Improvement in the organic solvent stability of pseudomonas lipase by random mutation

Annals of the New York Academy of Sciences

Volumn 864, Issue , 1998, Pages 431-434

  Nakano, Hideo ^a   Ide, Yoshiro ^a   Tsuda, Toshihiko ^a   Yang, Junhao ^a   Ishii, Shouji ^a   Yamane, Tsuneo ^a  

^a Lab of Molecular Biotechnology   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; MUTANT PROTEIN; ORGANIC SOLVENT; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

AMINO ACID SUBSTITUTION; CELL TRANSFORMATION; CONFERENCE PAPER; CONTROLLED STUDY; DNA SEQUENCE; ENZYME RELEASE; ENZYME STABILITY; NONHUMAN; PSEUDOMONAS;

BACTERIA (MICROORGANISMS); PSEUDOMONAS;

EID: 0032432424     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1998.tb10353.x     Document Type: Conference Paper

References (5)

1. 1. IZUMI, T., K. NAKAMURA & T. FUKASE. 1990. Purification and characterization of a thermostable lipase from newly isolated Pseudomonas sp. KWI-56. Agric. Biol. Chem. 54: 1253-1258.
2. 2. IZUMI, T., K. NAKAMURA, Y. SHIMADA, A. SUGIHARA, Y. TOMINAGA & T. FUKASE. 1991. Cloning, nucleotide sequencing, and expression in Escherichia coli of a lipase and its activator genes from Pseudomonas sp. KWI-56. Agric. Biol. Chem. 55: 2357-2379.
3. 3. ROSU, R., Y. UOZAKI, Y. IWASAKI & T. YAMANE. 1997. Repeated use of immobilized lipase for monoacylglycerol production by solid-phase glycerolysis of olive oil. J. Am. Oil Chem. Soc. 74: 445-450.
4. 4. CHEN, K. & F. H. ARNOLD. 1991. Enzyme engineering for nonaqueous solvents: random mutagenesis to enhance activity of subtilisin E in polar organic media. Bio/Technology 9: 1073-1077.
5. 5. UEDA, S., T. YABUTANI, A. MAEHARA & T. YAMANE. 1996. Molecular analysis of the poly(3-hydroxyalkanoate) synthase gene from a methylotrophic bacterium, Paracoccus denitrificans. J. Bacteriol. 178: 774-779.