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-4 M. Seven seconds into the dissociation phase, monitored for 2 min in free buffer flow, report points were taken to generate dose-response curves for estimating IC50 values; ie., the inhibitor concentrations causing a signal decrease of 50 percent. The sensorchip surfaces were very stable and could after sample injection be regenerated using 0.05 % (w/v) SDS.
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0010581366
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note
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3CN/TFA 50/50/0.05 within 15 min. at 60°C, 1 ml/min.) Under these conditions, 8 had a retention time of 9.2 min.
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