Auxin-dependent cell expansion mediated by overexpressed auxin-binding protein 1

Science

Volumn 282, Issue 5391, 1998, Pages 1114-1117

  Jones, Alan M ^a   Im, Kyung Hoam ^a   Savka, Michael A ^b,d   Wu, Ming Jing ^a   DeWitt, N Gregory ^a   Shillito, Raymond ^c   Binns, Andrew N ^b  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c AgrEvo Research Center   (United States)

^d UNIVERSITY OF WEST FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUXIN; HORMONE BINDING PROTEIN;

ARABIDOPSIS; ARTICLE; CELL GROWTH; CELL SIZE; HORMONAL REGULATION; MAIZE; NONHUMAN; PLANT CELL; PLANT GROWTH; PRIORITY JOURNAL;

CELL LINE; CELL SIZE; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; INDOLEACETIC ACIDS; PHENOTYPE; PLANT GROWTH REGULATORS; PLANT LEAVES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; RECEPTORS, CELL SURFACE; TETRACYCLINES; TOBACCO; TRANSFORMATION, GENETIC; TRANSGENES; ZEA MAYS;

ARABIDOPSIS; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; ZEA MAYS;

EID: 0032491447     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. A. M. Jones, Annu. Rev. Plant Physiol. Plant Mol. Biol. 45, 393 (1994); R. M. Napier, J. Exp. Bot. 46, 1787 (1995).
2. A. M. Jones, Annu. Rev. Plant Physiol. Plant Mol. Biol. 45, 393 (1994); R. M. Napier, J. Exp. Bot. 46, 1787 (1995).
3. S. Abel and A. Theologis, Plant Physiol. 111, 9 (1996); P. M. Ray, Curr. Top. Plant Biochem. Physiol. 11, 18 (1992); U. Kutschera and W. R. Briggs, Proc. Natl. Acad. Sci. U.S.A. 84, 2747 (1987).
4. S. Abel and A. Theologis, Plant Physiol. 111, 9 (1996); P. M. Ray, Curr. Top. Plant Biochem. Physiol. 11, 18 (1992); U. Kutschera and W. R. Briggs, Proc. Natl. Acad. Sci. U.S.A. 84, 2747 (1987).
5. S. Abel and A. Theologis, Plant Physiol. 111, 9 (1996); P. M. Ray, Curr. Top. Plant Biochem. Physiol. 11, 18 (1992); U. Kutschera and W. R. Briggs, Proc. Natl. Acad. Sci. U.S.A. 84, 2747 (1987).
6. J. C. Brown and A. M. Jones, J. Biol. Chem. 269, 21136 (1994); M. D. Edgerton, A. Tropsha, A. M. Jones, Phytochemistry 35, 1111 (1994); P. M. Ray, U. Dohrmann, R. Hertel, Plant Physiol. 60, 585 (1977); S. Shimomura, S. Sotobayashi, M. Futai, T. Fukui, J. Biochem. 99, 1513 (1986); A. M. Jones and M. A. Venis, Proc. Natl. Acad. Sci. U.S.A. 86, 6153 (1989).
7. J. C. Brown and A. M. Jones, J. Biol. Chem. 269, 21136 (1994); M. D. Edgerton, A. Tropsha, A. M. Jones, Phytochemistry 35, 1111 (1994); P. M. Ray, U. Dohrmann, R. Hertel, Plant Physiol. 60, 585 (1977); S. Shimomura, S. Sotobayashi, M. Futai, T. Fukui, J. Biochem. 99, 1513 (1986); A. M. Jones and M. A. Venis, Proc. Natl. Acad. Sci. U.S.A. 86, 6153 (1989).
8. J. C. Brown and A. M. Jones, J. Biol. Chem. 269, 21136 (1994); M. D. Edgerton, A. Tropsha, A. M. Jones, Phytochemistry 35, 1111 (1994); P. M. Ray, U. Dohrmann, R. Hertel, Plant Physiol. 60, 585 (1977); S. Shimomura, S. Sotobayashi, M. Futai, T. Fukui, J. Biochem. 99, 1513 (1986); A. M. Jones and M. A. Venis, Proc. Natl. Acad. Sci. U.S.A. 86, 6153 (1989).
9. J. C. Brown and A. M. Jones, J. Biol. Chem. 269, 21136 (1994); M. D. Edgerton, A. Tropsha, A. M. Jones, Phytochemistry 35, 1111 (1994); P. M. Ray, U. Dohrmann, R. Hertel, Plant Physiol. 60, 585 (1977); S. Shimomura, S. Sotobayashi, M. Futai, T. Fukui, J. Biochem. 99, 1513 (1986); A. M. Jones and M. A. Venis, Proc. Natl. Acad. Sci. U.S.A. 86, 6153 (1989).
10. J. C. Brown and A. M. Jones, J. Biol. Chem. 269, 21136 (1994); M. D. Edgerton, A. Tropsha, A. M. Jones, Phytochemistry 35, 1111 (1994); P. M. Ray, U. Dohrmann, R. Hertel, Plant Physiol. 60, 585 (1977); S. Shimomura, S. Sotobayashi, M. Futai, T. Fukui, J. Biochem. 99, 1513 (1986); A. M. Jones and M. A. Venis, Proc. Natl. Acad. Sci. U.S.A. 86, 6153 (1989).
