Leaves of transgenic tomato plants overexpressing prosystemin accumulate high levels of cystatin

Plant Science

Volumn 138, Issue 1, 1998, Pages 35-42

  Jacinto, Tânia ^a   Fernandes, Kátia V S ^b   MacHado, Olga L T ^b   Siqueira Júnior, César L ^a  

^a Laboratorio de Biotecnologia   (Brazil)

^b Lab Quim Funcao Proteinas P   (Brazil)

Author keywords

Cystatin; Inhibitor constant (K(i)); Lycopersicon esculentum; Papain; Systemin

Indexed keywords

CYSTATIN; CYSTEINE PROTEINASE INHIBITOR;

AFFINITY CHROMATOGRAPHY; ARTICLE; CROSS REACTION; DISULFIDE BOND; DRUG ISOLATION; ENZYME INDUCTION; ENZYME INHIBITION; NONHUMAN; PLANT LEAF; TOMATO; TRANSGENIC PLANT;

LYCOPERSICON ESCULENTUM;

EID: 0032476613     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(98)00153-8     Document Type: Article

References (41)

1. Ryan C.A. Proteinase inhibitors. Marcus A. The Biochemistry of Plants. A Comprehensive Treatise: Proteins and Nucleic Acids. 6:1981;351-370 Academic Press, New York.
2. Ryan C.A. Proteinase inhibitors in plant: genes for improving defenses against insects and pathogens. Annu. Rev. Phytopath. 28:1990;425-449.
3. Pearce G., Strydom D., Johnson S., Ryan C.A. A polypeptide from tomato leaves actives the expression of proteinase inhibitor genes. Science. 253:1991;895-897.
4. Bergey D.R, Howe G.A., Ryan C.A. Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals. PNAS USA. 93:1996;12053-12058.
5. McGurl B., Pearce G., Orozco-Cardenas M., Ryan C.A. Structure, expression, and antisense inhibition of the systemin precursor gene. Science. 255:1992;1570-1573.
6. McGurl B., Orozco-Cardenas M., Pearce G., Ryan C.A. Overexpression of the prosystemin gene in transgenic tomato plants generates a systemic signal that constitutively induces proteinase inhibitor synthesis. PNAS USA. 91:1994;9799-9802.
7. Abe K., Emori Y., Kondo H., Suzuki K., Arai S. Molecular cloning of a cysteine proteinase inhibitor of rice (oryzacystatin). J. Biol. Chem. 262:1987;16793-16797.
8. Abe K., Kondo H., Arai S. Purification and characterization of a rice cysteine protease inhibitor. Agric. Biol. Chem. 51:1987;2763-2768.
9. Abe M., Abe K., Kuroda M., Arai S. Corn kernel cysteine proteinase inhibitor as a novel cystatin superfamily member of plant origin. Eur. J. Biochem. 209:1992;933-937.
10. Hines M.E., Osuala C.I., Nielsen S.S. Isolation and partial characterization of a soybean cystatin cysteine proteinase inhibitor of coleopteran digestive proteolytic activity. J. Agric. Food Chem. 39:1991;1515-1520.
11. Fernandes K.V.S., Campos F.A.P., Do Val R.R., Xavier-Filho J. The expression of papain inhibitors during development of cowpea seeds. Plant Sci. 74:1991;179-184.
12. Fernandes K.V.S., Sabelli P.A., Barratt D.H.P., Richardson M., Xavier-Filho J., Shewry P.R. The resistance of cowpea seeds to bruchid beetles is not related to levels of cysteine proteinase inhibitors. Plant Mol. Biol. 23:1993;215-219.
13. Lim C.O., Lee S.I., Chung W.S., Park S.H., Hwang I., Cho M.J. Characterization of a cDNA encoding cysteine proteinase inhibitor from chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Plant Mol. Biol. 30:1996;373-379.
14. Waldron C., Wegrich L.M., Merlo P.A.O., Walsh T.A. Characterization of a genomic sequence coding for potato multicystatin, an eight-domain cysteine proteinase inhibitor. Plant Mol. Biol. 23:1993;801-812.
15. Murdock L.L., Brookhart G., Dunn P.E., Foard D.E., Kelley S., Kitch L., Shade R.E., Shukle R.H., Wolfson J.L. Cysteine digestive proteinase in Coleoptera. Comp. Biochem. Physiol. 87B:1987;783-787.
16. Liang C., Brookhart G., Feng G.H., Kramer K.J. Inhibition of digestive proteinases of stored grain coleoptera by oryzacystatin, a cystatin proteinase inhibitor from rice seeds. FEBS Lett. 278:1991;139-142.
17. Chen M.S., Johnson B., Wen L., Muthukrishnan S., Kramer K-J., Morgan T.D., Reeck G.R. Rice cystatin: bacterial expression, purification, cysteine proteinase inhibitory activity, and insect growth suppressing activity of a truncated form of the protein. Prot. Purif. Express. 3:1992;41-49.
18. Michaud D., Nguyen-Quoc B., Yelle S. Selective inhibition of Colorado potato beetle cathepsin H by oryzacystatins I and II. FEBS Lett. 331:1993;173-176.
19. Orr G.L., Strickland J.A., Walsh T.A. Inhibition of Diabrotica larval growth by a multicystatin from potato tubers. J. Insect Physiol. 40:1994;893-900.
