Applicability of SSCP analysis for MHC genotyping: Fingerprinting of Ovar-DRB1 exon 2 alleles from Finnish and Russian breeds

Animal Genetics

Volumn 29, Issue 6, 1998, Pages 453-455

  Kostia, S ^a   Kantanen, J ^b   Kolkkala, M ^a   Varvio, S L ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b FINNISH FOREST RESEARCH INSTITUTE   (Finland)

Author keywords

Allele fingerprinting; Genotyping; MhcOvar; SSCP

Indexed keywords

ALLELE; ANIMAL BREEDING; EXON; MAJOR HISTOCOMPATIBILITY COMPLEX; SHEEP; SINGLE STRAND CONFORMATION POLYMORPHISM;

ALLELES; ANIMALS; DNA FINGERPRINTING; EXONS; GENOTYPE; HISTOCOMPATIBILITY ANTIGENS; MAJOR HISTOCOMPATIBILITY COMPLEX; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SHEEP; VARIATION (GENETICS);

ANIMALIA; MAMMALIA; OVIS ARIES;

EID: 0032444301     PISSN: 02689146     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2052.1998.296376.x     Document Type: Article

References (5)

1. Girman D.J. (1996) The use of PCR-based single-stranded conformation polymorphism analysis (PCR-SSCP) in conservation genetics. In: Molecular Genetic Approaches in Conservation (ed. by T. B. Smith & R. K. Wayne), pp. 167-82. Oxford University Press, Oxford.
2. Maijala K. (1997) Genetic aspects of domestication, common breeds and their origin. In: The Genetics of Sheep (ed. by L. Piper & A. Ruvinsky), pp. 13-49. CAB International, Wallinford, Oxon.
3. Orita M., Suzuki Y., Sekiya T. & Hayashi K. (1989) Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5, 874-9.
4. Schwaiger F.-W., Maddox J., Ballingal K. et al. (1996) The ovine major histocompatibility complex. In: The Major Histocompatibilty Complex of Domesticated Animals (ed. by L. B. Schook & S. J. Lamont), pp. 121-76. CRC press, Boca Raton, FL.
5. Sigurdardóttir S., Borsch C., Gustafsson K. & Andersson L. (1991) Cloning and sequence analysis of 14 DRB alleles of the bovine major histocompatibility complex by using the polymerase chain reaction. Animal Genetics 22, 199-209.