Percoll density gradient-enriched populations of rat pituitary cells: Interleukin 6 secretion, proliferative activity, and nitric oxide synthase expression

Endocrine Pathology

Volumn 9, Issue 4, 1998, Pages 339-346

  Qian, Xiang ^a   Jin, Long ^a   Lloyd, Ricardo V ^a  

^a MAYO CLINIC   (United States)

Author keywords

Cell separation; Interleukin 6; Nitric oxide synthase; Pituitary

Indexed keywords

INTERLEUKIN 6; NITRIC OXIDE SYNTHASE;

ANIMAL CELL; ARTICLE; CELL POPULATION; CELL PROLIFERATION; DENSITY GRADIENT CENTRIFUGATION; HYPOPHYSIS CELL; NONHUMAN; PRIORITY JOURNAL; RAT;

EID: 0032458647     PISSN: 10463976     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02739694     Document Type: Article

References (30)

1. Hymer WC, Hatfield JM. Separation of cells from the rat anterior pituitary gland. Methods Enzymol 103:257-287, 1983.
2. Lloyd RV, McShan WH. Study of rat anterior pituitary cells separated by velocity sedimentation at unit gravity. Endocrinology 92:1639-1651, 1973.
3. Denef C, Hantekeete E, DeWolf A, Vanderschueren B. Pituitary basophils from immature male and female rats; distribution of gonadotroph and thyrotrophs as studied by unit gravity sedimentation. Endocrinology 103:724-735, 1978.
4. Denef C, Andries M. Evidence for paracrine interactions between gonadotrophs and lactotrophs in pituitary cell aggregates. Endocrinology 112:813-822, 1983.
5. Vankelcom H, Matthys P, Denef C. Inducible nitric oxide synthase in the anterior pituitary gland: induction by interferongamma in a subpopulation of folliculostellate cells and in an unidentifiable population of non-hormone-secreting cells. J Histochem Cytochem 45:847-857, 1997.
6. Velkeniers B, Hooghe-Peters EL, Hooghe R, Belayew A, Smets G, Claeys A, et al. Prolactin cell subpopulations separated on discontinuous Percoll gradient: an immunocytochemical, biochemical, and physiological characterization. Endocrinology 123:1619-1630, 1988.
7. Hall M, Howell SL, Schulster D, Wallis M. A procedure for the purification of somatotrophs isolated from rat anterior pituitary glands using Percoll density gradients. J Endocrinol 94:257-266, 1982.
8. Gomm JJ, Ray KP, Wallis M. Purification of ovine somatotrophs using a combination of density gradient centrifugation and short-term culture. J Endocrinol 115:395-403, 1987.
9. Marin F, Stefaneanu L, Kovacs K. Folliculo-stellate cells of the pituitary. Endocr Pathol 2:180-192, 1991.
10. Horvath E, Kovacs K, Perez G, Ezrin C. Origin, possible function and fate of "follicular cells" in the anterior lobe of the human pituitary. An electron microscope study. Am J Pathol 77:199-212, 1974.
11. Baes M, Allaerts W, Denef C. Evidence for functional communication between folliculo-stellate cells and hormone-secreting cells in perifused anterior pituitary cell aggregates. Endocrinology 120:685-691, 1987.
12. Lloyd RV, Mailloux J. Analysis of S100 protein positive folliculo-stellate cells in rat pituitary tissues. Am J Pathol 133:338-346, 1988.
13. Allaerts W, Jeucken PH, Debets R, Hoefakker S, Claassen E, Drexhage HA. Heterogeneity of pituitary folliculo-stellate cells: Implications for interleukin-6 production and accessary function in vitro. J Neuroendocrinol 9:43-53, 1997.
14. Vankelecom H, Matthys P, Van Damme J, Heremans H, Billiau A, Denef C. Immunocytochemical evidence that S-100 positive cells of the mouse anterior pituitary contain interleukin-6-immunoreactivity. J Histochem Cytochem 41:151-156, 1993.
15. Arzt E, Stalla GK. Cytokines: autocrine and paracrine roles in the anterior pituitary [Review]. Neuroimmunomodulation 3:28-34, 1996.
16. Jones TH, Daniels M, James RA, Justice SK, McCorkle R, Price A, et al. Production of bioactive and immunoreactive interleukin-6 (IL-6) and expression of IL-6 messenger ribonucleic acid by human pituitary adenomas. Clin Endocrin Metab. 78:180-187, 1994.
17. Spangelo BL, Judd AM, Isakson PC, MacLeod RM. Interleukin-1 stimulates interleukin-6 release from rat anterior pituitary cells in vitro. Endocrinology 128:2685-2692, 1991.
18. Spangelo BL, MacLeod RM, Isakson PC. Production of interleukin-6 by anterior pituitary cells in vitro. Endocrinology 12:582-586, 1990.
19. Geller DA, Billiar TR. Molecular biology of nitric oxide synthases [Review]. Cancer Metastasis Rev 17:7-23, 1998.
20. Wolf G. Nitric oxide and nitric oxide synthase: biology, pathology, localization [Review]. Histol Histopathol 12:251-261, 1997.
21. Lloyd RV, Jin L, Qian X, Zhang S, Scheithauer BW. Nitric oxide synthase in the human pituitary gland. Am J Pathol 146:86-94, 1995.
22. Wolff DJ, Datto GA. Identification and characterization of a calmodulin-dependent nitric oxide synthase from GH, pituitary cells. Biochem J 285:201-206, 1992.
23. Armour KE, Ralston SH. Estrogen upregulates endothelial constitutive nitric oxide synthase expression in human osteoblast-like cells. Endocrinology 139:799-802, 1998.
24. Ceccatelli S, Hulting AL, Zhang X, Gustafsson L, Villar M, Hokfelt T. Nitric oxide synthase in the rat anterior pituitary gland and the role of nitric oxide in regulation of luteinizing hormone secretion. Proc Natl Acad Sci USA 90:11,292-11,296, 1993.
25. Qian X, Jin L, Lloyd RV. Expression and regulation of transforming growth factor β-1 in cultured normal and neoplastic rat pituitary cells. Endocr Pathol 7:77-90, 1996.
26. Qian X, Jin L, Grande JP, Lloyd RV. Transforming growth factor-beta and p27 expression in pituitary cells. Endocrinology 137:3051-3060, 1996.
27. Magee T, Fuentes AM, Garban H, Rajavashisth T, Marquez D, Rodriguez JA, et al. Cloning of a novel neuronal nitric oxide symthase expression in penis and lower urinary tract. Biochem Biophys Res Commun 226:145-151, 1996.
28. Lloyd RV, Jin L. In situ hybridization analysis of chromogranin A and B mRNAs in neuroendocrine tumors with digoxigenin-labeled oligonucleotide probe cocktails. Diagn Mol Pathol 4:143-151, 1995.
29. Krown KA, Wang YF, Ho TW, Kelly PA, Walker AM. Prolactin isoform 2 as an autocrine growth factor for GH, cells. Endocrinology 131:595-602, 1992.
30. Duvilanski BH, Zambruno C, Seilicovich A, Pisera D, Lasaga M, Diaz MC, et al. Role of nitric oxide in control of prolactin release by the adenohypophysis. Proc Natl Acad Sci USA 92:170-174, 1995.