROBIC RODS AND COCCI; HYDROGEN-ION CONCENTRATION; METHANE; METHYLOCOCCACEAE; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; OXYGENASES; PHYLOGENY; RNA, RIBOSOMAL, 16S; RUSSIA; SEQUENCE ALIGNMENT; SIBERIA; SOIL MICROBIOLOGY; SOLUBILITY;

BACTERIA (MICROORGANISMS); BEIJERINCKIA; BEIJERINCKIA INDICA SUBSP. INDICA; METHYLOCOCCACEAE;

EID: 0032500735     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5387.281     Document Type: Article

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19. 4-C.
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21. _, ibid., p. 569.
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29. PCR-mediated amplification of the 16S rRNA genes and sequence analyses were done as described [W. Liesack, and K. Finster, Int. J. Syst. Bacteriol. 44, 753 (1994)]. The nucleotide sequence of the 16S rRNA gene of strain K has been deposited in the EMBL (European Molecular Biology Laboratory), GenBank, and DDBJ (DNA Data Bank of Japan) databases under accession number Y17144. The phylogenetic position of strains S6, K, and M131 was determined by comparing their 16S rRNA gene sequence with α-proteobacterial reference sequences for which at least 1000 nucleotide sequence positions were available. Trees were constructed for a sequence stretch ranging from positions 28 to 1477(Escherichia coli 16S rRNA numbering) by using distance matrix and maximum-likelihood methods and the ARB program package [J. Felsenstein, PHYLIP (phylogeny inference package), version 3.5c (University of Washington, Seattle, WA, 1993); B. L. Maidak et al., Nucleic Acids Res. 24, 82 (1996); O. Strunk and W. Ludwig, ARB: A Software Environment for Sequence Data (Technische Univ., Munich, Germany, 1996)].
30. PCR-mediated amplification of the 16S rRNA genes and sequence analyses were done as described [W. Liesack, and K. Finster, Int. J. Syst. Bacteriol. 44, 753 (1994)]. The nucleotide sequence of the 16S rRNA gene of strain K has been deposited in the EMBL (European Molecular Biology Laboratory), GenBank, and DDBJ (DNA Data Bank of Japan) databases under accession number Y17144. The phylogenetic position of strains S6, K, and M131 was determined by comparing their 16S rRNA gene sequence with α-proteobacterial reference sequences for which at least 1000 nucleotide sequence positions were available. Trees were constructed for a sequence stretch ranging from positions 28 to 1477(Escherichia coli 16S rRNA numbering) by using distance matrix and maximum-likelihood methods and the ARB program package [J. Felsenstein, PHYLIP (phylogeny inference package), version 3.5c (University of Washington, Seattle, WA, 1993); B. L. Maidak et al., Nucleic Acids Res. 24, 82 (1996); O. Strunk and W. Ludwig, ARB: A Software Environment for Sequence Data (Technische Univ., Munich, Germany, 1996)].
31. PCR-mediated amplification of the 16S rRNA genes and sequence analyses were done as described [W. Liesack, and K. Finster, Int. J. Syst. Bacteriol. 44, 753 (1994)]. The nucleotide sequence of the 16S rRNA gene of strain K has been deposited in the EMBL (European Molecular Biology Laboratory), GenBank, and DDBJ (DNA Data Bank of Japan) databases under accession number Y17144. The phylogenetic position of strains S6, K, and M131 was determined by comparing their 16S rRNA gene sequence with α-proteobacterial reference sequences for which at least 1000 nucleotide sequence positions were available. Trees were constructed for a sequence stretch ranging from positions 28 to 1477(Escherichia coli 16S rRNA numbering) by using distance matrix and maximum-likelihood methods and the ARB program package [J. Felsenstein, PHYLIP (phylogeny inference package), version 3.5c (University of Washington, Seattle, WA, 1993); B. L. Maidak et al., Nucleic Acids Res. 24, 82 (1996); O. Strunk and W. Ludwig, ARB: A Software Environment for Sequence Data (Technische Univ., Munich, Germany, 1996)].
32. PCR-mediated amplification of the 16S rRNA genes and sequence analyses were done as described [W. Liesack, and K. Finster, Int. J. Syst. Bacteriol. 44, 753 (1994)]. The nucleotide sequence of the 16S rRNA gene of strain K has been deposited in the EMBL (European Molecular Biology Laboratory), GenBank, and DDBJ (DNA Data Bank of Japan) databases under accession number Y17144. The phylogenetic position of strains S6, K, and M131 was determined by comparing their 16S rRNA gene sequence with α-proteobacterial reference sequences for which at least 1000 nucleotide sequence positions were available. Trees were constructed for a sequence stretch ranging from positions 28 to 1477(Escherichia coli 16S rRNA numbering) by using distance matrix and maximum-likelihood methods and the ARB program package [J. Felsenstein, PHYLIP (phylogeny inference package), version 3.5c (University of Washington, Seattle, WA, 1993); B. L. Maidak et al., Nucleic Acids Res. 24, 82 (1996); O. Strunk and W. Ludwig, ARB: A Software Environment for Sequence Data (Technische Univ., Munich, Germany, 1996)].
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36. Reference strain of B. indica was obtained from ATCC (accession number 9039).
37. We thank C. Flegler (Michigan State University Electron Microscope Center) for assistance with electron microscopy. Supported in part by the Russian Fund of Fundamental Research (grant 96-04-49321), by NSF (grants INT9315089 for Russian collaborative work and DEB9120006), and by the European Community RTD Programme in Biotechnology (grant EV5V-CT94-0499).