Improved detection and vital staining of Cryptosporidium and Giardia with flow cytometry

Water Science and Technology

Volumn 38, Issue 12, 1998, Pages 61-65

  Medema, G J ^a   Schets, F M ^a   Ketelaars, H ^a   Boschman, G ^a  

^a KIWA Ltd   (Netherlands)

Author keywords

Cryptosporidium; Flow cytometry; Giardia; Water quality

Indexed keywords

MORPHOLOGY; PROTOZOA; PURIFICATION; WATER ANALYSIS; WATER QUALITY;

FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING (FACS);

WATER POLLUTION;

CONFERENCE PAPER; CRYPTOSPORIDIUM; FLOW CYTOMETRY; FLUORESCENCE; GIARDIA; IMMUNOFLUORESCENCE MICROSCOPY; OOCYST; RESERVOIR; WATER CONTAMINATION; WATER QUALITY; WATER SAMPLING;

CRYPTOSPORIDIUM; GIARDIA; PROTOZOA;

EID: 0032423299     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0273-1223(98)00801-4     Document Type: Conference Paper

References (6)

1. A.T. Campbell L.J. Robertson H.V. Smith Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes Appl. Environ. Microbiol. 58 1992 3488 3493
2. J.F. Clancey W.D. Gollnitz Z. Tabib Commercial labs: how accurate are they? J. Am. Wat. Wks. Ass. 86 1994 89 97
3. M.W. LeChevallier W.D. Norton R.G. Lee Occurrence of Giardia and Cryptosporidium spp. in surface water supplies Appl. Environ. Microbiol. 57 1991 2610 2616
4. D.G. Schupp L.S. Erlandsen A new method to determine Giardia cysts viability: correlation of fluorescein diacetate and propidium iodide staining with animal infectivity Appl Environ. Microbiol. 53 1987 704 707
5. A.L. Smith H.V. Smith A comparison of fluorescein diacetate and propidium iodide staining and in vitro excystation for determining Giardia intestinalis cyst viability Parasitology 99 1989 704 709
6. G. Vesey P. Hutton A. Champion N. Ashbolt K.L. Williams A. Warton D. Veal Application of flow cytometric methods for the routine detection of Cryptosporidium and Giardia in water Cytometry 16 1994 1 6