Fusion pore expansion in horse eosinophils is modulated by Ca2+ and protein kinase C via distinct mechanisms

EMBO Journal

Volumn 17, Issue 15, 1998, Pages 4340-4345

  Scepek, Susanne ^a   Coorssen, Jens R ^b   Lindau, Manfred ^c  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^c Cornell University   (United States)

Author keywords

Capacitance; Exocytosis; Membrane fusion; Patch clamp; Staurosporine

Indexed keywords

CALCIUM ION; PHORBOL 13 ACETATE 12 MYRISTATE; PROTEIN KINASE C;

ANIMAL CELL; ARTICLE; DEPHOSPHORYLATION; EOSINOPHIL; EXOCYTOSIS; HORSE; MEMBRANE FUSION; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SECRETORY GRANULE;

ANIMALS; CALCIUM; CELL DEGRANULATION; CELL MEMBRANE; EOSINOPHILS; EXOCYTOSIS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); HORSES; MEMBRANE FUSION; NAPHTHALENES; ORGANOPHOSPHORUS COMPOUNDS; PATCH-CLAMP TECHNIQUES; PROTEIN KINASE C; TETRADECANOYLPHORBOL ACETATE; TIME FACTORS;

ANIMALIA; EQUUS CABALLUS;

EID: 0032480012     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.15.4340     Document Type: Article

References (44)