11. D. Harnden and A. M. Jones J. Plant Growth Reg. 14, 109 (1995).
12. A. M. Jones and E. Herman, Plant Physiol. 101, 595 (1993); W. Diekmann, M. A. Venis, D. C. Robinson, Proc. Natl. Acad. Sci. U.S.A. 92, 3425 (1995); H. Barbier-Brygoo et al., Plant J. 1, 83 (1991); A. Ruck, K. Palme, M. A. Venis, R. Napier, H. Felle, ibid. 4, 41 (1993); M. R. Blatt and G. Thiel, Annu. Rev. Plant Physiol. Plant Mol. Biol. 44, 543 (1994).
13. A. M. Jones and E. Herman, Plant Physiol. 101, 595 (1993); W. Diekmann, M. A. Venis, D. C. Robinson, Proc. Natl. Acad. Sci. U.S.A. 92, 3425 (1995); H. Barbier-Brygoo et al., Plant J. 1, 83 (1991); A. Ruck, K. Palme, M. A. Venis, R. Napier, H. Felle, ibid. 4, 41 (1993); M. R. Blatt and G. Thiel, Annu. Rev. Plant Physiol. Plant Mol. Biol. 44, 543 (1994).
14. A. M. Jones and E. Herman, Plant Physiol. 101, 595 (1993); W. Diekmann, M. A. Venis, D. C. Robinson, Proc. Natl. Acad. Sci. U.S.A. 92, 3425 (1995); H. Barbier-Brygoo et al., Plant J. 1, 83 (1991); A. Ruck, K. Palme, M. A. Venis, R. Napier, H. Felle, ibid. 4, 41 (1993); M. R. Blatt and G. Thiel, Annu. Rev. Plant Physiol. Plant Mol. Biol. 44, 543 (1994).
15. A. M. Jones and E. Herman, Plant Physiol. 101, 595 (1993); W. Diekmann, M. A. Venis, D. C. Robinson, Proc. Natl. Acad. Sci. U.S.A. 92, 3425 (1995); H. Barbier-Brygoo et al., Plant J. 1, 83 (1991); A. Ruck, K. Palme, M. A. Venis, R. Napier, H. Felle, ibid. 4, 41 (1993); M. R. Blatt and G. Thiel, Annu. Rev. Plant Physiol. Plant Mol. Biol. 44, 543 (1994).
16. A. M. Jones and E. Herman, Plant Physiol. 101, 595 (1993); W. Diekmann, M. A. Venis, D. C. Robinson, Proc. Natl. Acad. Sci. U.S.A. 92, 3425 (1995); H. Barbier-Brygoo et al., Plant J. 1, 83 (1991); A. Ruck, K. Palme, M. A. Venis, R. Napier, H. Felle, ibid. 4, 41 (1993); M. R. Blatt and G. Thiel, Annu. Rev. Plant Physiol. Plant Mol. Biol. 44, 543 (1994).
17. R transcript and was shown to be homozygous for this transgene, was used for ABP1 transformations. T-DNA vectors pMJ10 (ABP1 gene construct in pBin-HYGO-TX) and vector-only plasmid, pBin-HYGO-TX, were used to transform R7 using standard Agrobacterium-mediated transformation of leaf disks [S. G. Rogers, R. B. Horsch, R. T. Fraley, Methods Enzymol. 118, 627 (1988)]. Shoots that developed roots in the presence of both kanamycin and hygromycin within 14 days were considered transgenic and were maintained as shoot cuttings on plantlet medium (1/2 Murashige-Skoog, 1% sucrose). Plants were selfed, and ABP1 transgene homozygotes were selected. For the growth assay, leaf strips were removed from leaves approximately 5 cm in length and treated for 24 hours with AhTet followed by 18 hours of 1-naphthaleneacetic acid (1-NAA), then were measured. Plants were grown under greenhouse conditions.
18. R transcript and was shown to be homozygous for this transgene, was used for ABP1 transformations. T-DNA vectors pMJ10 (ABP1 gene construct in pBin-HYGO-TX) and vector-only plasmid, pBin-HYGO-TX, were used to transform R7 using standard Agrobacterium-mediated transformation of leaf disks [S. G. Rogers, R. B. Horsch, R. T. Fraley, Methods Enzymol. 118, 627 (1988)]. Shoots that developed roots in the presence of both kanamycin and hygromycin within 14 days were considered transgenic and were maintained as shoot cuttings on plantlet medium (1/2 Murashige-Skoog, 1% sucrose). Plants were selfed, and ABP1 transgene homozygotes were selected. For the growth assay, leaf strips were removed from leaves approximately 5 cm in length and treated for 24 hours with AhTet followed by 18 hours of 1-naphthaleneacetic acid (1-NAA), then were measured. Plants were grown under greenhouse conditions.
