A new isolation procedure and further characterisation of the lectin from the red marine alga Ptilota serrata

Journal of Applied Phycology

Volumn 10, Issue 6, 1998, Pages 539-546

  Sampaio, Alexandre H ^a   Rogers, David J ^b   Barwell, Clive J ^b   Saker Sampaio, Silvana ^a   Costa, Francisco H F ^a   Ramos, Márcio V ^a  

^a FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

^b UNIVERSITY OF PORTSMOUTH   (United Kingdom)

Author keywords

Affinity chromatography; Agglutinin; Guar gum; Lectin; Ptilota serrata; Red marine alga; Sugar inhibition

Indexed keywords

3 NITROPHENYL N ACETYL ALPHA DEXTRO GALACTOSIDE; 4 NITROPHENYL N ACETYL BETA DEXTRO GALACTOSIDE; AFFINITY CHROMATOGRAPHY; ASIALOFETUIN; GALACTOSE; GEL FILTRATION; LACTOSE; LECTIN; PROTEIN ISOLATION; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS;

PTILOTA SERRATA;

EID: 0032423859     PISSN: 09218971     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008061327247     Document Type: Article

References (22)

1. Ainouz IL, Sampaio AH, Freitas ALP, Benevides NM, Mapurunga S (1995) Comparative study on hemagglutinins from the red algae Bryothamnion seaforthii and Bryothamnion triquetrum. R. Bras. Fisiol. Veg. 7: 15-19.
2. Appukuttan PS, Surolia A, Bachhawat BK (1977) Isolation of two galactose-binding proteins from Ricinus communis by affinity chromatography. Indian J. Biochem. Biophys. 14: 382-384.
3. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
4. Cavada BS, Moreira RA, Oliveira JTA, Grangeiro TB (1993) Primary structures and functions of plant lectins. R. Bras. Fisiol. Veg. 5: 193-202.
5. Fish BC (1989) Isolation and characterisation of lectins from marine algae, with particular reference of Plumaria elegans and Ptilota serrata. PhD Thesis. University of Portsmouth: 240pp+XIV.
6. Kawagishi H, Wasa T, Murata T, Usui T, Kimura A, Chiba S (1996) Two N-acetyl-D-galactosamine-specific lectins from Phaeolepiota aurea. Phytochemistry 41: 1013-1016.
7. Lonngren J, Goldstein IJ, Bywater R (1976) Cross-linked guaran: a versatile immunosorbent for D-galactopyranosyl binding lectins. FEBS Letters 68: 31-34.
8. Lotan R, Skutelsky E, Danon D, Sharon N (1975) The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea). J. biol. Chem. 250: 5518-5523.
9. Moraes SMD, Cavada BS, Moreira, RA, Roque-Barreira MC, Santos- de-Oliveira R, Pinto VPT, Oliveira JTA (1996) Purification, physicochemical characterization and biological properties of a lectin from Erythrina velutina forma aurantiaca seeds. Braz. J. med. biol. Res. 29: 977-985.
10. Mo H, Goldstein IJ (1994) Isolation and characterization of a Forssman antigen-binding lectin from velvet bean (Mucuna derrigiana) seeds. Glycoconjugate J. 11: 424-431.
11. Rini JM (1995) Lectin structure. Annu. Rev. Biophys. Biomol. Struct. 24: 551-577.
12. Rogers DJ, Blunden G, Topliss JA, Guiry MD (1980) A survey of some marine organisms for haemagglutinins. Bot. mar. 23: 569-577.
13. Rogers DJ, Fish B, Barwell CJ (1990) Isolation and properties of lectins from two red marine algae: Plumaria elegans and Ptilota serrata. In: Kocourek J, Freed DLJ (eds), Lectins: Biology, Biochemistry, Clinical Biochemistry. Sigma Chemical Company, St. Louis, Mo. USA. Vol. 7: 49-52.
14. Rogers DJ, Swain L, Carpenter BG, Critchley AT (1994) Binding of N-acetyl-α-D-galactosamine by lectins from species of the green manine alga genus, Codium. In: Van Driessche E, Fisher J, Beeckmans S, Bog-Hansen TC (eds), Lectins: Biology, Biochemistry, Clinical Biochemistry. Textop, Hellerup (Denmark) 10: 162-165.
15. Sampaio AH (1997) Lectins from Ulva and Ptilota species. PhD Thesis. University of Portsmouth: 191 pp+XV.
16. Sampaio AH, Rogers DJ, Barwell CJ (1996) Characterisation of a new lectin from the green marine alga Ulva laetevirens. In: Van Driessche E, Rouge P, Beeckmans S, Bog-Hansen TC (eds), Lectins: Biology, Biochemistry Clinical Biochemistry. Textop, Hellerup (Denmark) 11: 96-100.
17. Sampaio AH, Rogers DJ, Barwell CJ (1998a) A galactose-specific lectin from the red marine alga Ptilota filicina. Phytochemistry 48: 765-769.
18. Sampaio AH, Rogers DJ, Barwell CJ (1998b) Isolation and characterisation of the lectin from the green marine alga Ulva lactuaca L. Bot. mar. 41: 427-433.
19. Sampietro AR, Vattuone MA (1994) Purification and characterization of a Sarothamnus welwitschii seed lectin. Phytochemistry 35: 841-845.
20. Slomiany DL, Meyer K (1972) Isolation and structural studies of sulphated glycoproteins of hog gastric mucin. J. biol. Chem. 247: 5062-5070.
21. Tyagi T, Gupta MN (1993) Agglutination assay of lectins in presence of specific sugars by using borate. Biotechnology Techniques 7: 885-888.
22. Wilson IA, Skehel JJ, Wiley DC (1981) Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3A resolution. Nature 289: 366-373.