Purification and characterization of a serine protease in erythrocyte cytosol that is adherent to oxidized membranes and preferentially degrades proteins modified by oxidation and glycation

Journal of Biochemistry

Volumn 124, Issue 6, 1998, Pages 1077-1085

  Fujino, Tomofumi ^a   Tada, Tomohiro ^a   Beppu, Masatoshi ^a   Kikugawa, Kiyomi ^a  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

Author keywords

Diisopropyl fluorophosphate; Erythrocyte serine protease; Glycation; Oxidation; Protein degradation

Indexed keywords

BOVINE SERUM ALBUMIN; DODECYL SULFATE SODIUM; DYFLOS; ERYTHROCYTE ENZYME; HEMOGLOBIN; MEMBRANE ENZYME; SEPHADEX; SERINE PROTEINASE; TRITIUM;

ARTICLE; CYTOSOL; ENZYME PURIFICATION; ERYTHROCYTE MEMBRANE; FLUOROGRAPHY; GLYCATION; HUMAN; HUMAN CELL; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION;

BOVINAE; TRITIUM;

EID: 0032444266     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022224     Document Type: Article

References (31)

1. Davies, K.J.A. (1986) Intracellular proteolytic systems may function as secondary antioxidant defenses: An hypothesis. Free Rad. Biol. Med. 2, 155-173
2. Pacifici, R.E., Salo, D.C., and Davies, K.J.A. (1989) Macroxyproteinase (M.O.P.): A 670 kDa proteinase complex that degrades oxidatively denatured proteins in red blood cells. Free Rad. Biol. Med. 7, 521-536
3. Sacchetta, P., Battista, P., Santarone, S., and Cola, D.D. (1990) Purification of human erythrocyte proteolytic enzyme responsible for degradation of oxidant-damaged hemoglobin. Evidence for identifying as a member of the multicatalytic proteinase family. Biochim. Biophys. Acta 1037, 337-343
4. Fagan, J.M. and Waxman, L. (1991) Purification of a protease in red blood cells that degrades oxidatively damaged haemoglobin. Biochem. J. 277, 779-786
5. Beppu, M., Inoue, M., Ishikawa, T., and Kikugawa, K. (1994) Presence of membrane-bound proteinases that preferentially degrade oxidatively damaged erythrocyte membrane proteins as secondary antioxidant defense. Biochim. Biophys. Acta 1196, 81-87
6. Fujino, T., Ishikawa, T., Inoue, M., Beppu, M., and Kikugawa, K. (1998) Characterization of membrane-bound serine protease related to degradation of oxidatively damaged erythrocyte membrane proteins. Biochim. Biophys. Acta 1374, 47-55
7. Beppu, M., Mizukami, A., Nagoya, M., and Kikugawa, K. (1990) Binding of anti-band 3 autoantibody to oxidatively damaged erythrocytes. J. Biol Chem. 265, 3226-3233
8. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
9. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
10. Davies, K.J.A. (1987) Protein damage and degradation by oxygen radicals. J. Biol. Chem. 262, 9895-9901
11. Johnstone, A. and Thorpe, R., eds. (1982) Immunochemistry in Practice, p. 2, Blackwell Scientific Publications, Boston
12. Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354
13. 125I-containing acylating agent. Biochem. J. 133, 529-538
14. 2+-sensitive thiol protease (calpain I) and its specific inhibitor protein (calpastatin). J. Biochem. 90, 1809-1816
15. Yamamoto, K., Takeda, M., Yamamoto, H., Tatsumi, M., and Kato, Y. (1985) Human erythrocyte membrane acid proteinase (EMAP): Sidendness and relation to cathepsin D. J. Biochem. 97, 821-830
16. Yamamoto, K. and Marchesi, V.T. (1984) Purification and characterization of acid proteinase from human erythrocyte membranes. Biochim. Biophys. Acta 790, 208-218
17. Pontremoli, S., Salamino, F., Sparatore, B., Melloni, E., Morelli, A., Benatti, U., and Flora, A.D. (1979) Isolation and partial characterization of three acidic proteinases in erythrocyte membranes. Biochem. J. 181, 559-568
18. Kinoshita, M., Hanakubo, T., Fukui, I., Murachi, T., and Toyohara, H. (1990) Significant amount of multicatalytic proteinase identified on membrane from human erythrocyte. J. Biochem. 107, 440-444
19. Davies, K.J.A., Delsignore, M.E., and Lin, S.W. (1987) Protein damage and degradation by oxygen radicals II. Modification of amino acids. J. Biol. Chem. 262, 9902-9907
20. Davies, K.J.A. and Delsignore, M.E. (1987) Protein damage and degradation by oxygen radicals III. Modification of secondary and tertiary structure. J. Biol. Chem. 262, 9908-9913
21. Cervera, J. and Levine, R.T. (1988) Modulation of the hydrophobicity of glutamine synthetase by mixed-function oxidation. FASEB. J. 2, 2591-2595
22. Pacifici, R.E., Kono, Y., and Davies, K.J.A. (1993) Hydrophobicity as the signal for selective degradation of hydroxy radical-modified hemoglobin by the multicatalytic proteinase comples, proteasome. J. Biol. Chem. 268, 15405-15411
23. Goldberg, A.L. and Boches, F.S. (1982) Oxidized proteins in erythrocytes are rapidly degraded by the adenosine triphosphate-dependent proteolytic system. Science 215, 1107-1109
24. Fagan, J.W.M., Waxman, L., and Goldberg, A.L. (1986) Red blood cells contain a pathway for the degradation of oxidant-damaged hemoglobin that does not require ATP or ubiquitin. J. Biol. Chem. 261, 5705-5713
25. Davies, K.J.A. and Goldberg, A.L. (1987) Oxygen radicals stimulate intracelluar proteolysis and lipid peroxidation by independent mechanisms in erythrocytes. J. Biol. Chem. 262, 8220-8226
26. Davies, K.J.A. and Goldberg, A.L. (1987) Proteins damaged by oxygen redicals are rapidly degraded in extracts of red blood cells. J. Biol. Chem. 262, 8227-8234
27. Dean, R.T. and Pollak, J.K. (1985) Endogenous free radical generation may influence proteolysis in mitochondria. Biochem. Biophys. Res. Commun. 126, 1082-1089
28. Wolff, S.P., Garner, A., and Dean, R.T. (1986) Free radicals, lipids and protein degradation. Trends Biol. Sci. 11, 27-31
29. Levine, R.L., Oliver, C.N., Fulks, R.M., and Stadtman, E.R. (1981) Turnover of bacterial glutamine synthetase: Oxidative inactivation precedes proteolysis. Proc. Natl. Acad. Sci. USA 78, 2120-2124
30. Davies, K.J.A. and Lin, S.W. (1988) Degradation of oxidatively denatured proteins in Escherichia coli. Free Rad. Biol. Med. 5, 215-223
31. Davies, K.J.A. and Lin, S.W. (1988) Oxidatively denatured proteins are degraded by an ATP-independent proteolytic pathway in Escherichia coli. Free Rad. Biol. Med. 5, 225-236