Inactivation and conformational changes of fatty acid synthase from chicken liver during unfolding by sodium dodecyl sulfate

International Journal of Biochemistry and Cell Biology

Volumn 30, Issue 12, 1998, Pages 1319-1330

  Van, Shi ^a,b   Luo, Wei ^a   Tian, Wei Xi ^a   Zhang, Tong ^a   Zhou, Hai Meng ^a  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b TSINGHUA UNIVERSITY   (China)

Author keywords

Chicken liver; Conformational changes; Fatty acid synthase; Inactivation; Sodium dodecyl sulfate

Indexed keywords

FATTY ACID SYNTHASE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CHICKEN; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DENATURATION; ENZYME ACTIVITY; ENZYME INACTIVATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION; PROTEIN STRUCTURE;

ANIMALS; CHICKENS; FATTY ACID SYNTHETASE COMPLEX; KINETICS; LIVER; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; SODIUM DODECYL SULFATE; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY, ULTRAVIOLET; SURFACE-ACTIVE AGENTS;

GALLUS GALLUS;

EID: 0032437002     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(98)00101-0     Document Type: Article

References (20)

1. S. Smith, The animal fatty acid synthase: one gene, one polypeptide, seven enzymes, FASEB J. 8 (1994) 1248-1259.
2. R.Y. Hsu, S.L. Yun, Stabilization and physicochemical properties of the fatty acid synthetase of chicken liver, Biochemistry 9 (1970) 239-245.
3. C.L. Tang, R.Y. Hsu, Fatty acid synthase of chicken liver. Reversible dissociation into two nonidentical subcomplexes of similar size, J. Biol. Chem. 247 (1974) 2689-2698.
4. A. Witkowski, V.S. Rangan, Z.I. Randhawa, C.M. Amy, S. Smith, Structural organization of the multifunctional animal fatty acid synthase, Eur. J. Biochem. 198 (1991) 571-579.
5. S.J. Liang, Y.Z. Lin, J.M. Zhou, C.L. Tsou, P.Q. Wu, Z.M. Zhou, Dissociation and aggregation of D-glyceral-dehyde-3-phosphate dehydrogenase during denaturation by guanidine hydrochloride, Biochim. Biophys. Acta 1038 (1990) 240-246.
6. 4 during denaturation by guanidinium chloride, Biochem. J. 277 (1991) 207-211.
7. L.Y. Chen, M. Tian, J.S. Du, M. Ju, The changes of circular dichroism and fluorescence spectra, and the comparison with inactivation rates of angiotensin converting enzyme in guanidine solutions, Biochim. Biophys. Acta 1039 (1990) 61-66.
8. H.R. Wang, T. Zhang, H.M. Zhou, Comparison of inactivation and conformational changes of aminoacylase during guanidium chloride denaturation, Biochim. Biophys. Acta 1248 (1995) 97-106.
9. Q.X. Chen, W. Zhang, W.Z. Zhang, S.X. Yan, T. Zhang, H.M. Zhou, Comparison of inactivation and unfolding of green crab (Scylla serrata) alkaline phosphatase, J. Protein Chem. 15 (4) (1996) 359-365.
10. C.L. Tsou, Location of the active sites of some enzymes in limited and flexible molecular regions, Trends Biochem. Sci. 11 (10) (1986) 427-429.
11. C.L. Tsou, Conformational flexibility of enzyme active sites, Science 262 (1993) 380-381.
12. W.X. Tian, R.Y. Hsu, Y.S. Wang, Studies on the reactivity of the essential sulfhydryl groups as a conformational probe for the fatty acid synthetase of chicken liver, J. Biol. Chem. 260 (20) (1985) 11375-11387.
13. S. Kumar, J.A. Dorsey, R.A. Muesing, J.W. Porter, Comparative studies of the pigeon liver fatty acid synthetase complex and its subunits. Kinetics of partial reactions and the number of binding sites for acetyl and malonyl groups, J. Biol. Chem. 245 (1970) 4732-4744.
14. W.X. Tian, Q. Liang, B.H. Qu, J. Xiao, The inhibition effect of carboxylates on fatty acid synthetase, Acta Biophys. Sinica 8 (1992) 22-28.
15. P.P. Dodds, M.G.F. Guzman, S.C. Chalberg, G.J. Anderson, S. Kumar, Acetoacetyl-CoA reductase activity of lactating bovine mammary fatty acid synthase, J. Biol. Chem. 256 (1981) 6282-6290.
16. K.A. Dill, D. Shortle, Denatured states of proteins, Annu. Rev. Biochem. 60 (1991) 795-825.
17. E. Haas, C.A. McWherter, H.A. Scheraga, Conformational unfolding in the N-terminal region of ribonuclease A detected by nonradiative energy transfer: Distribution of interresidue distances in the native, denatured and reduced-denatured states, Biopolymers 27 (1998) 1-21.
18. S.J. Wakil, Fatty acid synthase, a proficient multifunctional enzyme, Biochemistry 28 (1989) 4523-4530.
19. K.P. Holzer, W. Liu, G.G. Hammes, Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA, Proc. Natl. Acad. Sci. USA 86 (1989) 4387-4391.
20. H.M. Zhou, X.H. Zhang, Y. Yin, C.L. Tsou, Conformational changes at the active site of creatine kinase at low concentrations of guanidium chloride, Biochem. J. 291 (1993) 103-107.