20. Kuroda M., Ishimoto M., Suzuki K., Kondo H., Abe K., Kimatura K., Arai S. Oryzacystatin exhibit growth-inhibitory and lethal effects on different species of bean insect pests, Callosobruchus chinensis (Coleoptera) and Riptortus clavatus (Hemiptera). Biosci. Biotech. Biochem. 60:1996;209-212.
21. Cox G.N., Pratt D., Hageman R., Boisvenue R.J. Molecular cloning and primary sequence of a cysteine protease expressed by Haemonchus contortus adult worms. Mol. Biochem. Parasitol. 41:1990;25-34.
22. Bolter C.J. Methyl jasmonate induces papain inhibitors(s) in tomato leaves. Plant Physiol. 103:1993;1347-1353.
23. Zhao Y., Botella M.A., Subramanian L., Niu X., Nielsen S.S., Bressan R.A., Hasegawa P.M. Two wound-inducible soybean cysteine proteinase inhibitors have greater insect digestive proteinase inhibitory activities than a constitutive homolog. Plant Physiol. 111:1996;1299-1306.
24. Benchekroun A., Michaud D., Nguyen-Quoc B., Overney S., Desjardins Y., Yelle S. Synthesis of active oryzacystatin I in transgenic potato plants. Plant Cell Rep. 14:1995;585-588.
25. Leplé J-C., Bonadé-Bottino M., Augustin S., Delplanque A., Dumanois V., Pilate G., Cornu D., Jouanin L. Toxicity to Chrysomela tremulae (Coleoptera: Chrysomelidae) of transgenic poplars expressing a cysteine proteinase inhibitor. Mol. Breed. 1:1995;319-328.
26. Irie K., Hosoyama H., Takeuchi T., Iwabuchi K., Watanabe H., Abe M., Abe K., Arai S. Transgenic rice established to express corn cystatin exhibits strong inhibitory activity against insect gut proteinases. Plant Mol. Biol. 30:1996;149-157.
27. Urwin P.E., Lilley C.J., McPherson M.J., Atkinson H.J. Resistance to both cyst and root-knot nematodes conferred by transgenic Arabidopsis expressing a modified plant cystatin. Plant J. 12:1997;455-461.
28. T. Jacinto, V.M. Gomes, O.L.T. Machado, P.D. Ribeiro, R. Louro, C.A. Ryan, K.V.S. Fernandes, Immunodetection of a cysteine proteinase inhibitor homologue in transgenic tomato plants that overexpress the prosystemin gene, XXV Annu. Meet. of the Brazilian Society of Biochemistry and Molecular Biology, E51 abstract, 1996, p. 38.
29. Michaud D., Nguyen-Quoc B., Bernier-Vadnais N., Faye L., Yelle S. Cysteine proteinase forms in sprouting potato tuber. Physiol. Plant. 90:1994;497-503.
30. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
31. S. Bozzo, C. Retamal, Gel-Perfect: Un nuevo método densitográfico para geles unidimensionales, 34th Annu. Meet. of the Society of Biology of Chile and the Associated Societies, Arch. Biol. Med. Exp. 24 (1991) R-181 abstract.
32. Towbin K., Staehelin T., Gordon J. Electrophorectic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. PNAS USA. 76:1979;4350-4354.
33. K.V.S. Fernandes, Characterisation and molecular cloning of a cysteine proteinase inhibitor from seeds of cowpea (Vigna unguiculata), Ph.D. Thesis, University of Bristol, UK, 1992.
34. Constabel P., Bergey D., Ryan C.A. Systemin activates synthesis of wound-inducible tomato leaf polyphenol oxidase via the octadecanoid defense signaling pathway. PNAS USA. 92:1995;407-411.
35. Anastasi A., Brown M.A., Kembhavi A.A., Nicklin M.J.H, Sayers C.A., Sunter D.C., Barret A.J. Cystatin, a protein inhibitor of cysteine proteinases. J. Biochem. 211:1983;129-138.
36. Ausebel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Strulh K. Current Protocols in Molecular Biology. 1994;Wiley, New York.
37. Abe M., Abe K., Iwabuchi K., Domoto C., Arai S. Corn cystatin-I expressed in Escherichia coli: investigation of its inhibitory profile and occurence in corn kernels. J. Biochem. 116:1994;488-492.
38. Arai S., Watanabe H., Kondo H., Emori Y., Abe K. Papain-inhibitory activity of oryzacystatin, a rice seed cysteine protease inhibitor, depends on the central Gln-Val-Val-Ala-Gly region conserved among cystatin superfamily members. J. Biochem. 109:1991;294-298.
39. Urwin P.E., Atkinson H.J., Waller D.A., McPherson J. Engineered oryzacystatin-I expressed in hairy roots confers resistance to Globodera pallida. Plant J. 8:1995;121-131.
40. 2-terminal 21 amino acid residues are not essential for the papain inhibitory activity of oryzacystatin, a member of the cystatin superfamily. J. Biol. Chem. 263:1988;7655-7659.
41. Kondo H., Abe K., Nishimura I., Watanabe H., Emori Y., Arai S. Two distinct cystatin species in rice seeds with different specificities against cysteine proteinases. J. Biol. Chem. 265:1990;15832-15837.