Fragmentation of a Golgi-localized chimeric protein allows detergent solubilization and reveals an alternate conformation of the cytoplasmic domain

Biochemistry

Volumn 37, Issue 1, 1998, Pages 185-192

  Sevier, Carolyn S ^a   Machamer, Carolyn E ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC PROTEIN;

ARTICLE; CONFORMATIONAL TRANSITION; GOLGI COMPLEX; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN LOCALIZATION; SOLUBILIZATION;

AMINO ACID SEQUENCE; CYTOPLASM; DETERGENTS; GOLGI APPARATUS; HELA CELLS; HEXOSAMINIDASES; HUMANS; INFECTIOUS BRONCHITIS VIRUS; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PEPTIDE FRAGMENTS; POLYMERS; PROTEIN CONFORMATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SODIUM DODECYL SULFATE; SOLUBILITY; VESICULAR STOMATITIS-INDIANA VIRUS; VIRAL ENVELOPE PROTEINS; VIRAL MATRIX PROTEINS;

EID: 0032488648     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi971782k     Document Type: Article

References (28)

1. Pfeffer, S. R., and Rothman, J. E. (1987) Annu. Rev. Biochem. 56, 829-852.
2. Mizuno, M., and Singer, S. J. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 5732-5736.
3. Balch, W. E., McCaffery, J. M., Plutner, H., and Farquhar, M. G. (1994) Cell 76, 841-852.
4. Rothman, J. E., and Wieland, F. T. (1996) Science 272, 211-234.
5. Farquhar, M. G. (1985) Annu. Rev. Cell Biol. 1, 447-486.
6. Mellman, I., and Simons, K. (1992) Cell 68, 829-840.
7. Gonatas, N. K. (1994) Am. J. Pathol. 145, 751-761.
8. Pelham, H. R. B. (1990) Trends Biochem. Sci. 15, 483-486.
9. Weisz, O. A., Swift, A. M., and Machamer, C. E. (1993) J. Cell Biol. 122, 1185-1196.
10. Nilsson, T., Hoe, M. H., Slusarewicz, P., Rabouille, C., Watson, R., Hunter, F., Watzele, G., Berger, E. G., and Warren, G. (1994) EMBO J. 13, 562-574.
11. Munro, S. (1995) EMBO J. 14, 4695-4704.
12. Nilsson, T., Rabouille, C., Hui, N., Watson, R., and Warren, G. (1996) J. Cell Sci. 109, 1975-1989.
13. Machamer, C. E., Mentone, S. A., Rose, J. K., and Farquhar, M. G. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 6944-6948.
14. Machamer, C. E., and Rose, J. K. (1987) J. Cell Biol. 105, 1205-1214.
15. Swift, A. M., and Machamer, C. E. (1991) J. Cell Biol. 115, 19-30.
16. Fuerst, T. R., Niles, E. G., Studier, F. W., and Moss, B. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 8122-8126.
17. Machamer, C. E., Grim, M. G., Esquela, A., Chung, S. W., Rolls, M., Ryan, K., and Swift, A. M. (1993) Mol. Biol. Cell 4, 695-704.
18. Rose, J. K., Adams, G. A., and Gallione, C. J. (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 2050-2054.
19. Machamer, C. E., Florkiewicz, R. Z., and Rose, J. K. (1985) Mol. Cell Biol. 5, 3074-3083.
20. Laemmli, U. K. (1970) Nature 227, 680-685.
21. Bonner, W. M., and Laskey, R. A. (1974) Eur. J. Biochem. 46, 83-88.
22. Saris, C. J. M., van Eenbergen, J., Jenks, B. G., and Bloemers, H. P. J. (1983) Anal. Biochem. 132, 54-67.
23. Lischwe, M. A., and Ochs, D. (1982) Anal. Biochem. 127, 453-457.
24. Kreis, T. E. (1986) EMBO J. 5, 931-941.
25. Schmidt, M. F. G., and Schlesinger, M. J. (1979) Cell 17, 813-819.
26. Mook, A. M. (1996) Doctoral dissertation, The Johns Hopkins University School of Medicine, Baltimore, MD.
27. Bonatti, S., Migliaccio, G., and Simons, K. (1989) J. Biol. Chem. 264, 12590-12595.
28. Mack, D., Kluxen, B., and Kruppa, J. (1989) J. Cell Biol. 109, 2057-2065.