Mapping and cloning of FAD2 gene to develop allele-specific PCR for oleic acid in spring turnip rape (Brassica rapa ssp. oleifera)

Molecular Breeding

Volumn 4, Issue 6, 1998, Pages 543-550

  Tanhuanpää, Pirjo ^a   Vilkki, Juha ^b   Vihinen, Mauno ^c,d  

^a PLANT PRODUCTION RESEARCH   (Finland)

^b Boreal Plant Breeding Ltd   (Finland)

^c UNIVERSITY OF TAMPERE   (Finland)

^d UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Allele specific PCR; Brassica rapa; Fad2; Marker assisted selection; Oleic acid

Indexed keywords

ALLELE; AMINO ACID SUBSTITUTION; CLONING; GENE EXPRESSION; GENE MAPPING; GENETIC CROSS; GENETIC DIFFERENCE; LINKAGE GROUP; NUCLEOTIDE SEQUENCE; OLEIC ACID; PLANT GENETICS; PLANT GROWTH; PLANT PRODUCT; POLYMERASE CHAIN REACTION; QUANTITATIVE TRAIT LOCUS;

AMINO ACIDS; ENZYME ACTIVITY; OLEIC ACID; PLANTS (BOTANY); POLYMERASE CHAIN REACTION;

ACID CONTENT; ALLELE-SPECIFIC PCR; ARABIDOPSIS; BRASSICA; BRASSICA RAPA; DESATURASE; FAD2; LINKAGE GROUPS; MARKER-ASSISTED SELECTION; OLEIFERA;

GENES;

BRASSICA RAPA;

EID: 0032418558     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1009642317634     Document Type: Article

References (28)

1. Appelqvist L-Å: Lipids in Cruciferae. III. Fatty acid composition of diploid and tetraploid seeds of Brassica campestris and Sinapis alba grown under two dimatic extremes. Physiol Plant 21: 615-625 (1968).
2. Appelqvist L-Å: Lipids in Cruciferae IX. The effect of growth temperature and stage of development on the fatty acid composition of leaves siliques and seeds of zero-erucic acid breeding lines of Brassica napus. Physiol Plant 25: 493-502 (1971).
3. Dellaporta SL, Wood J, Hicks JB: A plant DNA minipreparation: version II. Plant Mol Biol Rep 1: 19-21 (1983).
4. Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387-395 (1984).
5. Edwards K, Johnstone C, Thompson C: A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl Acids Res 19: 1349 (1991).
6. Ehlen T, Dubeau L: Detection of ras point mutations by polymerase chain reaction using mutation-specific, inosine-containing oligonucleotide primers. Biochem Biophys Res Commun 160: 441-447 (1989).
7. van Hal JG, Baarsel HM: Influence of temperature and day-light on the fatty acid composition of summer rapeseed (Brassica napus). Proceedings 4th International Rapeseed Conference, (Giessen, Germany 1974), pp. 243-248 (1975).
8. Harwood JL: Recent environmental concerns and lipid metabolism. In: Kader J-C, Mazliak P (eds) Plant Lipid Metabolism, pp. 361-368. Kluwer Academic Publishers, Dordrecht, Netherlands (1995).
9. Huang M-M, Arnheim N, Goodman MF: Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR. Nucl Acids Res 20: 4567-4573 (1992).
10. Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E, Nakamura Y, Miyajima N, Hirosawa M, Sugiura M, Sasamoto S, Kimura T, Hosouchi T, Matsuno A, Muraki A, Nakazaki N, Naruo K, Okumura S, Shimpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S: Sequence analysis of the genome of the unicellular cyanobacteriurn Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res 3: 109-136 (1996).
11. Kirsch C, Takamiya-Wik M, Reinold S, Hahlbrock K, Somssich IE: Rapid, transient, and highly localized induction of plastidial ω-3 fatty acid desaturase mRNA at fungal infection sites in Petroselinum crispum. Proc Natl Acad Sci USA 94: 2079-2084 (1997).
12. Kodama H, Akagi H, Kusumi K, Fujimura T, Iba K: Structure, chromosomal location and expression of a rice gene encoding the microsome ω-3 fatty acid desaturase. Plant Mol Biol 33: 493-502 (1997).
13. Kwok S, Kellogg DE, McKinney N, Spasic D, Goda L, Levenson C, Sninsky JJ: Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucl Acids Res 18: 999-1005 (1990).
14. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L: MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174-181 (1987).
15. Lindqvist Y, Huang W, Schneider G, Shanklin J: Crystal structure of Δ-9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other di-iron proteins EMBO J 15: 4081-4092 (1996).
16. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
17. Newton CR, Graham A, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, Smith JC, Markham AF: Analysis of any point mutation in DNA. The amplification refractory mutation system. Nucl Acids Res 17:2503-2515 (1989).
18. Nichols WC, Liepnieks JJ, McKusick VA, Benson MD: Direct sequencing of the gene for Maryland/German familial amyloidotic polyneuropathy type II and genotyping by allele-specific enzymatic amplification. Genomics 5: 535-540 (1989).
19. Okayama H, Curiel DT, Brantly ML, Holmes MD, Crystal RG: Rapid, nonradioactive detection of mutations in the human genome by allele-specific amplification. J Lab Clin Med 114: 105-113 (1989).
20. Okuley J, Lightner J, Feldmann K, Yadav N, Lark E: Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell 6: 147-158 (1994).
21. Rost B: PHD:Predicting one-dimensional protein structure by profile-based neural networks. Meth Enzymol 266: 525-539 (1996).
22. Tanhuanpää PK, Vilkki HJ, Vilkki JP, Pulli SK: Genetic polymorphism at RAPD loci in spring turnip rape (Brassica rapa ssp. oleifera). Agric Sci Finl 2: 303-310 (1993).
23. Tanhuanpää PK, Vilkki JP, Vilkki HJ: Mapping of a QTL for oleic acid concentration in spring turnip rape (Brassica rapa ssp. oleifera). Theor Appl Genet 92: 952-956 (1996).
24. Tanhuanpää PK, Vilkki JP, Vilkki HJ: A linkage map of spring turnip rape based on RFLP and RAPD markers. Agric Food Sci Finl 5: 209-217 (1996).
25. Teutonico RA, Osborn TC: Mapping of RFLP and qualitative trait loci in Brassica rapa and comparison to the linkage maps of B. napus, B. oleracea, and Arabidopsis thaliana. Theor Appl Genet 89: 885-894 (1994).
26. Thies W: Die Biogenese von Linol- und Linolensäure in den Samen höherer Pflanzen, insbesondere Raps und Rübsen, als Problem der Ölpflanzenzüchtung. Angew Bot 42: 140-154 (1968).
27. Thoma S, Kaneko Y, Somerville C: A non-specific lipid transfer protein from Arabidopsis is a cell wall protein. Plant J 3: 427-436 (1993).
28. Wu DY, Ugozzoli L, Pal BK, Wallace RB: Allele-specific enzymatic amplification of β-globin genomic DNA for diagnosis of sickle cell anemia. Proc Natl Acad Sci USA 86: 2757-2760 (1989).