19. Antiserum directed against recombinant ABP1 was generously provided by K. Palme, Max Planck Institute, Cologne, Germany.
20. C. P. Keller and E. Van Volkenburgh, Plant Physiol. 113, 603 (1997).
21. Leaf cell volume was indirectly measured as described by A. Hemerley et al. (10). R7 and MJ10B plants having approximately four fully expanded leaves were grown under normal greenhouse conditions and watered daily with 0.1% Peter's solution (Peter's Professional, Marysville, OH) plus or minus 4 μg/ml AhTet for 13 days, a period in excess of one plastochron. The youngest, fully expanded leaf from each plant was harvested, and a 0.5-mm disk of interveinal leaf tissue was punched from the midsection of the leaf (see inset of Fig. 3) using a #2 cork borer. The cell walls of these tissues were digested for 10 hours in 25 mM MES (pH 5.6), 1/2 strength MS and Gamborg vitamins, 0.4 M sucrose, 1% cellulase (RS), and 0.5% macerase (R10). Just at this point, 75% of cells were released from the tissue and assumed a spherical shape. Yields for protoplasts were the same for all treatments. Protoplasts were photographed and the diameters determined.
22. A. Hemerley et al., EMBO J. 14, 3925 (1995); D. E. Foard and A. H. Haber, Am. J. Bot. 48, 438 (1961); D. R. Kaplan, Int. J. Plant Sci. 153, 28 (1992); _ and W. Hagemann, Bioscience 41, 693 (1991).
23. A. Hemerley et al., EMBO J. 14, 3925 (1995); D. E. Foard and A. H. Haber, Am. J. Bot. 48, 438 (1961); D. R. Kaplan, Int. J. Plant Sci. 153, 28 (1992); _ and W. Hagemann, Bioscience 41, 693 (1991).
24. A. Hemerley et al., EMBO J. 14, 3925 (1995); D. E. Foard and A. H. Haber, Am. J. Bot. 48, 438 (1961); D. R. Kaplan, Int. J. Plant Sci. 153, 28 (1992); _ and W. Hagemann, Bioscience 41, 693 (1991).
25. A. Hemerley et al., EMBO J. 14, 3925 (1995); D. E. Foard and A. H. Haber, Am. J. Bot. 48, 438 (1961); D. R. Kaplan, Int. J. Plant Sci. 153, 28 (1992); _ and W. Hagemann, Bioscience 41, 693 (1991).
26. The maize ABP1 cDNA described by U. Tillmann et al. [EMBO J. 8, 2463 (1989)] was placed in transcriptional frame with the 35S Cauliflower Mosaic viral (355 CaMV) promoter to form vector pAJ15. Linear DNA from pAJ15 containing only the 35S: ABP1 construction was cotransformed with linear DNA containing the same promoter driving the bar gene of Streptomyces hygroscopicus encoding phosphothricin acetyltransferase into maize protoplasts as described by R. Shillito et al. [Biotechnology 7, 581 (1989)]. Primary selection and screens of transformants was as described by C. Kramer, J. DiMao, G. K. Carswell, and R. D. Shillito [Planta 190, 454 (1993)]. Calli surviving on plates containing phosphothricin were then subjected to a polymerase chain reaction-based screen using ABP1-specific primers. Cells confirmed to be transformed were then subjected to immunoblot analyses using antibodies to maize ABP1. Cells were maintained on solid and in liquid 2N6 medium [C. C. Chu et al., Sci. Sin. 18, 659 (1975)].
