An assessment of the accuracy and reproducibility of the most probable number (MPN) technique in estimating numbers of nutrient stressed diatoms in sediment samples

Journal of Experimental Marine Biology and Ecology

Volumn 231, Issue 1, 1998, Pages 21-30

  Harris, A S D ^a,b   Jones, K J ^b   Lewis, J ^a  

^a UNIVERSITY OF WESTMINSTER   (United Kingdom)

^b SCOTTISH MARINE INSTITUTE   (United Kingdom)

Author keywords

Algal culture; Diatoms; Most probable number (MPN); Sediments

Indexed keywords

DRINKING WATER;

DIATOM; ESTIMATION METHOD; RELATIVE ABUNDANCE; SEDIMENT;

ACCURACY; ALGA; ARTICLE; CELL VIABILITY; DIATOM; NUTRIENT; PLANKTON; REPRODUCIBILITY; SAMPLING; SEDIMENT; TECHNIQUE;

DITYLUM BRIGHTWELLII; SKELETONEMA COSTATUM;

EID: 0032438902     PISSN: 00220981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-0981(98)00061-6     Document Type: Article

References (31)

1. Alexander, M., 1982. Most probable number method for microbial populations. In: Page, A.L. (Ed.), Methods of soil analysis. Part 2. Chemical and Microbiological Properties, 2nd ed. American Society of Agronomy, Inc., Madison, Wisconsin, USA, pp. 815-820.
2. An K.H., Lassus P., Maggi P., Bardouil M., Truquet P. Dinoflagellate cyst changes and winter environmental conditions in Vilaine Bay, Southern Brittany (France). Bot. Mar. 35:1992;61-67.
3. Anon., 1982. The bacteriological examination of drinking water supplies 1982. Reports on Public Health and Medical Subjects, No. 71. Her Majesty's Stationary Office, London.
4. Ballantine D. Comparison of the different methods of estimating nanoplankton. J. Mar. Biol. Assoc. UK. 32:1953;129-147.
5. Beliaeff B., Mary J.-Y. The 'most probable number' estimate and its confidence limits. Water Res. 5:1993;799-805.
6. Bernhard M., Rampi L., Zattera A. A phytoplankton component not considered by the Utermöhl method (Pubblicazioni della Stazione Zoologica di Napoli I). Mar. Ecol. 35:1967;170-214.
7. Boleyn B.J. Silica availability and the formation of dysplatic cells in cultures of Ditylum brightwellii (West) Grunow. Arch. Microbiol. 81:1972;169-177.
8. Cochran W.G. Estimation of bacterial densities by means of the 'most probable number'. Biometrics. 6:1950;105-116.
9. Colwell, R.R., 1979. Enumeration of specific populations by the most probable number (MPN) method. In: Costerton J.W., Colwell, R.R. (Eds.), Native Aquatic Bacteria: Enumeration, Activity and Ecology. ASTM STP 695, pp. 56-61.
10. Dodson, A.N., Thomas, W.H., 1978. Reverse filtration. In: Sournia, A. (Ed.), Phytoplankton Manual. Monographs on Oceanographic Methodology 6. UNESCO, Paris, pp. 104-107.
11. Erard-Le Denn, E., Boulay, V., 1995. Resting cysts of Alexandrium minutum in marine sediment: quantification by three different techniques. In: Lassus, P., Arzul, G., Erard-Le Denn, E., Gentien, P., Marcaillou-Le Baut, C. (Eds.), Proceedings of the Sixth International Conference on Toxic Marine Phytoplankton, Nantes, 1993, Lavoisier, Paris, pp. 725-730.
12. Erard-Le Denn, E., Desbruyeres, E., Olu, K., 1993. Alexandrium minutum: resting cyst distribution in the sediments collected along the Brittany coast, France. In: Smayda, T.J., Shimizu, Y. (Eds.), Toxic Phytoplankton Blooms in the Sea. Elsevier Science Publishers, Amsterdam, pp. 109-114.
13. French F.W., Hargraves P.E. Physiological characteristics of plankton diatom resting spores. Mar. Biol. Lett. 1:1980;185-195.
14. Furuya K., Marumo R. The structure of the phytoplankton community in the subsurface chlorophyll maxima in the western North Pacific Ocean. J. Plankton Res. 5:1983;393-406.
15. Gross F. The development of isolated resting spores into auxospores in Ditylum brightwelli (West.). J. Mar. Biol. Assoc. UK. 24:1940;375-380.
16. Guillard, R.R.L., 1975. Culture of phytoplankton for feeding marine invertebrates. In: Smith, W.L., Charley, M.H. (Eds.), Culture of Marine Invertebrate Animals. Plenum, New York, pp. 29-60.
17. Halvorson H.O., Ziegler N.R. Application of statistics to problems in bacteriology. J. Bacteriol. 25:1933;101-121.
18. Hargraves, P.E., 1982. Resting spore formation in the marine diatom Ditylum brightwellii (West) Grun. ex V.H. In: Mann, D.G. (Ed.), Proceedings of the Seventh International Diatom Symposium, Koeltz, Koenigstein, pp. 33-46.
19. Hargraves, P.E., French, F.W., 1983. Diatom resting spores: significance and strategies. In: Fryxell, G.A. (Ed.), Survival Strategies of the Algae. Cambridge University Press, Cambridge, pp. 49-68.
20. Harris, A.S.D., 1995. The ecological role of diatom resting stages in coastal waters. Ph.D. Dissertation. University of Westminster, London.
21. Imai I., Itoh K. Annual life cycle of Chattonella spp., causative flagellates of noxious red tides in the Inland Sea of Japan. Mar. Biol. 94:1987;287-292.
22. Imai I., Itoh K., Anraku M. Extinction dilution method for enumeration of dormant cells of red tide organisms in marine sediments. Bull. Plankton Soc. Jpn. 31:1984;123-124.
23. Imai I., Itakura S., Itoh K. Distributions of diatom resting cells in the sediments of Harima-Nada and northern Hiroshima Bay, the Seto Inland Sea, Japan. Bull. Coast. Oceanogr. 28:1990;75-84.
24. Itakura S., Imai I., Itoh K. Morphology and rejuvenation of Skeletonema costatum (Bacillariophyceae) resting cells from the bottom sediments of Hiroshima Bay, the Seto Inland Sea, Japan. Bull. Plankton Soc. Jpn. 38:1992;135-145.
25. Jochem F.J. On the seasonal occurrence of autotrophic naked nanoflagellates in Kiel Bight, western Baltic. Estuarine Coast. Shelf Sci. 31:1990;189-202.
26. Larrazabal M.E., Lassus P., Maggi P., Bardouil M. Kystes modernes de dinoflagellés en baie de Vilaine-Bretagne Sud (France). Cryptogamie Algol. 11:1990;171-185.
27. Kranck K., Milligan T.G. Macroflocs from diatoms: in situ photography of particles in Bedford Basin, Nova Scotia. Mar. Ecol. Progr. Ser. 44:1988;183-189.
28. de Man J.C. The probability of most probable numbers. Eur. J. Appl. Microbiol. 1:1975;67-78.
29. Massana R., Güde H. Comparison between three methods for determining flagellate abundance in natural waters. Ophelia. 33:1991;197-203.
30. McAlice B.J. Phytoplankton sampling with the Sedgwick-Rafter cell. Limnol. Oceanogr. 16:1971;19-28.
31. Throndsen, J., 1978. The dilution-culture method. In: Sournia, A. (Ed.), Phytoplankton Manual. Monographs on Oceanographic Methodology 6. UNESCO, Paris, pp. 218-224.