Plant mitochondrial electrical potential monitored by fluorescence quenching of rhodamine 123

Biologia Plantarum

Volumn 41, Issue 2, 1998, Pages 193-201

  Braidot, E ^a   Petrussa, E ^a   Macri F ^a   Vianello, A ^a  

^a UNIVERSITY OF UDINE   (Italy)

Author keywords

Membrane potential; Mitochondria; Pisum sativum L.

Indexed keywords

EID: 0032436843     PISSN: 00063134     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1001898027218     Document Type: Article

References (17)

1. Bradford, M.M.: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976.
2. 1-ATPase by cationic dyes and amphypathic peptides. - Biochim. biophys. Acta 975: 377-383, 1989.
3. Douce, R.: Mitochondria in Higher Plants: Structure, Function and Biogenesis. - Academic Press, Orlando - San Diego - New York - London - Toronto - Montreal - Sydney - Tokyo 1985.
4. Emaus, R.K., Grunwald, R., Lemasters, J.J.: Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. - Biochim. biophys. Acta 850: 436-448, 1986.
5. Liu, Z., Bushnell, W.R., Brambl, R.: Potentiometric cyanine dyes are sensitive probes for mitochondria in intact plant cells. - Plant Physiol. 84: 1385-1390, 1987.
6. Mai, M.S., Allison, W.S.: Inhibition of oligomycin-sensitive ATPase by cationic dyes, some of which are atypical uncouplers of intact mitochondria. - Arch, Biochem. Biophys. 221: 467-476, 1983.
7. Matzke, M.A., Matzke, A.J.M.: Visualization of mitochondria and nuclei in living plant cells by the use of a potential-sensitive fluorescent dye. - Plant Cell Environ. 9: 73-77, 1986.
8. Mitchell, P.: Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. - Biol. Rev. Cambridge Phil. Soc. 41: 445-502, 1966.
9. Modica-Napolitano, J.S., Weiss, M.J., Chen, L.B., Aprille, J.R.: Rhodamine 123 inhibits bioenergetic function in isolated rat liver mitochondria. - Biochem. biophys. Res. Commun. 118: 717-723, 1984.
10. Moore, A.L., Bonner, W.D., Jr.: Measurements of membrane potentials in plant mitochondria with safranine method. - Plant Physiol. 70: 1271-1276, 1982.
11. Petit, P.X.: Flow cytometric analysis of rhodamine 123 fluorescence during modulation of the membrane potential in plant mitochondria. - Plant Physiol. 98: 279-286, 1992.
12. Petrussa, E., Braidot, E., Nagy, G., Vianello, A., Macrì, F.: Electrical potential dissipation induced by free fatty acids in pea stem mitochondria. - FEBS Lett. 307: 267-271, 1992.
13. Reich, T.J., Iyer, V.N., Haffner, M., La Holbrook, B.L.: The use of fluorescent dyes in the microinjection of alfalfa protoplasts. - Can. J. Bot. 64: 1259-1267, 1986.
14. Smith, J. C.: Potential-sensitive molecular probes in membranes of bioenergetic relevance. - Biochim. biophys. Acta 1016: 1-28, 1990.
15. Vianello, A., Petrussa, E., Macri, F.: ATP/ADP antiporter is involved in uncoupling of plant mitochondria induced by low concentrations of palmitate. - FEBS Lett. 347: 239-242, 1994.
16. Waggoner, A.: Optical probes of membrane potentials. - J. Membr. Biol. 21: 317-334, 1976.
17. Wu, F.-S.; Localization of mitochondria in plant cells by vital staining with rhodamine 123. - Planta 171: 346-357, 1987.