Generation of a histidine-tagged antibotulinum toxin antibody fragment in E. coli: Effects of post-induction temperature on yield and IMAC binding-affinity

Journal of Industrial Microbiology and Biotechnology

Volumn 21, Issue 6, 1998, Pages 275-282

  Bentley, W E ^a   Madurawe, R D ^a   Gill, R T ^a   Shiloach, M ^a   Chase, T E ^a   Pulliam Holoman, T R ^a   Valdes, J J ^b  

^a UNIVERSITY OF MARYLAND   (United States)

^b US Army Edgewood Research   (United States)

Author keywords

Botulinum toxin; E. coli fermentation; Fab antibody expression; Immobilized metal affinity chromatography (IMAC); Proteases; Temperature sensitivity

Indexed keywords

BOTULINUM ANTISERUM; BOTULINUM TOXIN; HISTIDINE; IMMUNOGLOBULIN F(AB) FRAGMENT; ISOPROPYL THIOGALACTOSIDE; PROTEINASE; RECOMBINANT ANTIBODY; SIGNAL PEPTIDE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; ENZYME ACTIVITY; ESCHERICHIA COLI; KINETICS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEIN TARGETING; TEMPERATURE SENSITIVITY;

EID: 0032458558     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.2900577     Document Type: Article

References (23)

1. 1 Better M, SL Bernhard, SP Lei, DM Fishwild, JA Lane, SF Carroll and AH Horwitz. 1993. Potent anti-CD5 ricin A chain immunoconjugates from bacterially produced Fab′ and F(ab′)2. Proc Natl Acad Sci USA 90: 457-461.
2. 2 Carter P, RF Kelley, ML Rodrigues, B Snedecor, M Covarrubias, MD Velligan, WLT Wong, AM Rowland, CE Kotts, ME Carver, M Yang, JH Bourell, M Shepard and D Henner. 1992. High level Escherichia coli expression and production of a bivalent humanized antibody fragment. Bio/Technology 10: 163-167.
3. 3 Emanuel P, T O'Brien, J Burans, BR DasGupta, JJ Valdes and M Eldefrawi. 1996. Directing antigen specificity towards botulinum neurotoxin with combinatorial phage display libraries. J Immunol Meth 193: 189-197.
4. 4 Georgiou G, ML Shuler and DB Wilson. 1988. Release of periplasmic enzymes and other physiological effects of β-lactamase overproduction in Escherichia coli by manipulation of culture conditions. J Ferment Bioeng 69: 159-165.
5. 5 Goff SA and AL Goldberg. 1985. Production of abnormal proteins in E. coli stimulates transcription of Ion and other heat shock dependent genes. Cell 41: 587-595.
6. 6 Gottesman S and M Maurizi. 1992. Regulation by proteolysis: energy-dependent proteases and their targets. Microbiol Rev 56: 592-621.
7. 7 Harcum SW and WE Bentley. 1993. Response dynamics of 26, 34, 39, 54, and 80 kDa proteases in induced cultures of recombinant Escherichia coli. Biotechnol Bioengin 42: 675-685.
8. 8 Horn U, W Strittmatter, A Krebber, U Knüpfer, M Kujau, R Wenderoth, K Müller, S Matzku, A Plückthun and D Riesenberg. 1996. High volumetric yields of functional dimeric miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions. Appl Microbiol Biotechnol 46: 524-532.
9. 9 Kitano K, S Fugimoto, M Nakao, T Watanabe and Y Nakao. 1987. Intracellular degradation of proteins in relation to their location in Escherichia coli cells. J Biotechnol 5: 77-86.
10. 10 Knappik A and A Plückthun. 1995. Engineered turns of a recombinant antibody improve its in vivo folding. Protein Eng 8: 81-89.
11. 11 Kosinski M, U Rinas and J Bailey. 1992. Proteolytic response to the expression of an abnormal β-galactosidase in Escherichia coli. Appl Microbiol Biotechnol 37: 335-341.
12. 12 Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227: 680-685.
13. 13 Lazdunski AM. 1989. Peptidases and proteases of Escherichia coli and Salmonella typhimurium. FEMS Microbiol Rev 63: 265-276.
14. 14 Maurizi M. 1992. Protease and protein degradation in Escherichia coli. Experientia 48: 178-199.
15. 15 Neidhardt F, R VanBogelan and V Vaughn. 1984. Genetics and regulation of heat shock proteins. Ann Rev Genet 18: 295-329.
16. 16 Pacaud M. 1982. Purification and characterization of two novel proteolytic enzymes in membranes of E. coli. J Biol Chem 257: 4333-4339.
17. 17 Pack P, M Kujau, V Schroeckh, U Knüpfer, R Wenderoth, D Riesenberg and A Plückthun. 1993. Improved bivalent miniantibodies, produced by high cell density fermentation of Escherichia coli. Bio/Technology 11: 1271-1277.
18. 18 Plückthun A. 1991. Antibody engineering: advances from the use of E. coli expression systems. Bio/Technology 9: 545-551.
19. 19 Porath J, J Carlsson, I Olsson and G Belfrage. 1975. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258: 598-599.
20. 20 Pulliam-Holoman T. 1996. PhD dissertation. University of Maryland, College Park.
21. 21 Ramirez DM and WE Bentley. 1995. Fed-batch feeding and induction policies that improve foreign protein synthesis and stability by avoiding stress responses. Biotechnol Bioengin 47: 596-609.
22. 22 Smith PK, RI Krohn, GT Hermanson, AK Mallia, FH Gartner, MD Provenzano, EK Fujimoto, NM Goeke, BJ Olson and DC Klenk. 1985. Measurement of proteins using bicinchoninic acid. Anal Biochem 150: 76-85.
23. v proteins and their fusion proteins. Methods. A companion to Meth Enzymol 2: 97-105.