II. Electrostatic effect in the aggregation of heat-denatured RNase A and implications for protein additive design

Biotechnology and Bioengineering

Volumn 59, Issue 3, 1998, Pages 281-285

  Tsai, Amos M ^a,b   Van Zanten, John H ^a   Betenbaugh, Michael J ^a  

^a Johns Hopkins University   (United States)

^b UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

Author keywords

Protein additives; Protein aggregation; Protein formulation; RNase A

Indexed keywords

ADDITIVES; ELECTROSTATICS; ENZYMES; MIXING; MOLECULAR ORIENTATION; MOLECULES; PH; POLYMERS; SODIUM COMPOUNDS;

CHARGED POLYMERS; DEXTRAN SULFATE; ELECTROSTATIC REPULSION; PROTEIN ADDITIVES; PROTEIN AGGREGATION; RNASE A; SODIUM DODECYL SULFATE;

BIOTECHNOLOGY;

AMPHOLYTE; DEXTRAN SULFATE; DODECYL SULFATE SODIUM; DRUG ADDITIVE; POLYANION; RIBONUCLEASE A; SULFATE;

ARTICLE; ENZYME DENATURATION; ENZYME STABILITY; MOLECULAR INTERACTION; PH; PROTEIN AGGREGATION; TEMPERATURE SENSITIVITY;

ELECTROSTATICS; HEAT; HYDROGEN-ION CONCENTRATION; KINETICS; PROTEIN DENATURATION; PROTEINS; RIBONUCLEASE, PANCREATIC;

EID: 0032486768     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980805)59:3<281::AID-BIT3>3.0.CO;2-7     Document Type: Article

References (15)

1. Anzai, Y., Mclachlan, S., Morris, M., Saxton, R., Lufkin, R. B. 1994. Dextran-coated superparamagnetic iron oxides, an MR contrast agent for assessing lymph nodes in the head and neck. Am. J. Neuroradiol. 15: 87-94.
2. Bagheri-Yarmand, R., Liu, J. F., Ledoux, D., Morere, J. F., Crepin, M. 1997. Inhibition of human breast epithelial HBL100 cell proliferation by a dextran derivative (CMDB7) with the FGF2 autocrine loop. Biochem. Biophys. Res. Commun. 239: 424-428.
3. Bockris, J. O. M., Reddy, A. K. N. 1970. Modern electrochemistry. Plenum Press, New York.
4. De Llano, J. J. M., Manning, J. M. 1994. Properties of a recombinant human hemoglobin double mutant: sickle hemoglobin with Leu-88(β) at the primary aggregation site substituted by Ala. Prot. Sci. 3: 1206-1212.
5. Hjelmeland, L. M. 1986. The design and synthesis of detergents for membrane biochemistry. Meth. Enzymol. 124: 135-164.
6. Kwong, K. W., Tsallas, G. 1980. Dilute iron dextran formulation for addition to parenteral nutrient solutions. Am. J Hosp. Pharm. 37: 206-210.
7. Manning, M. C., et al. 1995. Approaches for increasing the solution stability of proteins. Biotechnol. Bioeng. 48: 506-512.
8. Olbricht, C. J. 1996. Extracorporeal removal of lipids by dextran sulfate cellulose adsorption. Artif. Organs 20: 332-335.
9. Qi, H., Heller, D. L. 1995. Stability and stabilization of insulinotropin in a dextran formulation. PDA J. Pharm. Sci. Technol. 49: 289-293.
10. Reynolds, J. A., Tanford, J. 1970. The gross conformation of protein-sodium dodecyl sulfate complexes. J. Biol. Chem. 245: 5161-5165.
11. Takagi, T., Tsuji, K., Shirahama, K. 1975. Binding isotherms of sodium dodecyl sulfate to protein polypeptides with special reference to SDS-polyacrylamide gel electrophoresis. J. Biochem. 77: 939-947.
12. Tanford, C., Hauenstein, J. D. 1956. Hydrogen ion equilibria of ribonuclease. J. Am. Chem. Soc. 78: 5287-5291.
13. Timasheff, S. N. 1992. pp. 265-285. In: T. J. Ahern and M. C. Manning (eds.), Stability of protein pharmaceuticals. Plenum Press, New York.
14. Tsai, A.M., van Zanten, J. H., Betenbaugh, M.J. 1998. The study of protein aggregation due to heat denaturation: a structural approach using circular dichroism spectroscopy, nuclear magnetic resonance, and static light scattering. Biotechnol. Bioeng. 59: 000-000.
15. Yoshioka, S., Aso, Y., Kojuma, S. 1997. Dependence of the molecular mobility and protein stability of freeze-dried gamma-globulin formulations on the molecular weight of dextran. Pharm. Res. 14: 736-741.