27. The maize ABP1 cDNA described by U. Tillmann et al. [EMBO J. 8, 2463 (1989)] was placed in transcriptional frame with the 35S Cauliflower Mosaic viral (355 CaMV) promoter to form vector pAJ15. Linear DNA from pAJ15 containing only the 35S: ABP1 construction was cotransformed with linear DNA containing the same promoter driving the bar gene of Streptomyces hygroscopicus encoding phosphothricin acetyltransferase into maize protoplasts as described by R. Shillito et al. [Biotechnology 7, 581 (1989)]. Primary selection and screens of transformants was as described by C. Kramer, J. DiMao, G. K. Carswell, and R. D. Shillito [Planta 190, 454 (1993)]. Calli surviving on plates containing phosphothricin were then subjected to a polymerase chain reaction-based screen using ABP1-specific primers. Cells confirmed to be transformed were then subjected to immunoblot analyses using antibodies to maize ABP1. Cells were maintained on solid and in liquid 2N6 medium [C. C. Chu et al., Sci. Sin. 18, 659 (1975)].
28. The maize ABP1 cDNA described by U. Tillmann et al. [EMBO J. 8, 2463 (1989)] was placed in transcriptional frame with the 35S Cauliflower Mosaic viral (355 CaMV) promoter to form vector pAJ15. Linear DNA from pAJ15 containing only the 35S: ABP1 construction was cotransformed with linear DNA containing the same promoter driving the bar gene of Streptomyces hygroscopicus encoding phosphothricin acetyltransferase into maize protoplasts as described by R. Shillito et al. [Biotechnology 7, 581 (1989)]. Primary selection and screens of transformants was as described by C. Kramer, J. DiMao, G. K. Carswell, and R. D. Shillito [Planta 190, 454 (1993)]. Calli surviving on plates containing phosphothricin were then subjected to a polymerase chain reaction-based screen using ABP1-specific primers. Cells confirmed to be transformed were then subjected to immunoblot analyses using antibodies to maize ABP1. Cells were maintained on solid and in liquid 2N6 medium [C. C. Chu et al., Sci. Sin. 18, 659 (1975)].
29. The maize ABP1 cDNA described by U. Tillmann et al. [EMBO J. 8, 2463 (1989)] was placed in transcriptional frame with the 35S Cauliflower Mosaic viral (355 CaMV) promoter to form vector pAJ15. Linear DNA from pAJ15 containing only the 35S: ABP1 construction was cotransformed with linear DNA containing the same promoter driving the bar gene of Streptomyces hygroscopicus encoding phosphothricin acetyltransferase into maize protoplasts as described by R. Shillito et al. [Biotechnology 7, 581 (1989)]. Primary selection and screens of transformants was as described by C. Kramer, J. DiMao, G. K. Carswell, and R. D. Shillito [Planta 190, 454 (1993)]. Calli surviving on plates containing phosphothricin were then subjected to a polymerase chain reaction-based screen using ABP1-specific primers. Cells confirmed to be transformed were then subjected to immunoblot analyses using antibodies to maize ABP1. Cells were maintained on solid and in liquid 2N6 medium [C. C. Chu et al., Sci. Sin. 18, 659 (1975)].
30. Cells (0.2 g) were collected by filtration and extracted using a 3% SDS-containing buffer. Extraction and immunoblot analysis was performed as described (4).
31. Cells were digested overnight in 5% cellulase (RS) and macerase (R10) at 25°C with constant agitation to produce individual cells and cells in small aggregates. The nuclei were stained with 4′6′-diamidino-2-phenylindole, and the fluorescence intensity of individual nuclei was measured using a microphotometer.
32. R. M. Napier, M. A. Venis, M. A. Bolton, L. I. Richardson, G. W. Butcher, Planta 176, 519 (1988).
33. Supported, in part, by NSF (MCB-9514306) and USDA NCRICCO (9602846) to A.M.J., and by NIH (GM47369-06) to A.N.B. Special thanks to R. Napier, AFRC in UK, for providing monoclonal antibodies to maize ABP1 and S. Whitfield for assistance in preparing the illustrations.