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note
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4 (4%)-seawater mixture (1:1). The animals were stained with uranyl acetate en bloc prior to embedding in Epon 812. Serial sections for light and transmission electron microscopy (TEM) were prepared on an LKB ultratome 1 as follows: a survey series of about 20 semi-thin sections 0.5 μm thick was made, followed by a series of ultra-thin sections 0.05 μm in thickness. This pattern was repeated separately for the length and height of the entire animals. Sections were stained with lead citrate (1 min) for TEM and with toluidine blue for light microscopy. TEM micrographs were prepared with a Siemens Elmiskop 102.
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