An increase in apparent affinity for sucrose of mung bean sucrose synthase is caused by in vitro phosphorylation or directed mutagenesis of Ser

Plant and Cell Physiology

Volumn 39, Issue 12, 1998, Pages 1337-1341

  Nakai, Tomonori ^a   Konishi, Teruko ^a   Zhang, Xiu Qing ^b   Chollet, Raymond ^b   Tonouchi, Naoto ^c   Tsuchida, Takayasu ^c   Yoshinaga, Fumihiro ^c   Mori, Hitoshi ^d   Sakai, Fukumi ^a   Hayashi, Takahisa ^a  

^a KYOTO UNIVERSITY   (Japan)

^b UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^c Bio Polymer Research Co Ltd   (Japan)

^d NAGOYA UNIVERSITY   (Japan)

Author keywords

Directed mutagenesis; Protein phosphorylation; Sucrose synthase; Vigna radiata Wilczek

Indexed keywords

AMINO ACID SYNTHESIS; ENZYME ACTIVITY; MUTAGENESIS; NUTRIENT; PLANT RESIDUE; PROTEIN PHOSPHORYLATION; RECOMBINANT PROTEIN; SUCROSE SYNTHASE; SUCROSE SYNTHESIS; URIDINE DIPHOSPHATE;

ESCHERICHIA COLI; GLYCINE MAX; PHASEOLUS (ANGIOSPERM); VIGNA; VIGNA RADIATA; VIGNA RADIATA VAR. RADIATA;

EID: 0032455206     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.pcp.a029339     Document Type: Article

References (21)

1. Amor, Y., Haigier, C.H., Johnson, S., Wainscott, M. and Delmer, D.P. (1995) A membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. Proc. Natl. Acad. Sci. USA 92: 9353-9357.
2. Arai, M., Mori, H. and Imaseki, H. (1992) Expression of the gene for sucrose synthase during growth of mung bean seedlings. Plant Cell Physiol. 33: 503-506.
3. Ausubel, P.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (1988) Current protocols in molecular biology. Edited by Wiley, J. New York.
4. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
5. Chourey, P.S. and Nelson, O.E. (1976) The enzymatic deficiency conditioned by the shrunken-1 mutations in maize. Biochem. Genet. 14: 1041-1055.
6. Delmer, D.P. (1972) The purification and properties of sucrose synthetase from etiolated Phaseolus seedlings. J. Biol. Chem. 247: 3822-3828.
7. Elling, L., Grothus, M. and Kula, M.-R. (1993) Investigation of sucrose synthase from rice for the synthesis of various nucleotide sugars and saccharides. Glycobiology 3: 349-355.
8. Grimes, W.J., Jones, B.L. and Albersheim, P. (1970) Sucrose synthetase from Phaseolus aureus seedlings. J. Biol. Chem. 245: 187-197.
9. Huber, S.C., Huber, J.L., Liao, P.-C., Gage, D.A., McMichael, R.W., Jr., Chourey, P.S., Hannah, L.C. and Koch, K. (1996) Phosphorylation of serine-15 of maize leaf sucrose synthase. Occurrence in vivo and possible regulatory significance. Plant Physiol. 112: 793-802.
10. Kuster, H., Fruhling, M., Perlick, A.M. and Fuhler, A. (1993) The sucrose synthase gene is predominantly expressed in the root nodule tissue of Vicia faba. Mol. Plant-Microbe Interact. 6: 507-514.
11. Laemmli, U.K. (1970) Clevage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
12. Milner, Y. and Avigad, G. (1965) Thymidine diphosphate nucleotide as substrates in the sucrose synthetase reaction. Nature 206: 825.
13. Nakai, T., Tonouchi, N., Tsuchida, Y., Mori, T., Sakai, F. and Hayashi, T. (1997) Expression and characterization of sucrose synthase from mung bean seedlings in Escherichia coli. Biosci. Biotech. Biochem. 61: 1500-1503.
14. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467.
15. Shaw, J.R., Ferl, R.J., Baier, J., St. Clair, D., Carson, C., McCarty, D.R. and Hannah, L.C. (1994) Structural features of the maize sus1 gene and protein. Plant Physiol. 106: 1659-1665.
16. Slabnik, E., Frydman, R.B. and Cardini, C.E. (1968) Some properties of potato tuber UDPG:D-fructose-2-glucosyltransferase (E.C. 2.4.1.14) and UDPG:D-fructose-6-phosphate-2-glucosyItransferase (E.C. 2.4.1. 13). Plant Physiol. 43: 1063-1068.
17. Somogyi, M. (1952) Notes on sugar determination. J. Biol. Chem. 195: 19-23.
18. Tochikura, T., Kawai, H., Tobe, S., Kawaguchi, K., Osugi, M. and Ogata, K. (1968) Studies on microbial metabolisms of sugar nucleotides (I) UDP-glucose formation from 5′-UMP by yeasts. J. Ferment. Technol. 46: 957-969.
19. Winter, H., Huber, J.L. and Huber, S.C. (1997) Membrane association of sucrose synthase: changes during the graviresponse and possible control by protein phosphorylation. FEBS Lett. 420: 151-155.
20. 2+-dependent protein kinase. FEBS Lett. 410: 126-130.
21. Zhang, X.-Q., Verma, D.P.S., Patil, S., Arredondo-Peter, R., Miao, G.-H., Kuismanen, R., Klucas, R. V. and Chollet, R. (1997) Cloning of a full-length sucrose synthase cDNA from soybean (Glycine max) root nodules (Accession No. AFO30231) (PGR97-173). Plant Physiol. 115: 1729.