Adenoviral gene transfer into the normal and injured spinal cord: Enhanced transgene stability by combined administration of temperature-sensitive virus and transient immune blockade

Gene Therapy

Volumn 5, Issue 12, 1998, Pages 1612-1621

  Romero, M I ^a   Smith, G M ^a,b  

^a UNIVERSITY OF TEXAS AT DALLAS   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Adenovirus; Gene therapy; Immunosuppression; Spinal cord; T lymphocytes; Thermal nociception

Indexed keywords

BETA GALACTOSIDASE; CD4 ANTIGEN; CD45 ANTIGEN; CD8 ANTIGEN; LYMPHOCYTE RECEPTOR;

ADENOVIRUS; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL FUNCTION; CONTROLLED STUDY; CYTOPATHOLOGY; DORSAL ROOT; EXPRESSION VECTOR; FEMALE; GENE THERAPY; GENE TRANSFER; GENETIC STABILITY; GENETIC TRANSFECTION; IMMUNOGENICITY; IMMUNOSUPPRESSIVE TREATMENT; MACROPHAGE; MICROGLIA; NERVE CELL; NOCICEPTION; NONHUMAN; PRIORITY JOURNAL; RAT; RECEPTOR BLOCKING; SPINAL CORD INJURY; STAINING; TEMPERATURE; TRANSGENE;

ADENOVIRIDAE; ANIMALIA;

EID: 0032440741     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300774     Document Type: Article

References (8)

1. Blömer U et al Applications of gene therapy to the CNS. Hum Mol Gen 1996; 5: 1397-1404.
2. Karpati G, Lochmuller H, Nalbantoglu J, Durham H. The principles of gene therapy for the nervous system. Trends Neurosci 1996; 19: 49-54.
3. Zlokovic BV, Apuzzo MLJ. Cellular and molecular neurosurgery: pathways from concept to reality - part II: vector systems and delivery methodologies for gene therapy of the central nervous system. Neurosurgery 1997; 40: 805-812.
4. Peel AL et al. Efficient transduction of green fluorescent protein in spinal cord neurons using adeno-associated virus vectors containing cell type-specific promoters. Gene Therapy 1997; 4:16-24.
5. Liu Y et al. Application of recombinant adenovirus for in vivo gene delivery to the spinal cord. Brain Res 1997; 768: 19-29.
6. Romero MI, Smith GM. Adenoviral gene transfer of neurotrophins and cell adhesion molecules into the adult rat spinal cord and their effect on axonal regeneration. Soc Neurosci 1997; 23: (Suppl. 2): 1450 (Abstr. 573.3).
7. Hermens WTJMC et al. Transient gene transfer to neurons and glia: analysis of adenoviral vector performance in the CNS and PNS. J Neurosci Meth 1997; 71: 85-98.
8. Byrnes AP, Rusby JE, Wood MJA, Charlton MM. Adenovirus gene transfer causes inflammation in the brain. Neuroscience 1995; 66: 1015-1024.