A reproducible procedure for primary culture and subsequent maintenance of multiple lines of human skin fibroblasts

Journal of the American Aging Association

Volumn 21, Issue 1, 1998, Pages 7-14

  Gibson, G E ^a   Tofel Grehl, B ^a   Scheffold, K ^a   Cristofalo, V J ^b   Blass, J P ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Alzheimer's disease; Fibroblasts

Indexed keywords

ALZHEIMER DISEASE; ARTICLE; CELL AGING; CELL POPULATION; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; REPRODUCIBILITY; SKIN FIBROBLAST;

EID: 0032454211     PISSN: 01619152     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11357-998-0002-z     Document Type: Article

References (19)

1. Alberts, B. Bray, D., Lewis, J., Raff, M, Roberts, K., Watson, J.D. Molecular Biology of the Cell. Second Edition. Garland Publishing. New York & London, 1989.
2. Cristofalo, VJ, and Charpentier, R. A standard procedure for cultivating human diploid fibroblast like cells to study cellular aging. J. Tissue Culture Methods.3&4:117-121,1980
3. Cristofalo, V G and Pignolo, RJ. Replicative senescence of human fibroblast-like cells in culture. Physiol. Reviews. 73:617-638, 1993.
4. Etcheberrigaray, R., Ito, E., Oka, K., Tofel-Grehl, B., Gibson, G.E. and Alkon, D.L Potassium channel dysfunction in fibroblasts identifies patients with Alzheimer's disease. Proc. Natl. Acad. Sci. (USA) 90:8209-8213, 1993.
5. Fohr, U.G., Gibson, G.E., Schafer, B.W. and Heizmann, C.W. Expression of intracellular calcium binding proteins in cultured skin fibroblasts from Alzheimer and normal aged donors. Biochim. Biophys. ACTA (Molecular and Cell Research) 1233:391-397, 1994.
6. Freshney, RI, Culture of Animal Cells. A Manual of Basic Technique. Second Edition.1987. by page 134 Alan R. Liss, NY, 1987.
7. Gauss-Muller, V, Kleinman, HK, Martin, GR, Schiffmann, E, Role of attachment factors and attractants in fibroblast chemotaxis. J. Lab.& Clin. Med. 96:1071-1080, 1980
8. Gibson, G., Martins, R., Blass, J. and Gandy S. Altered oxidation and signal transduction systems in fibroblasts from Alzheimer patients. IN: the Current Status of the Calcium Hypothesis of Brain Aging and Alzheimer's Disease. (ED W.E. Müller and W. Gispen) Life Science 59:477-490, 1996a.
9. Gibson, G.E., Zhang, H., Toral-Barza, L., Szolosi, S, and Tofel-Grehl, B. Calcium stores in cultured fibroblasts and their changes with Alzheimer's Disease. Biochim. Biophys. Acta 1316:71-77, 1996b.
10. Houck, JC, Sharma VK and Hayflick L. Functional failures of cultured human diploid fibroblasts after continued population doublings. Proc. Soc. Exp. Biol. Med.331-333, 1971.
11. Huang, H.-M. and Gibson, G.E. Altered β-adrenergic receptor stimulated cAMP formation in cultured skin fibroblasts from Alzheimer donors. J. Biol. Chem. 268:14616-14621, 1993.
12. Huang, H.-M., Lin, T.A., Sun, G.Y. and Gibson, G.E.. Increased inositol(1,4,5)trisphosphate accumulation correlates with an upregulation of bradykinin receptors in Alzheimer's disease. J. Neurochem. 64:761-766, 1995
13. Huang, H, Martins, R, Gandy S., Etcheberrigaray R, Ito E, Alkon, DL, Blass, JP and Gibson GE. The use of cultured fibroblasts in elucidating the pathophysiology and diagnosis of Alzheimer's disease. In "Calcium Hypothesis of Aging and Dementia" (Eds J.F. Disterhoft, W.H. Gispen, J. Traber, ZS Khachaturian) New York Academy of Sciences 747:225-244 1994
14. Huang, H.-M., Toral-Barza, L., Thaler, H., Tofel-Grehl, B. and Gibson, G.E.. Inositol phosphates and intracellular calcium after bradykinin stimulation in fibroblasts from young, normal aged and Alzheimer donors. Neurobiol. Aging 12:469-473, 1991.
15. 2+ mobilization is altered in fibroblasts from patients with Alzheimer disease. Proc. Natl. Acad. Sci. (USA) 91:534-539, 1994.
16. Phillips, H. IN: Tissue Culture: Methods and Applications, ed by Paul Kruse, JR. and MK Patterson, JR. Academic Press, NY, 1973.
17. Sheu, KFR, Cooper, AJL, Koike, K, Koike M, Lindsay JG, and Blass, JP. Abnormality of the alpha-ketoglutarate dehydrogenase complex in fibroblasts from familial Alzheimer's disease. Annals of Neurology. 35:312-318, 1994.
18. Stanbury, JB., Wyngaarden, JB, Fredrickson, DS, Goldstein, JL, Brown, MS. The Metabolic Basis of Inherited Disease Fifth Ed. McGraw Hill, New York, 1983.
19. Vestling, M, Adem, A, Racchi, M, Gibson, GE, Lannfelt L, and Cowburn RF. Differential regulation of adenylyl cyclase in fibroblasts from sporadic and familial Alzheimer's disease cases with PS1 and APP mutations. Neuroreport (in press), 1997.