Use of the comet assay to investigate the role of superoxide in glutathione-induced DNA damage

Biochemical and Biophysical Research Communications

Volumn 243, Issue 1, 1998, Pages 241-245

  Thomas, Steven ^a   Lowe, Jillian E ^a   Hadjivassiliou, Vicky ^a   Knowles, Richard G ^b   Green, Irene C ^a   Green, Michael H L ^a  

^a UNIVERSITY OF SUSSEX   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Comet assay (single cell gel electrophoresis); DNA strand break; Glutathione; Hydrogen peroxide; Nitric oxide; S nitrosoglutathione; Superoxide

Indexed keywords

CATALASE; DNA; EDETIC ACID; GLUTATHIONE; PENTETIC ACID; S NITROSOGLUTATHIONE; SUPEROXIDE; SUPEROXIDE DISMUTASE;

ARTICLE; CONTROLLED STUDY; DNA DAMAGE; DNA DETERMINATION; DNA STRAND BREAKAGE; HUMAN; HUMAN CELL; PHOTOLYSIS; PRIORITY JOURNAL; ULTRAVIOLET A RADIATION;

EID: 0032481282     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.1997.8000     Document Type: Article

References (20)

1. Singh N. P., McCoy M. T., Tice R. R., Schneider E. L. Expl. Cell Res. 175:1988;184-191.
2. Ostling O., Johanson K. J. Biochem. Biophys. Res. Commun. 123:1984;291-298.
3. Green M. H. L., Lowe J. E., Delaney C. A., Green I. C. Meth. Enzymol. 269:1996;243-266.
4. Kasamatsu T., Kohda K., Kawazoe Y. Mutation Res. 396:1996;1-6.
5. Halliwell B., Gutteridge J. M. C. Free Radicals in Biology and Medicine. 1989;Clarendon Press, Oxford.
6. Meister A. Meth. Enzymol. 251:1995;3-8.
7. Meister A., Anderson M. E. Ann. Rev. Biochem. 52:1983;711-760.
8. Winterbourn C. C., Metodiewa D. Meth. Enzymol. 251:1995;81-86.
9. Sah N. K., Kumar S., Subramanian M., Devasagayam T. P. A. Biochem. Molec. Biol. Intl. 35:1995;291-296.
10. Keyer K., Imlay J. A. Proc. Natl. Acad. Sci. USA. 93:1996;13635-13640.
11. Brawn K., Fridovich I. Arch. Biochem. Biophys. 206:1981;414-419.
12. Cole J., Green M. H. L., James S. E., Henderson L., Cole H. Mutation Res. 204:1988;493-507.
13. Green M. H. L., Waugh A. P. W., Lowe J. E., Harcourt S. A., Cole J., Arlett C. F. Mutation Res. 315:1994;25-32.
14. Collins A. R., Duthie S. J., Dobson V. L. Carcinogenesis. 14:1993;1733-1735.
15. Winterbourn C. C., Metodiewa D. Arch. Biochem. Biophys. 314:1994;284-290.
16. Murphy M. E., Sies H. Proc. Natl. Acad. Sci. USA. 88:1991;10860-10864.
17. Beckman J., Tsai J.-H. Biochemist. 16:1994;8-10.
18. Beckman J. S., Beckman T. W., Chen J., Marshall P. A., Freeman B. A. Proc. Natl. Acad. Sci. USA. 87:1990;1620-1624.
19. Rosen D. R., Siddique T., Patterson D., Figlewicz D. A., Sapp P., Hentati A., Donaldson D., Goto J., O'Regan J. P., Deng H.-X., Rahmani Z., Krizus A., McKenna-Yasek D., Cayabyab A., Gaston S. M., Berger R., Tanzi R. E., Halperin J. J., Herzfeldt B., Van den Bergh R., Hung W.-Y., Bird T., Deng G., Mulder D. W., Smyth C., Laing N. G., Soriano E., Pericack-Vance M. A., Haines J., Rouleau G. A., Gusella J. S., Horvitz H. R., Brown R. H. Nature. 362:1993;59-62.
20. Beckman J. S., Carson M., Smith C. D., Koppenol W. H. Nature. 364:1993;584.