Structure-based redesign of the catalytic/metal binding site of Cfr10I restriction endonuclease reveals importance of spatial rather than sequence conservation of active centre residues

Journal of Molecular Biology

Volumn 279, Issue 2, 1998, Pages 473-481

  Skirgaila, Remigijus ^a   Grazulis, Saulius ^a,b   Bozic, Damir ^b   Huber, Robert ^b   Siksnys, Virginijus ^a  

^a VILNIUS UNIVERSITY   (Lithuania)

^b MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

Active site carboxylates; Cfr10I restriction endonuclease; Site directed mutagenesis

Indexed keywords

GLUTAMIC ACID; RESTRICTION ENDONUCLEASE; SERINE;

ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME STRUCTURE; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

EID: 0032486108     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1998.1803     Document Type: Article

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