PCR-based strategy for genotyping mice and ES cells harboring LoxP sites

BioTechniques

Volumn 25, Issue 6, 1998, Pages 968-972

  Mantamadiotis, Theo ^a   Taraviras, Stavros ^a   Tronche, François ^a   Schütz, Günther ^a  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RECOMBINASE; TRANSCRIPTION FACTOR;

ALLELE; ANIMAL CELL; ARTICLE; EMBRYO; GENE MUTATION; GENETIC RECOMBINATION; GENOTYPE; KNOCKOUT GENE; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION;

ANIMALIA;

EID: 0032440374     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98256bm07     Document Type: Article

References (5)

1. Betz, U.A., C.A. Vosshenrich, K. Rajewsky and W. Muller. 1996. Bypass of lethality with mosaic mice generated by Cre-loxP-mediated recombination. Curr. Biol. 6:1307-1316.
2. Bronson, S.K. and O. Smithies. 1994. Altering mice by homologous recombination using embryonic stem cells. J. Biol. Chem. 269:27155-27158.
3. Chambers, C.A. 1994. TKO'ed: lox, stock and barrel. Bioessays 16:865-868.
4. Terry, R.W., L. Kwee, H.S. Baldwin and M.A. Labow. 1997. Cre-mediated generation of a VCAM-1 null allele in transgenic mice. Transgenic Res. 6:349-356.
5. Tsien, J.Z., D.F. Chen, D. Gerber, C. Tom, E.H. Mercer, D.J. Andersen, M. Mayford, E.R. Kandel and S. Tonegawa. 1996. Subregion- and cell type-restricted gene knockout in mouse brain. Cell 87:1317-1326.