High-efficiency incorporation in vivo of tyrosine analogues with altered hydroxyl acidity in place of the catalytic tyrosine-14 of Δ5-3-ketosteroid isomerase of Comamonas (Pseudomonas) testosteroni: Effects of the modifications on isomerase kinetics

Biochemistry

Volumn 37, Issue 27, 1998, Pages 9738-9742

  Brooks, Bob ^a   Phillips, Robert S ^b   Benisek, William F ^a,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3BETA HYDROXY DELTA5 STEROID DEHYDROGENASE; AMINO ACID; BACTERIAL ENZYME; TYROSINE; TYROSINE DERIVATIVE;

ARTICLE; COMAMONAS TESTOSTERONI; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME MODIFICATION; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SUBSTITUTION; CATALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORINE RADIOISOTOPES; GRAM-NEGATIVE AEROBIC RODS AND COCCI; HYDROGEN-ION CONCENTRATION; HYDROXIDES; KINETICS; MASS SPECTROMETRY; PHENYLALANINE; STEROID ISOMERASES; TYROSINE;

BACTERIA (MICROORGANISMS); COMAMONAS TESTOSTERONI; ESCHERICHIA COLI; PSEUDOMONAS;

EID: 0032493478     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi980454x     Document Type: Article

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