1. Ämmälä, C., Eliasson, L., Bokvist, K., Berggren, P.O., Honkanen, R.E., Sjoholm, A. and Rorsman, P. (1994) Activation of protein kinases and inhibition of protein phosphatases play a central role in the regulation of exocytosis in mouse pancreatic beta cells. Proc. Natl Acad. Sci. USA, 91, 4343-4347.
2. Breckenridge, L.J. and Almers, W. (1987) Currents through the fusion pore that forms during exocytosis of a secretory vesicle. Nature, 328, 814-817.
3. Brewer, C.B. and Roth, M.G. (1995) Polarized exocytosis in MDCK cells is regulated by phosphorylation. J. Cell Sci. 108, 789-769.
4. Brooks, S.P.J. and Storey, K.B. (1992) Bound and determined - a computer program for making buffers of defined ion concentrations. Anal. Biochem., 201, 119-126.
5. Brose, N., Hofmann, K., Hata, Y. and Sudhof, T.C. (1995) Mammalian homologs of Caenorhabditis elegans unc-13 gene define novel family of C-2 domain proteins. J. Biol. Chem., 270, 25273-25280.
6. Budworth, J. and Gescher, A. (1995) Differential inhibition of cytosolic and membrane-derived protein kinase C activity by staurosporine and other kinase inhibitors. FEBS Lett., 362, 139-142.
7. Churcher, Y. and Gomperts, B.D. (1990) ATP-dependent and ATP-independent pathways of exocytosis revealed by interchanging glutamate and chloride as the major anion in permeabilized mast cells. Cell Regul., 1, 337-345.
8. Coorssen, J.R. (1996) Phospholipase activation and secretion: evidence that PLA-2, PLC and PLD are not essential to exocytosis. Am. J. Physiol., 270, C1153-C1163.
9. 2+-independent secretion and phospholipase D activity in permeabilized human platelets. FEBS Lett., 316, 170-174.
10. Coorssen, J.R., Davidson, M.M.L. and Haslam, R.J. (1990) Factors affecting dense and alpha-granule secretion from electropermeabilized human platelets: calcium independent actions of phorbol ester and GTP-γ-S. Cell Regul., 1, 1027-1042.
11. Coorssen, J.R., Davison, M.M.L. and Haslam, R.J. (1992) Correlations between protein phosphorylation, phospholipase D activity and calcium-independent secretion from permeabilized platelets. Biophys. J., 61, A225.
12. 2+ triggers massive exocytosis in Chinese hamster ovary cells. EMBO J., 15, 3787-3791.
13. Feigenson, G.W. (1986) On the nature of calcium ion binding between phosphatidylserine lamellae. Biochemistry, 25, 5819-5825.
14. Fernandez, J.M., Neher, E. and Gomperts, B.D. (1984) Capacitance measurements reveal stepwise fusion events in degranulating mast cells. Nature. 312, 453-455.
15. Fernández-Chacón, R. and Alvarez de Toledo, G. (1995) Cytosolic calcium facilitates release of secretory products after exocytotic vesicle fusion. FEBS Lett., 363, 221-225.
16. Fidler, N. and Fernandez, J.M. (1989) Phase tracking: an improved phase detection technique for cell membrane capacitance measurements. Biophys. J., 56, 1153-1162.
17. Gillis, K.D., Mößner, R. and Neher, E. (1996) Protein kinase C enhances exocytosis from chromaffin cells by increasing the size of the readily releasable pool of secretory granules. Neuron. 16, 1209-1220.
18. E: a GTP-binding protein mediating exocytosis. Annu. Rev. Physiol., 52, 591-606.
19. 2+-dependent step in exocytosis subsequent to vesicle fusion. FEBS Lett., 363, 217-220.
20. Hay, J.C., Fisette, P.L., Jenkins, G.H., Fukami, K., Takenawa, T., Anderson, R.A. and Martin, T.F.J. (1995) ATP-dependent inositide phosphorylation required for Ca(2+)-activated secretion. Nature, 374, 173-177.
21. Henkel, A.W. and Betz, W.J. (1995) Staurosporine blocks evoked release of FM1-43 but not acetylcholine from frog motor nerve terminals. J. Neurosci., 15, 8246-8258.
22. 2+ and guanine nucleotides: an all-or-none event. J. Cell Biol. 123, 585-593.
23. 2+ and GTPγS for exocytosis from permeabilised mast cells. Cell. Signal., 1, 157-163.
24. Koopman, W.R. and Jackson, R.C. (1990) Calcium- and guanine-nucleotide-dependent exocytosis in permeabilized rat mast cells. Biochem. J., 265, 365-373.
25. Lindau, M. (1991) Time-resolved capacitance measurements - monitoring exocytosis in single cells. Quart. Rev. Biophys., 24, 75-101.
26. Lindau, M. and Almers, W. (1995) Structure and function of fusion pores in exocytosis and ectoplasmic membrane fusion. Curr. Opin. Cell Biol., 7, 509-517.
27. Lindau, M. and Gomperts, B.D. (1991) Techniques and concepts in exocytosis: focus on mast cells. Biochim. Biophys. Acta. 1071, 429-171.
28. Lindau, M. and Neher, E. (1988) Patch-clamp techniques for time-resolved capacitance measurements in single cells. Pflügers Arch. Eur. J. Physiol., 411, 137-146.
29. Lindau, M., Hartmann, J. and Scepek, S. (1994) Three distinct fusion processes during eosinophil degranulation. Ann. NY Acad. Sci., 710, 232-247.
30. Monck, J.R., Alvarez de Toledo, G. and Fernandez, J.M. (1990) Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore. Proc. Natl Acad. Sci. USA, 87, 7804-7808.
31. Murthy, C.N. and Stephens, C.F. (1998) Synaptic vesicles retain their identity through the endocytic cycle. Nature, 392, 497-501.
32. Nanavati, C., Markin, V.S., Oberhauser, A.F. and Fernandez, J.M. (1992) The exocytotic fusion pore modeled as a lipidic pore. Biophys. J., 63, 1118-1132.
33. Neher, E. and Marty, A. (1982) Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells. Proc. Natl Acad. Sci. USA, 79, 6712-6716.
34. Niessen, H.W.M. and Verhoeven, A.J. (1994) Role of protein phosphorylation in the degranulation of electropermeabilized human neutrophils. Biochim. Biophvs. Acta, 1223, 267-273.
35. Nüße, O. and Lindau, M. (1988) The dynamics of exocytosis in human neutrophils. J. Cell Biol., 107, 2117-2124.
36. Nüße, O., Lindau, M., Cromwell, O., Kay, A.B. and Gomperts, B.D. (1990) Intracellular application of guanosine-5′O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils. J. Exp. Med., 171, 775-786.
37. Santini, F. and Beaven, M.A. (1993) Tyrosine phosphorylation of a mitogen-activated protein kinase-like protein occurs at a late step in exocytosis. Studies with tyrosine phosphatase inhibitors and various secretagogues in rat RBL-2H3 cells. J. Biol. Chem., 268, 22716-22722.
38. Scepek, S. and Lindau, M. (1993) Focal exocytosis by eosinophils: compound exocytosis and cumulative fusion. EMBO J., 12, 1811-1817.
39. Smith, C. and Neher, E. (1997) Multiple forms of endocytosis in bovine adrenal chromaffin cells. J. Cell Biol., 139, 885-894.
40. Spruce, A.E., Breckenridge, L.J., Lee, A.K. and Almers, W. (1990) Properties of the fusion pore that forms during exocytosis of a mast cell secretory vesicle. Neuron. 4, 643-654.
41. Terbush, D.R. and Holz, R.W. (1990) Activation of protein kinase C is not required for exocytosis from bovine adrenal chromaffin cells. J. Biol. Chem., 265, 21179-21184.
42. Wiedemann, C., Schäfer, T. and Burger, M.M. (1996) Chromaffin granule-associated phosphatidylinositol 4-kinase activity is required for stimulated secretion. EMBO J., 15, 2094-2101.
43. Yamane, H.K. and Fung, B.K.K. (1993) Covalent modifications of G proteins. Annu. Rev. Pharmacol. Toxicol., 33, 201-241.
44. Zieseniss, E. and Plattner, H. (1985) Synchronous exocytosis in Paramecium cells involves very rapid (less than or equal to 1 s), reversible dephosphorylation of a 65-kD phosphoprotein in exocytosis-competent strains. J. Cell Biol., 101, 2028-2035.