ELISA-based assay for scatchard analysis of ligand-receptor interactions

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 10, Issue 3, 1998, Pages 247-250

  Vieira, Amandio ^a  

^a NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

Author keywords

Binding assay; In vitro assay; Perforated cells; Scatchard analysis; Transferrin receptor

Indexed keywords

LIGAND; MEMBRANE RECEPTOR; TRANSFERRIN; TRANSFERRIN RECEPTOR;

CELL MEMBRANE; CENTRIFUGATION; ENZYME LINKED IMMUNOSORBENT ASSAY; LIGAND BINDING; QUANTITATIVE DIAGNOSIS; REVIEW; SCATCHARD PLOT;

EID: 0032449371     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02740845     Document Type: Review

References (6)

1. Smythe, E., Redelmeier, T. E., and Schmid, S. L. (1992) Receptor-mediated endocytosis in semi-intact cells. Methods Enzymol. 219, 223-234.
2. Davis, R. J. and Czech, M. P. (1986) Regulation of transferrin expression at the cell surface by insulin-like growth factors, epidermal growth factor and platelet-derived growth factor. EMBO J. 5, 653-658.
3. Wiley, H. S. and Kaplan, J. (1984) Epidermal growth factor rapidly induces a redistribution of transferrin receptor pools in human fibroblasts. Proc. Natl. Acad. Sci. USA 81, 7456-7460.
4. Ward, J. H., Kushner, J. P., and Kaplan, J. (1982) Transferrin receptors: Analysis of receptor properties and regulation. Biochem J. 208, 19-26.
5. Scatchard, G. (1949) The attraction of proteins for small molecules and ions. Ann. NY Acad. Sci. 51, 660-672.
6. Vieira, A., Elkin, R. G., and Kuchler, K. (1994) Western blotting and ligand blotting using enhanced chemiluminescence and radioiodine detection, in Cell Biology: A Laboratory Handbook (Celis, J., ed.), Academic, Orlando, FL, pp. 314-321.