The glycyl radical enzyme TdcE can replace pyruvate formate-lyase in glucose fermentation

Journal of Bacteriology

Volumn 180, Issue 14, 1998, Pages 3509-3516

  Sawers, Gary ^a   Heßlinger, Christian ^b,c   Muller, Nathalie ^b   Kaiser, Manuela ^b  

^a JOHN INNES CENTRE   (United Kingdom)

^b UNIVERSITY OF MUNICH   (Germany)

^c INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FORMATE ACETYLTRANSFERASE; GLUCOSE; GLYCINE DERIVATIVE; LYASE; PYRUVIC ACID; RADICAL; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIUM MUTANT; CLOSTRIDIUM BUTYRICUM; ENZYME SYNTHESIS; ESCHERICHIA COLI; FERMENTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM BUTYRICUM; ESCHERICHIA COLI; NEGIBACTERIA; STAPHYLOCOCCUS AUREUS;

EID: 0032448106     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.14.3509-3516.1998     Document Type: Article

References (43)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1987. Current protocols in molecular biology. Wiley Interscience, New York, N.Y.
2. Balch, W. E., and R. S. Wolfe. 1976. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl. Environ. Microbiol. 32:781-791.
3. Bastos, M., and E. Murphy. 1988. Transposon Tn554 encodes three products required for transposition. EMBO J. 7:2935-2941.
4. Bolivar, F., R. L. Rodriguez, P. J. Greene, M. C. Betlach, H. L. Heyneker, H. W. Boyer, J. H. Crosa, and S. Falkow. 1977. Cloning and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95-113.
5. Casadaban, M. J., and S. N. Cohen. 1979. Lactose genes fused to exogenous promoters in one step using Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc. Natl. Acad. Sci. USA 76:4530-4533.
6. Chattopadhyay, S., Y. Wu, and P. Datta. 1997. Involvement of Fnr and ArcA in anaerobic expression of the tdc operon of Escherichia coli. J. Bacteriol. 179:4868-4873.
7. Datta, P., T. J. Goss, J. R. Omnass, and R. V. Patil. 1987. Covalent structure of biodegradative threonine dehydratase of Escherichia coli: homology with other dehydratases. Proc. Natl. Acad. Sci. USA 84:393-397.
8. Ganduri, Y. L., S. R. Sadda, M. W. Datta, R. K. Jambukeswaran, and P. Datta. 1993. TdcA, a transcriptional activator of the tdcABC operon of Escherichia coli, is a member of the LysR family of proteins. Mol. Gen. Genet. 240:395-402.
9. Goss, T. J., H. P. Schweizer, and P. Datta. 1988. Molecular characterization of the tdc operon of Escherichia coli K-12. J. Bacteriol. 170:5352-5359.
10. Hagewood, B. T., Y. L. Ganduri, and P. Datta. 1994. Functional analysis of the tdcABC promoter of Escherichia coli: roles of TdcA and TdcR. J. Bacteriol. 176:6214-6220.
11. Heßlinger, C., S. A. Fairhurst, and G. Sawers. 1998. Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate. Mol. Microbiol. 27:477-492.
12. Heßlinger, C., and G. Sawers. The tdcE gene in Escherichia coli strain W3110 is separated from the rest of the tdc operon by insertion of IS5 elements. DNA Sequence, in press.
13. Hobert, E. H., and P. Datta. 1983. Synthesis of biodegradative threonine dehydratase in Escherichia coli: role of amino acids, electron acceptors, and certain intermediary metabolites. J. Bacteriol. 155:586-592.
14. Kaiser, M., and G. Sawers. 1994. Pyruvate formate-lyase is not essential for nitrate respiration by Escherichia coli. FEMS Microbiol. Lett. 117:163-168.
15. Kessler, D., and J. Knappe. 1996. Anaerobic dissimilation of pyruvate, p. 199-205. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
16. Kessler, D., W. Herth, and J. Knappe. 1992. Ultrastructure and pyruvate formate-lyase radical quenching property of the multienzymic AdhE protein of Escherichia coli. J. Biol. Chem. 267:18073-18079.
17. Knappe, J., and H. P. Blaschkowski. 1975. Pyruvate formate-lyase from Escherichia coli and its activation system. Methods Enzymol. 41:508-518.
