Synthesis of immobilized CMP-sialic acids and their enzymatic transfer with sialyltransferase

Angewandte Chemie - International Edition

Volumn 37, Issue 22, 1998, Pages 3166-3169

  Kajihara, Yasuhiro ^a,b   Ebata, Takashi ^b   Kodama, Hisashi ^b  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

^b JAPAN TOBACCO INC   (Japan)

Author keywords

Enzymes; Glycoproteins; Immobilization; Sialic acids; Solid phase synthesis

Indexed keywords

ASIALOGLYCOPROTEIN; CYTIDINE MONOPHOSPHATE SIALIC ACID; ENZYMATIC TRANSFER; OLIGOSACCHARIDE; PROTEIN IMMOBILIZATION; SIALYLTRANSFERASE;

GALACTOSIDE; LECTIN; OLIGOSACCHARIDE; SIALIC ACID; SIALYLTRANSFERASE;

ARTICLE; CHEMICAL BOND; CHEMICAL STRUCTURE; ELECTRON TRANSPORT; PROTON NUCLEAR MAGNETIC RESONANCE; REACTION ANALYSIS; SIALYLATION; STRUCTURE ANALYSIS; SYNTHESIS;

EID: 0032484068     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1521-3773(19981204)37:22<3166::AID-ANIE3166>3.0.CO;2-I     Document Type: Article

References (20)

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13. Prior to the assays, 7 was washed with BSA (2 mg of BSA in 1 mL of HEPES buffer) in a 1.5-mL eppendorf tube in order to prevent nonspecific absorption of STase or glycoprotein acceptor to the CPG. After centrifugation, the supernatant was removed. This treatment was repeated three times, and then 7 was washed with water. After lyophylization, CMP-Neu5Ac 7 was used for assays.
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15. 14C]-LacNAc incorporated on the CPG was estimated. The transfer rates after subtraction of control values are summarized in Figure 3 (panels A-F). The data given (panels A-H) are averages values.
16. [15]
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19. Immobilization of the STase as a side reaction of this method could not be detected in several self-immobilization assays of STase.
20. Immobilization yields are estimated to be about 4.5 % and 5.8 % in the case of LacNAc and asialoglycoprotein, respectively, based on the sialylacceptors added.