Electroporation-mediated transformation of the ovine footrot pathogen Dichelobacter nodosus

FEMS Microbiology Letters

Volumn 169, Issue 2, 1998, Pages 383-389

  Kennan, Ruth M ^b   Billington, Stephen J ^a,b   Rood, Julian I ^b  

^a UNIVERSITY OF ARIZONA   (United States)

^b MONASH UNIVERSITY   (Australia)

Author keywords

Dichelobacter nodosus; Electroporation; Footrot; Homologous recombination; rRNA

Indexed keywords

ARTICLE; CONTROLLED STUDY; DICHELOBACTER NODOSUS; ELECTROPORATION; GENETIC RECOMBINATION; NONHUMAN; PATHOGENESIS; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHEEP DISEASE; VIRULENCE;

ANIMALS; ANTI-BACTERIAL AGENTS; BLOTTING, SOUTHERN; DICHELOBACTER NODOSUS; DRUG RESISTANCE, MICROBIAL; ELECTROPORATION; FOOT ROT; GENES, BACTERIAL; GRAM-NEGATIVE BACTERIAL INFECTIONS; PLASMIDS; POLYMERASE CHAIN REACTION; SHEEP; SHEEP DISEASES; TETRACYCLINE; TRANSFORMATION, BACTERIAL;

EID: 0032449875     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00513-8     Document Type: Article

References (15)

1. Stewart, D.J. (1989) Footrot of sheep. In: Footrot and Foot Abscess of Ruminants (Egerton, J.R., Yong, W.K. and Riffkin, G.G., Eds.) pp. 5-45. CRC Press, Boca Raton, FL.
2. Billington, S.J., Johnston, J.L. and Rood, J.I. (1996) Virulence regions and virulence factors of the ovine footrot pathogen, Dichelobacter nodosus. FEMS Microbiol. Lett. 145, 147-156.
3. Elleman, T.C. (1988) Pilins of Bacteroides nodosus: molecular basis of serotypic variation and relationships to other bacterial pilins. Microbiol. Rev. 52, 233-247.
4. Kortt, A.A., Riffkin, M.C., Focareta, A. and Stewart, D.J. (1993) Amino acid sequence of extracellular acidic protease V5 of Dichelobacter nodosus, the causative organism of ovine footrot. Biochem. Mol. Biol. Int. 29, 989-998.
5. Lilley, G.G., Stewart, D.J. and Kortt, A.A. (1992) Amino acid and DNA sequences of an extracellular basic protease of Dichelobacter nodosus show that it is a member of the subtilisin family of proteases. Eur. J. Biochem. 210, 13-21.
6. Katz, M.E., Strugnell, R.A. and Rood, J.I. (1992) Molecular characterization of a genomic region associated with virulence in Dichelobacter nodosus. Infect. Immun. 60, 4586-4592.
7. Katz, M.E., Wright, C.L., Gartside, T.S., Cheetham, B.F., Doidge, C.V., Moses, E.K. and Rood, J.I. (1994) Genetic organization of the duplicated vap region of the Dichelobacter nodosus genome. J. Bacteriol. 176, 2663-2669.
8. Cheetham, B.F., Tattersall, D.B., Bloomfield, G.A., Rood, J.I. and Katz, M.E. (1995) Identification of a bacteriophage-related integrase gene in a vap region of the genome of D. nodosus. Gene 162, 53-58.
9. Haring, V., Billington, S.J., Wright, C.L., Huggins, A.S., Katz, M.E. and Rood, J.I. (1995) Delineation of the virulence related locus vrl of Dichelobacter nodosus. Microbiology 141, 2081-2091.
10. La Fontaine, S. and Rood, J.I. (1996) Organization of ribosomal RNA genes from the footrot pathogen Dichelobacter nodosus. Microbiology 142, 889-899.
11. Billington, S.J., Sinistaj, M., Cheetham, B.F., Ayres, A., Moses, E.K., Katz, M.E. and Rood, J.I. (1996) Identification of a native Dichelobacter nodosus plasmid and implications for the evolution of the vap regions. Gene 172, 111-116.
12. La Fontaine, S., Egerton, J.R. and Rood, J.I. (1993) Detection of Dichelobacter nodosus using species-specific oligonucleotides as PCR primers. Vet. Microbiol. 35, 101-117.
13. Rood, J.I., Howarth, P.A., Haring, V., Billington, S.J., Yong, W.K., Liu, D., Palmer, M.A., Pitman, D.R., Links, I., Stewart, D.J. and Vaughan, J.A. (1996) Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus. Vet. Microbiol. 52, 127-141.
14. Anderson, B.J., Bills, M.M., Egerton, J.R. and Mattick, J.S. (1984) Cloning and expression in Escherichia coli of the gene encoding the structural subunit of Bacteroides nodosus fimbriae. J. Bacteriol. 160, 748-754.
15. Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503-517.