Network bursting by organotypic spinal slice cultures in the presence of bicuculline and/or strychnine is developmentally regulated

European Journal of Neuroscience

Volumn 10, Issue 9, 1998, Pages 2871-2879

  Ballerini, Laura ^a   Galante, Micaela ^a  

^a SISSA   (Italy)

Author keywords

GABA; Glycine; Interneurons; Oscillators; Rat; Spinal cord

Indexed keywords

BICUCULLINE; STRYCHNINE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; INTERNEURON; NERVE CELL CULTURE; NERVE CELL NETWORK; NERVOUS SYSTEM DEVELOPMENT; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; SPINAL CORD VENTRAL HORN; SPINAL GANGLION; SPINAL NERVE;

6-CYANO-7-NITROQUINOXALINE-2,3-DIONE; ANIMALS; BICUCULLINE; CELLS, CULTURED; COCULTURE TECHNIQUES; FETUS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; MEMBRANE POTENTIALS; NERVE NET; NEURONS; PIPERAZINES; RATS; SPINAL CORD; STRYCHNINE; SYNAPTIC TRANSMISSION; TETRODOTOXIN;

EID: 0032449496     PISSN: 0953816X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1460-9568.1998.00296.x     Document Type: Article

References (27)

1. Ballerini, L., Bracci, E. & Nistri, A. (1997) Pharmacological block of the electrogenic sodium pump disrupts rhythmic bursting induced by strychnine and bicuculline in the neonatal rat spinal cord. J. Neurophysiol., 77, 17-23.
2. Becker, C.M., Hoch, W. & Betz, H. (1988) Glycine receptor heterogeneity in rat spinal cord during postnatal development. EMBO J., 7, 3717-3726.
3. Bracci, E., Ballerini, L. & Nistri, A. (1996a) Spontaneous rhythmic bursts induced by pharmacological block of inhibition in lumbar motoneurons of the neonatal rat spinal cord. J. Neurophysiol., 75, 540-647.
4. Bracci, E., Ballerini, L. & Nistri, A. (1996b) Localization of rhythmogenic networks responsible for spontaneous bursts induced by strychnine and bicuculline in the rat isolated spinal cord. J. Neurosci., 16, 7063-7076.
5. Braschler, U.F., Iannone, A., Spenger, C., Streit, J. & Lüscher, H.R. (1989) A modified roller tube technique for organotypic cocultures of embryonic rat spinal cord, sensory ganglia and skeletal muscle. J. Neurosci. Meth., 29, 121-129.
6. Cazalets, J.R., Sqalli-Houssaini, Y. & Clarac, F. (1992) Activation of the central pattern generators for locomotion by serotonin and excitatory amino acids in neonatal rat. J. Physiol. Lon., 455, 187-204.
7. Cherubini, E., Gaiarsa, J.L. & Ben-Ari, Y. (1991) GABA: an excitatory transmitter in early post natal life. Trends Neurosci., 14, 515-519.
8. Clements, J.D. & Bekkers, J.M. (1997) Detection of spontaneous synaptic events with an optimally scaled template. Biophys. J., 73, 220-229.
9. Currie, S.N. & Stein, P.S.G. (1992) Glutamate antagonists applied to midbody spinal cord segments reduce the excitability of the fictive rostral scratch reflex in the turtle. Brain Res., 581, 91-100.
10. Gähwiler, B.H. (1981) Organotypic monolayer cultures of nervous tissue. J. Neurosci. Meth, 4, 329-342.
11. Gao, B.X. & Ziskind-Conhaim, L. (1995) Development of glycine- and GABA-gated currents in rat spinal motoneurons. J. Neurophysiol., 74, 113-121.
12. Grillner, S. (1985) Neurobiological bases of rhythmic motor acts in vertebrates. Sci. Wash. Dc, 228, 143-149.
13. Jakowec, M.W., Fox, A.J., Martin, L.J. & Kalb, R.G. (1995) Quantitative and qualitative changes in AMPA receptor expression during spinal cord development. Neuroscience, 67, 893-907.
14. Kudo, N. & Yamada, T. (1987) N-methyl-D,1-aspartate-induced locomotor activity in a spinal cord- hindlimb muscles preparation of the newborn rat studied in vitro. Neurosci. Lett., 75, 43-48.
15. Mandler, R.N., Schaffner, A.E., Novotny, E.A., Lange, G.D., Smith, S.V. & Barker, J.L. (1990) Electrical and chemical excitability appear one week before birth in the embryonic rat spinal cord. Brain Res., 522, 46-54.
16. Nishimaru, H., lizuka, M., Ozaki, S. & Kudo, N. (1996) Spontaneous motoneuronal activity mediated by glycine and GABA in the spinal cord of rat fetuses in vitro. J. Physiol., 497, 131-143.
17. O'Donovan, M.J. (1987) Developmental approaches to the analysis of vertebrate central pattern generators. J.Neurosci. Meth., 21, 275-286.
18. Reichling, D.B., Kyrozis, A., Wang, J. & MacDermott, A.B. (1994) Mechanisms of GABA and glycine depolarization-induced calcium transients in rat dorsal horn neurons. J. Physiol., 476, 411-421.
19. Rossignol., S. & Dubuc, R. (1994) Spinal pattern generation. Curr. Opin. Neurobiol., 4, 894-902.
20. Spenger, C., Braschler, U.F., Streit, J. & Lüscher, H.R. (1991) An organotypic spinal cord-dorsal root ganglion-skeletal muscle coculture of embryonic rat.I. The morphological correlates of the spinal reflex are. Eur. J. Neurosci., 3, 1037-1053.
21. Streit, J. (1993) Regular oscillations of synaptic activity in spinal networks in vitro. J.Neurophysiol., 70, 871-878.
22. Streit, J., Spenger, C. & Lüscher, H.R. (1991) An organotypic spinal cord-dorsal root ganglion-skeletal muscle coculture of embryonic rat. II. Functional evidence for the formation of spinal reflex arcs in vitro. Eur. J. Neurosci., 3, 1054-1068.
23. Takahashi, T. (1984) Inhibitory miniature synaptic potentials in rat motoneurons. Proc. R. Soc. Lond. B Biol. Sci., 221, 103-109.
24. Tölle, T.R., Berthele, A., Laurie, D.J., Seeburg, P.H. & Zieglgänsberger, W. (1995b) Cellular and subcellular distribution of NMDAR1 splice variant mRNA in the rat lumbar spinal cord. Eur. J. Neurosci., 7, 1235-1244.
25. Tölle, T.R., Berthele, A., Zieglgänsberger, W., Seeburg, P.H. & Wisden, W. (1995a) Flip and Flop variants of AMPA receptors in the rat lumbar spinal cord. Eur. J. Neurosci., 7, 1414-1419.
26. 2+ transients in embryonic rat dorsal horn neurons in culture. Eur. J. Neurosci., 6, 1275-1280.
27. Wu, W.L., Ziskind-Conhaim, L. & Sweet, M.A. (1992) Early development of glycine- and GABA-mediated synapses in rat spinal cord. J. Neurosci., 12, 3935-3945.