A highly selective, high-affinity transporter for uracil in Trypanosoma brucei brucei: Evidence for proton-dependent transport

Biochemistry and Cell Biology

Volumn 76, Issue 5, 1998, Pages 853-858

  De Koning, Harry P ^a   Jarvis, Simon M ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

Author keywords

Nucleobase transport; Proton nucleobase cotransport; Trypanosome; Uracil

Indexed keywords

POTASSIUM ION; PROTON; PURINE DERIVATIVE; PYRIMIDINE DERIVATIVE; SODIUM CHLORIDE; SODIUM ION; URACIL;

CONCENTRATION RESPONSE; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME INHIBITION; ENZYME ISOLATION; ENZYME SPECIFICITY; NONHUMAN; NUCLEOSIDE TRANSPORT; POTASSIUM CELL LEVEL; SODIUM CELL LEVEL; TRANSPORT KINETICS; TRYPANOSOMA BRUCEI;

ANIMALS; CARRIER PROTEINS; DOSE-RESPONSE RELATIONSHIP, DRUG; KINETICS; NUCLEOSIDES; TIME FACTORS; TRYPANOSOMA BRUCEI BRUCEI; URACIL;

PROTOZOA; TRYPANOSOMA; TRYPANOSOMA BRUCEI BRUCEI;

EID: 0032461771     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/bcb-76-5-853     Document Type: Conference Paper

References (19)

1. Brun, R., and Schönenberger, M. 1979. Cultivation and in vivo cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Trop. 36: 289-292.
2. Burton, K. 1977. Transport of adenine, hypoxanthine and uracil into Escherichia coli. Biochem. J. 168: 195-204.
3. Burton, K. 1983. Transport of nucleic acid bases into Escherichia coli. J. Gen. Microbiol. 129: 3505-3513.
4. Carter, N.S., and Fairlamb, A.H. 1993. Arsenical-resistant trypanosomes lack an unusual adenosine transporter. Nature (London), 361: 173-175.
5. De Koning, H.P., and Jarvis, S.M. 1997a. Hypoxanthine uptake through a purine-selective nucleobase transporter in Trypanosoma brucei brucei procyclic cells is driven by protonmotive force. Eur. J. Biochem. 247: 1102-1110.
6. De Koning, H.P., and Jarvis, S.M. 1997b. Purine nucleobase transport in bloodstream forms of Trypanosoma brucei brucei is mediated by two novel transporters. Mol. Biochem. Parasitol. 89: 245-258.
7. De Koning, H.P., Watson, C.J., and Jarvis, S.M. 1998. Characterization of a nucleoside/proton symporter in procyclic Trypanosoma brucei brucei. J. Biol. Chem. 273: 9486-9494.
8. Domin, B.A., Mahoney, W.B., and Zimmerman, T.P. 1993. Transport of 5-fluorouracil and uracil into human erythrocytes. Biochem. Pharmacol. 46: 503-510.
9. Eddy, A.A. 1982. Mechanisms of solute transport in selected eukaryotic microorganisms. Adv. Microb. Physiol. 23: 1-78.
10. Griffith, D.A., and Jarvis, S.M 1994. Characterization of a sodium-dependent concentrative nucleobase-transport system in guinea-pig kidney cortex brush-border membrane vesicles. Biochem. J. 303: 901-905.
11. Griffith, D.A., and Jarvis, S.M 1996. Nucleoside and nucleobase transport systems of mammalian cells. Biochim. Biophys. Acta, 1286: 153-181.
12. Gutteridge, W.E., and Coombs, G.H. 1977. Biochemistry of parasitic protozoa. MacMillan Press, London.
13. Hammond, D.J., and Gutteridge, W.E. 1984. Purine and pyrimidine metabolism in the trypanosomatidae. Mol. Biochem. Parasitol. 13: 243-261.
14. Hassan, H.F., and Coombs, G.H. 1988. Purine and pyrimidine metabolism in parasitic protozoa. FEMS Microbiol. Rev. 54: 47-84.
15. Hopkins, P., Chevallier, N.R., Jund, R., and Eddy, A.A. 1988. Use of plasmid vectors to show that the uracil and cytosine permeases of the yeast Saccharomyces cerevisiae are electrogenic proton symports. FEMS Microbiol. Lett. 49: 173-177.
16. Rubin, R.J., Jaffe, J.J., and Handschumacher, R.E. 1962. Quantitative differences in the pyrimidine metabolism of Trypanosoma equiperdum and mammals as characterized by 6-azauracil and 6-azauridine. Biochem. Pharmacol. 11: 563-572.
17. Wang, C.C. 1983. Purine and pyrimidine metabolism in Trichomonadae and Giardia: potential targets for chemotherapy. In Biochemical basis of drug action. Edited by T.P. Singer, T. Mansour, and R. Ondarza. Academic Press, New York. pp. 133-145.
18. +-dependent nucleobase transporter with broad substrate selectivity. J. Biol. Chem. 270: 22 816-22 819.
19. Yuasa, H., Matsuhisa, E., and Watanabe, J. 1996. Intestinal brush border transport mechanism of 5-fluorouracil in rats. Biol. Pharm. Bull. 19: 94-99.