Fluorescent phalloidin enables visualization of actin without effects on myosin's actin filament sliding velocity and hydrolytic properties in vitro

Journal of Molecular and Cellular Cardiology

Volumn 30, Issue 12, 1998, Pages 2777-2783

  VanBuren, Peter ^a   Begin, Kelly ^a   Warshaw, David M ^a  

^a UNIVERSITY OF VERMONT   (United States)

Author keywords

Actin; ATPase; In vitro motility; Myosin; Phalloidin

Indexed keywords

ACTIN; ADENOSINE TRIPHOSPHATASE; MYOSIN; MYOSIN ADENOSINE TRIPHOSPHATASE; PHALLOIDIN; RHODAMINE;

ACTIN FILAMENT; ANIMAL TISSUE; ARTICLE; CONFORMATIONAL TRANSITION; CRYSTALLOGRAPHY; ENZYME ACTIVITY; ENZYME KINETICS; FLUORESCENCE ANALYSIS; MUSCLE CONTRACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; SKELETAL MUSCLE; VELOCITY; X RAY DIFFRACTION;

ANIMALIA;

EID: 0032426470     PISSN: 00222828     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmcc.1998.0856     Document Type: Article

References (29)

1. BOELS PJ, PFITZER G, 1992. Relaxant effect of phalloidin on triton-skinned microvascular and other smooth muscle preparations. J Muscle Res Cell Motil 13: 71-80.
2. BUKATINA AE, FUCHS F, 1994. Effect of phalloidin on the ATPase activity of striated muscle myofibrils. J Muscle Res Cell Motil 15: 29-36.
3. BUKATINA AE, FUCHS F, BRANDT PW, 1995. Thin filament activation by phalloidin in skinned cardiac muscle. J Mol Cell Cardiol 27: 1311-1315.
4. 2+ responsiveness of skinned cardiac muscle. J Muscle Res Cell Motil 19: 515-523.
5. BURLACU S, JANMEY PA, BOREJDO J, 1992. Distribution of actin filament lengths measured by fluorescence microscopy. Am J Physiol 262: C569-577.
6. CUDA G, PATE E, COOKE R, SELLERS JR, 1997. In vitro actin filament sliding velocities produced by mixtures of different types of myosins. Biophys J 72: 1767-1779.
7. DANCKER P, LOW I, HASSELBACH W, WIELAND T, 1975. Interaction of actin with phalloidin: polymerization and stabilization of F-actin. Biochimica et Biophysica Acta 400: 407-414.
8. DE LA CRUZ EM, POLLARD TD, 1994. Transient kinetic analysis of rhodamine phalloidin binding to actin filaments. Biochemistry 33: 14387-14392.
9. ESTES JE, SELDEN LA, GERSHMAN LC, 1981. Mechanism of action of phalloidin on the polymerization of muscle actin. Biochemistry 20: 708-712.
10. GORDON AM, LAMADRID MA, CHEN Y, LUO Z, CHASE PB, 1997. Calcium regulation of skeletal muscle thin filament motility in vitro. Biophys J 72: 1295-1307.
11. HARRIS DE, WORK SS, WRIGHT RK, ALPERT NR, WARSHAW DM, 1994. Smooth, cardiac and skeletal muscle force and motion generation assessed by cross-bridge mechanical interactions in vitro. J Muscle Res Cell Motil 15: 11-19.
12. HOMSHER E, BIBIANA K, BOBKOVA A, TOBACMAN LS, 1996. Calcium regulation of thin filament movement in an in vitro motility assay. Biophys J 70: 1881-1892.
13. KINOSIAN HJ, SELDEN LA, ESTES JE, GERSHMAN LC, 1993. Actin filament annealing in the presence of ATP and phalloidin. Biochemistry 32: 12353-12357.
14. KRON SJ, SPUDICH JA, 1096. Fluorescent actin filaments move on myosin fixed to a glass surface. Proc Natl Acad Sci USA 83: 6272-6276.
15. LEHMAN W, VIBERT P, CRAIG R, BARANY M, 1996. Actin and the structure of the smooth muscle thin filaments. In: Barany M (Ed) Biochemistry of Smooth Muscle Contraction. San Diego, Calif: Academic Press, pp 47-60.
16. LORENZ M, POPP D, HOLMES KC, 1993. Refinement of the F-actin model against x-ray fiber diffraction data by the use of a directed mutation algorithm. J Mol Biol 234: 826-836.
17. LOW I, DANCKER P, WIELAND T, 1975. Stabilization of F-actin by phalloidin reversal of the destabilizing effect of cytochalasin B. FEBS Lett 54: 263-265.
18. LOWEY S, WALLER GS, TRYBUS KM, 1993. Function of skeletal muscle myosin heavy and light chain isoforms by an in vitro motility assay. J Biol Chem 268: 20414-20418.
19. MARGOSSIAN SS, LOWEY S, 1982. Preparation of myosin and its subfragments from rabbit skeletal muscle. In: Frederiksen DW, Cunningham LW (Eds) Methods Enzymology: Structural and Contractile Proteins. New York: Academic Press, 85: 55-71.
20. MILLIGAN RA, 1996. Protein-protein interactions in the rigor actomyosin complex. Proc Natl Acad Sci USA 93: 21-26.
21. PARDEE JD, SPUDICH JA, 1982. Purification of muscle actin. In: Frederiksen DW, Cunningham LW (Eds) Methods Enzymology: Structural and Contractile Proteins. New York: Academic Press, 85: 164-181.
22. PROCHNIEWICZ-NAKAYAM E, YANAGIDA T, OOSAWA F, 1983. Studies on the conformation of F-actin in muscle fibers in the relaxed state, rigor, and during contraction using fluorescent phalloidin. J Cell Biol 97: 1663-1667.
23. WALLER GS, OUYANG G, SWAFFORD J, VIBERT P, LOWEY S, 1995. A minimal domain from chicken skeletal muscle myosin. J Biol Chem 270: 15348-15352.
24. WARSHAW DM, DESROSIERS JM, WORK SS, TRYBUS KM, 1990. Smooth muscle myosin cross-bridge interactions modulate actin filamentsliding velocity in vitro. J Cell Biol 111: 453-463.
25. WHITE HD, 1982. Special instrumentation and techniques for kinetic studies of contractile systems. In: Frederiksen DW, Cunningham LW (Eds) Methods Enzymology: Structural and Contractile Proteins. New York: Academic Press, 85: 698-708.
26. WIELAND T, DEVRIES JX, SCHAFER A, FAULSTICH H, 1975. Spectroscopic evidence for the interaction of phalloidin with actin. FEBS Lett 54: 73-75.
27. WORK SS, WARSHAW DM, 1992. Computer-assisted tracking of actin filament motility. Anal Biochem 202: 2 75-285.
28. WULF, E, DEBOBEN FA, BAUTZ FA, FAULSTICH H. WIELAND T, 1979. Fluorescent phallotoxin, a tool for the visualization of cellular actin. Proc Natl Acad Sci USA 76: 4498-4502.
29. YANAGIDA T, NAKASE M, NISHIYAMA K, OOSAWA F, 1984. Direct observation of motion of single F-actin filaments in the presence of myosin. Nature 307: 58-60.