Ethyl isopropylamiloride downregulates Na,K-ATPase gene expression which confers cytotoxicity in primary proximal tubule cell cultures

Chinese Journal of Physiology

Volumn 41, Issue 4, 1998, Pages 195-202

  Chiu, Hui Min ^a   Lin, Hsi Hui ^a   Tang, Ming Jer ^a  

^a NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

Amiloride; Cell toxicity; Intracellular pH; Na,K ATPase; Primary culture; Proximal tubule

Indexed keywords

5 (N ETHYL N ISOPROPYL)AMILORIDE; ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); AMILORIDE DERIVATIVE; LACTATE DEHYDROGENASE;

ANIMAL CELL; ARTICLE; BASOLATERAL MEMBRANE; CELL PH; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DRUG MECHANISM; ENZYME ACTIVITY; ION EXCHANGE; KIDNEY TUBULE CELL; METABOLIC ACIDOSIS; NONHUMAN; PROTEIN EXPRESSION; RABBIT;

EID: 0032418309     PISSN: 03044920     EISSN: 26660059     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Aronson, P. S., and W. Boron. Na/H exchange, intracellular pH and cell function. Academic Press, New York. 1986.
2. Biemesderfer, D., J. Pizzonia, A. Abu-alfa, M. Exner, R. Reilly, P. Igarashi, and P.S. Aronson. NHE3: A Na/H exchanger isoform of renal brush border. Am, J. Physiol. 265 (Renal Fluid Electrolyte Physiol. 34): F736-F742, 1993.
3. Brant, S. R., C. H. C. Yun, M. Donowitz, and C. M. Tse. Cloning, tissue distribution, and functional analysis of the human Na/H exchanger isoform NHE3. Am J. Physiol. 269 (Cell Physiol. 38): C198-C206, 1995.
4. Counillon, L., W. Scholz, H. J. Lang, and J. Pouyssegur. Pharmacological characterization of stably transfected Na/H antiporter isoforms using amiloride analogs and a new inhibitor exhibiting anti-ischemic properties. Mol. Pharmacology 44: 1041-1045, 1993.
5. + exchanger. Am. J. Physiol. 255: F552-F557, 1988.
6. Grinstein, S., and A. Rothstein. Mechanisms of regulation of the Na/H exchanger. J. Membr. Biol. 90: 1-12, 1986.
7. Grinstein, S., S. D. Rotin, and M. J. Mason. Na/H exchange and growth factor-induced cytosolic pH change. Role in cellular proliferation. Biochim. Biophys. Acta 988: 73-97, 1989.
8. Haggerty, J. G., N. Agarwal, R. F. Reilly, E. A. Adelberg, and C. W. Slayman. Pharmacologically different Na/H antipoters on the apical and basolateral surfaces of cultured porcine kidney cells (LLC-PK1). Proc. Natl. Acad. Sci. U.S.A. 85: 6797-6801, 1988.
9. Harris, R. C., J. L. Seifter, and C. Lechene. Coupling of Na-H exchange and Na-K pump activity in cultured rat proximal tubule cells. Am. J. Physiol. 251 (Cell Physiol. 20): C815-C824, 1986.
10. Hildebrandt, F., J. H. Pizzonia, R. F. Reilly, N. A. Reboucas, C. Sardet, J. Pouyssegur, C. W. Slayman, P.S. Aronson, and P. Igarashi. Cloning, sequence and tissue distribution of a rabbit renal Na/H exchanger transcript. Biochim. Biophys. Acta 1129: 105-108, 1991.
11. Hoffman, E. K., and L. O. Simonsen. Membrane mechansims in volume and pH regulation in vertebrate cells. Physiol. Rev. 69: 315-382, 1989.
12. Lee, Y. J., H. H. Lin, and M. J. Tang. Glucocorticoid upregulates Na,K-ATPAse α- and β-mRNA via an indirect mechanism in proximal tubule cell primary culture. Am. J. Physiol. 268 (Renal Fluid Electrolyte Physiol. 37): F862-F867, 1995.
