Abnormal hippocampal spatial representations in αCaMKII(T286A) and CREB(αΔ-) mice

Science

Volumn 279, Issue 5352, 1998, Pages 867-869

  Cho, Yoon H ^a   Giese, Karl P ^b   Tanila, Heikki ^c   Silva, Alcino J ^b   Eichenbaum, Howard ^a  

^a BOSTON UNIVERSITY   (United States)

^b Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

^c UNIVERSITY OF EASTERN FINLAND   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; PROTEIN KINASE (CALCIUM,CALMODULIN); THREONINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; HIPPOCAMPUS; LEARNING DISORDER; LONG TERM POTENTIATION; MAZE TEST; MOUSE; NERVE CELL PLASTICITY; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; SPATIAL MEMORY;

ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CUES; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; HIPPOCAMPUS; LEARNING; LONG-TERM POTENTIATION; MAZE LEARNING; MICE; MICE, INBRED C57BL; MICE, MUTANT STRAINS; ORIENTATION; POINT MUTATION; PYRAMIDAL CELLS; SPACE PERCEPTION;

ANIMALIA; HIPPOCAMPUS HIPPOCAMPUS;

EID: 0032489034     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5352.867     Document Type: Article

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18. Extracellular potentials were recorded with stereotrodeor tetrode-configured bundles of 25-μm Formvar-coated nichrome wires carried by a miniature microdrive (79). Under ketamine (50 mg per kilogram of body weight) and xylazine (2 mg/kg) anesthesia, the electrode array was implanted at 1.7 mm posterior to bregma, and 1.7 mm right of the midline, just above the dorsal hippocampus. The electrode was lowered gradually each day, after which recordings were evaluated for spike unit activity. Recordings were passed through a unity gain preamplifier-headstage, amplified (10,000 times) and bandpass-filtered (0.3 to 0.6 kHz low cut, and 5 to 6 kHz high cut), and digitized at 32 kHz (Data Translation DT2821) with Enhanced Discovery software (DataWave Technologies). Animal position was tracked with a video camera system (DataWave Technologies) that followed an incandescent headstage lamp at 60 Hz. Single cells were isolated by waveform parameters (Autocut, Data-Wave Technologies), and cell isolations were considered stable when clusters remained within fixed boundaries throughout testing.
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20. 2 (four pixels of 2.37 cm by 2.37 cm) where the average firing rate was at least two times the cell's overall firing rate. By these criteria a place cell could have multiple discontiguous place fields (see Fig. 1) that were considered separately in assessments of spatial selectivity.
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24. In rats distal cues control the largest proportion of place cells (19), but in WT mice local cues predominate. However, when local cues were removed and replaced with identical smooth black arms in a separate analysis, most place cells remained spatially selective. This result indicates that although place cells in mice attend preferentially to local cues, they also respond to distal cues.
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27. Funded by grants from the Whitehall Foundation, Beckman Foundation, Klingenstein Foundation, McKnight Foundation, and NIH (AG 13622) to AJ.S., the Deutsche Forschungsgemeinschaft to K.P.G., NIH (MH51570) to H.E., and Human Frontier Science Program Fellowship to Y.H.C.