Fluorescent in situ hybridization with 18S.26S rRNA genes reveal a novel site on chromosome 6 in addition to the satellited chromosome in pearl millet

Journal of Genetics and Breeding

Volumn 52, Issue 3, 1998, Pages 259-264

  Ahmad, F ^a   Comeau, A ^b   Collin, J ^c   St Pierre, C A ^c  

^a BRANDON UNIVERSITY   (Canada)

^b AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^c NONE

Author keywords

Genome mapping; Molecular cytogenetics; NORs; Pennisetum glaucum

Indexed keywords

CHROMOSOME ANALYSIS; FLUORESCENCE IN SITU HYBRIDIZATION; PEARL MILLET; PLANT BREEDING; PLANT GENETICS; PROTOPLAST;

EID: 0032448550     PISSN: 03949257     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. ABBO, S., MILLER, T.E., READER, S.M., DUNFORD, R.P. and KING, I.P., 1994. Detection of ribosomal DNA sites in lentil and chickpea by fluorescent in situ hybridization. Genome, 37: 713-716.
2. AHMAD, F., ACHARYA, S.N., MIR, Z. and MIR, P.S., 1998. Chromosomal localization and activity of rRNA genes on fenugreek (Trigonella foenum-graecum L.) chromosomes by fluorescence in situ hybridization and silver staining. Theor. Appl. Genet., In Press.
3. APPELS, R., GERLACH, W.L., DENNIS, E.S., SWIFT, H. and PEACOCK, W.J., 1980. Molecular and chromosomal organization of DNA sequences coding for the ribosomal RNAs in cereals. Chromosoma, 78: 293-311.
4. BAILEY, J.P., BENNETT, S.T., BENNETT, M.D. and STACE, C.A., 1993. Genomic in situ hybridization identifies parental chromosomes in the wild grass hybrid x Festulpia hubbardii. Heredity, 71: 413-420.
5. BURTON, G.W. and POWELL, J.B., 1968. Pearl millet breeding and cytogenetics. Adv. Agron., 20: 49-89.
6. BUSCH, W., HERMANN, R.G. and MARTIN, R., 1995. Refined physical mapping of the Sec-1 locus on the satellite of chromosome 1R of rye (Secale cereale). Genome, 38: 889-895.
7. CHEN, Q., CONNER, R.L. and LAROCHE, A., 1995. Identification of the parental chromosomes of the wheat alien amphiploid Agrotana by genomic in situ hybridization. Genome. 38: 1163-1169.
8. FERGUSON-SMITH, M.A., 1991. Putting the genetics back into cytogenetics. Am. J. Human Genet., 48: 179-182.
9. FLAVELL, R.B., 1980. The molecular characterization and organisation of plant chromosomal sequences. Ann. Rev. Plant Phys., 31: 569-596.
10. FOMINAYA, A., HUEROS, G., LOARCE, Y. and FERRER, E., 1995. Chromosomal distribution of a repeated DNA sequence from C-genome heterochromatin and the identification of a new ribosomal DNA locus in the Avena genus. Genome, 38: 548-557.
11. FRIEBE, B., ZHANG, W., RAUPP, J.W., GILL, B.S. and PORTER, D.R., 1995. Non-homoeologous wheat-rye chromosomal translocations conferring resistance to green bug. Euphytica, 84: 121-125.
12. GERLACH, W.L. and BEDBROOK, J.R., 1979. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Research, 7: 1869-1885.
13. GRIFFOR, M.C., VODKIN, L.O., SINGH, R.J. and HYMOWITZ, T., 1991. Fluorescent in situ hybridization to soybean metaphase chromosomes. Plant Molec. Biol., 17: 101-109.
14. HUBBELL, H.R., 1985. Silver staining as an indicator of active ribosomal genes. Stain Tech., 60: 285-294.
15. ISLAM-FARIDI, M.N. and MUJEEB-KAZI, A., 1995. Visualization of Secale cereale DNA in wheat germplasm by fluorescent in situ hybridization. Theor. Appl. Genet., 90: 595-600
16. JAUHAR, P.P., 1981. Cytogenetics of pearl millet. Adv. Agron., 34: 408-479.
17. KING, I.P., READER, S.M., PURDIE, K.A., ORFORD, S.E. and MILLER, T.E., 1994. A study of the effect of a homoeologous pairing promotor on chromosome pairing in wheat/rye hybrids using genomic in situ hybridization. Heredity. 72: 318-321.
18. KUMAR, K.A. and ANDREWS, D.J., 1993. Genetics of qualitative traits in pearl millet: a review. Crop Science, 33: 1-20.
19. LAVANIA, U.C., 1998. Fluorescence in situ hybridization in genome, chromosome and gene identification in plants. Curr. Sci., 74: 126-133.
20. LEITCH, I.J. and HESLOP-HARRISON, J.S., 1992. Physical mapping of the 18S-5.8S-26S rRNA genes in barley by in situ hybridization. Genome, 35: 1013-1018.
21. LEITCH, I.J., LEITCH, A.R. and HESLOP-HARRISON, J.S., 1991. Physical mapping of plant DNA sequences by simultaneous in situ hybridization of two differently labelled fluorescent probes. Genome, 34: 329-333.
22. LINDE-LAURSEN, I., IBSEN, E., VON BOTHMER, R. and GIESE, H., 1992. Physical localization of active and inactive rRNA loci in Hordeum marinum spp. gussoneanum (4x) by in situ hybridization. Genome, 35: 1032-1036.
23. MILLER, D.A., VEV, V.G. , TANTRAVAHI, R. and MILLER, J.O., 1976. Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp. Cell Res., 101: 235-243
24. MINOCHA, J.L., 1991, Pearl millet cytogenetics. In; GUPTA, P.K. and TSUCHIYA, T. eds., Chromosome engineering in plants: genetics, breeding, evolution. Part A, Elsevier Science Publishers, Netherlands, pp. 599-611.
25. MUJEEB-KAZI, A. and MIRANDA, J.L., 1985. Enhanced resolution of somatic chromosmes as an aid to identifying intergeneric hybrids among some Triticeae. Cytologia, 50: 701-709.
26. MUKAI, Y. and NAKAHARA, Y., 1993. Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome, 36: 489-494.
27. PANTULU, J.V. and KRISHNA RAO, M., 1982. Cytogenetics of pearl millet. Theor. Appl Genet., 66: 1-17.
28. PAROKONNY, A.S., KENTON, A.Y., GLEBA, Y.Y. and BENNETT, M.D., 1992. Genome organization in Nicotiana asymmetric somatic hybrids analysed by in situ hybridization. The Plant Journal, 2: 863-874.
29. ROGERS, S.O. and BENDICH, A.J., 1987. Ribosomal RNA gene in plants: variability in copy number and in the intergenic spacer. Plant Molec. Biol., 9: 509-520.
30. SCHWARZACHER, T., LEITCH, A.R., BENNETT, M.D. and HESLOP HARRISON, J.S., 1989. In situ localization of parental genomes in a wide hybrid. Ann. Bot., 64: 315-324.