13. Kleyman T. R. and E. J. Cragoe, Jr. Amiloride and its analogs as tools in the study of ion transport. J. Mem. Biol. 1105: 1-21, 1988.
14. Lo, C. S., and I. S. Edelman. Effect of triiodothyronine on the synthesis and degradation of renal cortical (Na,K) adenosine triphosphatase. J. Biol. Chem. 251: 7834-7840, 1976.
15. Lowry, O. H., N. J. Rosebrough, A.L. Fair, and R.J. Randall. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275, 1951.
16. Maidorn, R. P., E. J. Cragoe, Jr and I.F. Tannock. Therapeutic potential of analogues of amiloride: Inhibition of the regulation of intracellular pH as a possible mechanism of tumour selective. Br. J. Cancer. 67: 297-303, 1993.
17. Musgrove, E., C Rugg, and D. Hedley. Flow cytometric measurement of cytoplasmic pH: a critical evaluation of available fluorochromes. Cytometry 7: 347-355, 1986.
18. + entry. Am. J. Physiol. 270 (Cell Physiol. 39): C600-C607, 1996.
19. Rossier, B. C., K. Geering, and J. P. Kraehenbuhl. Regulation the sodium pump: How and why? TIBS. 12: 483-487, 1987.
20. Sweadner, K. J. Isozyme of Na,K-ATPase. Biochim. Biophys. Acta. 988: 185-220, 1989.
21. Tang, M-J., Y.-R. Cheng, and H.-H. Lin. Role of apoptosis in growth and differentiation of proximal tubule cells in primary cultures. Biochem. Biophys. Res. Commun. 218: 658-664, 1996.
22. + increases Na,K-ATPase α and β mRNA and protein abundance in cultured renal proximal tubule cells. Am. J. Physiol. (Cell Physiol. 32): C436-C442, 1992.
23. Tang, M.-J., K. R. Suresh, and R. L. Tannen. Carbohydrate metabolism by primary cultures of rabbit proximal tubules. Am. J. Physiol. 256 (Cell Physiol. 25): C532-C539, 1989.
24. Tang, M.-J., and R. L. Tannen. Metabolic substrates alter attachment and differentiated functions of proximal tubule cell culture. J. Am. Soc. Nephrol. 4: 1908-1911, 1994.
25. Tang, M.-J., H.-H. Lin, and Y.-K. Wang. Expression and regulation of Na,K-ATPase in primary culture of proximal tubule cells. Chin. J. Physiol. 37: 21-29, 1994.
26. Tang, M.-J., Y.-K. Wang, and H.-H. Lin. Butyrate and TGF-β dowregulate Na,K-ATPase expression in cultured proximal tubule cells. Biochem. Biophysic. Res. Commun. 215: 57-66, 1995.
27. Tse, C.-M., S. R. Brant, M.S. Walker, J. Pouyssegur, and M. Donowitz. Cloning and sequencing of a rabbit cDNA encoding an intestinal and kidney-specific Na/H exchanger isoform (NHE-3). J. Biol. Chem., 267: pp. 9340-9346, 1992.
28. Tse, C.-M., S. A. Levine, C. H. C. Yun, M. H. Montrose, P. J. Little, J. Pouyssegur, and M. Donowitz. Cloning and expression of a Rabbit cDNA encoding a serum-activated ethyliso-propylamiloride-resistant epithelial Na/H exchanger isoform (NHE2). J. Biol. Chem. 268: 11917-11924, 1993.
29. Tse, C.-M., S. Levine, C. H. Yun, S. R. Brant, J. Pouyssegur, M. H. Montrose, and M. Donowitz. Molecular properties, kinetics and regulation of mammalian Na/H Exchanger. Cell Physiol. Biochem. 4: 282-300, 1994.
30. Yamamoto, K., U. Ikeda, S. Yoshitane, Y. Tsuruya, A. Oguchi, K. Okada, S. Ishikawa, T. Saito, K. Kawakami, Y. Hara, and K. Shimada. Regulation of Na,K-adenosine triphosphatase gene expression by sodium ions in cultured neontal rat cardiocytes. J. Clin. Invest. 92: 1889-1895, 1993