18. Kohara, Y., K. Akiyama, and K. Isono. 1987. The physical map of the whole Escherichia coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495-508.
19. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
20. Leinfelder, W., K. Forchhammer, F. Zinoni, G. Sawers, M.-A. Mandrand-Berthelot, and A. Böck. 1988. Escherichia coli genes whose products are involved in selenium metabolism. J. Bacteriol. 170:540-546.
21. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
22. Lyons, L. B., and N. D. Zinder. 1972. The genetic map of the filamentous phage f1. Virology 49:45-60.
23. Mandrand-Berthelot, M.-A., M. K. Y. Wee, and B. A. Haddock. 1978. An improved method for identification and characterisation of mutants of Escherichia coli deficient in formate dehydrogenase activity. FEMS Microbiol. Lett. 4:37-40.
24. Murphy, E., L. Huwyler, and M. Bastos. 1985. Transposon Tn554: complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants. EMBO J. 4:3357-3365.
25. Murphy, E., and S. Löfdahl. 1984. Transposition of Tn554 does not generate a target duplication. Nature 307:292-294.
26. Murphy, E., E. Reinheimer, and L. Huwyler. 1991. Mutational analysis of att554, the target of the site-specific transposon Tn554. Plasmid 26:20-29.
27. Phillips, A. T., and W. A. Wood. 1965. The mechanism of action of 5′-adenylic acid-activated threonine dehydratase. J. Biol. Chem. 240:4703-4709.
28. Rossmann, R., G. Sawers, and A. Böck. 1991. Mechanism of regulation of the formate-hydrogen lyase pathway by oxygen, nitrate, and pH: definition of the formate regulon. Mol. Microbiol. 5:2807-2814.
29. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
30. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
31. Sauter, M., and R. G. Sawers. 1990. Transcriptional analysis of the gene encoding pyruvate formate-lyase-activating enzyme of Escherichia coli. Mol. Microbiol. 4:355-363.
32. Sawers, G., and A. Böck. 1988. Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12. J. Bacteriol. 170:5330-5336.
33. Sawers, G., and A. Böck. 1989. Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression. J. Bacteriol. 171:2485-2498.
34. Tabor, S., and C. C. Richardson. 1985. A bacteriophage T7 polymerase/ promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
35. Thauer, R. K., F. H. Kirschniawy, and K. A. Jungermann. 1972. Properties and function of the pyruvate formate-lyase reaction in clostridia. Eur. J. Biochem. 27:282-290.
36. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
37. Wagner, A. F. V., M. Frey, F. A. Neugebauer, W. Schäfer, and J. Knappe. 1992. The free radical in pyruvate formate-lyase is located on glycine-734. Proc. Natl. Acad. Sci. USA 89:996-1000.
38. Weidner, G., and G. Sawers. 1996. Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum. J. Bacteriol. 178:2440-2444.
39. Wu, Y., and P. Datta. 1992. Integration host factor is required for positive regulation of the tdc operon of Escherichia coli. J. Bacteriol. 174:233-240.
40. Wu, Y., and P. Datta. 1995. Influence of DNA topology on expression of the tdc operon in Escherichia coli K-12. Mol. Gen. Genet. 247:764-767.
41. Wu, Y., R. V. Patil, and P. Datta. 1992. Catabolite gene activator protein and integration host factor act in concert to regulate tdc operon expression in Escherichia coli. J. Bacteriol. 174:6918-6927.
42. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
43. Zinoni, F., A. Birkmann, T. C. Stadtman, and A. Böck. 1986. Nucleotide sequence and expression of the selenocysteine-containing polypeptide of formate dehydrogenase (formate hydrogen-lyase-linked) from Escherichia coli. Proc. Natl. Acad. Sci. USA 83:4650-4654.