Myc-epitope tagged proteins detected with the 9E10 antibody in immunofluorescence and immunoprecipitation assays but not in Western blot analysis

Biochemistry and Cell Biology

Volumn 76, Issue 1, 1998, Pages 125-128

  Fan, Huizhou ^a   Villegas, Cristy ^a   Chan, Arthur K ^a   Wright, Jim A ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

9E10; Gene expression; Immunofluorescence assay; Immunoprecipitation; Myc epitope; Recombinant protein; Western blot

Indexed keywords

EPITOPE; MONOCLONAL ANTIBODY; MYC PROTEIN;

ANIMAL CELL; ANTIGEN DETECTION; ANTIGEN RECOGNITION; ARTICLE; CELL STRAIN BHK; CONTROLLED STUDY; EXPRESSION VECTOR; GENETIC TRANSFECTION; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; NONHUMAN; PROTEIN EXPRESSION;

ANIMALIA;

EID: 0032450189     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/o98-003     Document Type: Article

References (11)

1. Chan, A.K., Litchfield, D.W., and Wright, J.A. 1993. Phosphorylation of ribonucleotide reductase R2 protein: in vivo and in vitro evidence of a role for p34cdc2 and CDK2 protein kinases. Biochemistry, 32: 12 835-12 840.
2. Engström, Y., and Rozell, B. 1988. Immunocytochemical evidence for the cytoplasmic localization and differential expression during the cell cycle of the M1 and M2 subunits of mammalian ribonucleotide reductase. EMBO J. 7: 1615-1620.
3. Evan, G.I., Lewis, G.K., Ramsay, G., and Bioshop, J.M. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616.
4. Fan, H., Villegas, C., and Wright, J.A. 1996a. Ribonucleotide reductase R2 component is a novel malignancy determinant that cooperates with activated oncogenes to determine transformation and malignant potential. Proc. Natl. Acad. Sci. U.S.A. 93: 14 036-14 040.
5. Fan, H., Villegas, C., Wright, J.A. 1996b. A link between ferritin gene expression and ribonucleotide reductase R2 protein, as demonstrated by retroviral vector mediated stable expression of R2 cDNA. FEBS Lett. 382: 145-148.
6. Fowlkes, D.M., Adams, M.D., Fowler, V.A., and Kay, B.K. 1992. Multipurpose vectors for peptide expression on the M13 viral surface. Biotechniques, 13: 422-428.
7. Graham, F.L., and van der Eb, A.H. 1973. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology, 52: 446-467.
8. Harlow, E.D., and Lane, D. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
9. Manstein, D.J., Schuster, H.P., Morandini, P., and Hunt, D.M. 1995. Cloning vectors for the production of proteins in Dictyostelium discoideum. Gene, 162: 129-134.
10. Zhong, G., Berry, J., and Brunham, R.C. 1994a. Antibody recognition of a neutralization epitope on the major outer membrane protein of Chlamydia trachomatis. Infect. Immun. 62: 1576-1583.
11. Zhong, G., Smith, G.P., Berry, J., and Brunham, R.C. 1994b. Conformational mimicry of a chlamydial neutralization epitope on filamentous phage. J. Biol. Chem. 269: 24 183-24 188.