An improved method to obtain highly differentiated monolayers of human bronchial epithelial cells

In Vitro Cellular and Developmental Biology - Animal

Volumn 34, Issue 6, 1998, Pages 478-481

  Galietta, Luis J V ^a   Lantero, Sabina ^a   Gazzolo, Andrea ^a   Sacco, Oliviero ^a   Romand, Luca ^a   Rossi, Giovanni A ^a   Zegarra Moran, Olga ^a  

^a G GASLINI INSTITUTE   (Italy)

Author keywords

Bronchial epithelium; Cystic fibrosis; Ion transport; Short circuit current; Transepithelial resistance

Indexed keywords

ARTICLE; BRONCHUS MUCOSA; CELL DIFFERENTIATION; CYSTIC FIBROSIS; ELECTROPHYSIOLOGY; GENE TRANSFER; HUMAN; HUMAN CELL; ION TRANSPORT; MONOLAYER CULTURE; RESPIRATORY EPITHELIUM;

BRONCHUS;

EID: 0032418043     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-998-0081-2     Document Type: Article

References (15)

1. Boucher, R. C. Human airway ion transport. Part one. Am. J. Resp. Crit. Care 150:271-281; 1994.
2. Gruenert, D. C.; Basbaum, C. B.; Widdicombe, J. H. Long-term culture of normal and cystic fibrosis epithelial cells grown under serum-free conditions. In Vitro Cell. Dev. Biol. 26:411-418; 1990.
3. Hull, J.; Harris, A. Limitations of cell culture of airway epithelium collected by a nasal brushing technique. In Vitro Cell. Dev. Biol. 30:488-489; 1994.
4. 3- conductance through CFTR in human airway epithelia. Am. J. Physiol. 272:L752-L761; 1997.
5. Knowles, M.; Murray, G.; Shallal, J. et al. Bioelectric properties and ion flowacross excised human bronchi. J. Appl. Physiol. 56:868-877;1984.
6. + in cystic fibrosis: is it important? Trends Pharmacol. Sci. 16:120-121; 1995.
7. Lechner, J. F.; LaVeck, M. A. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Cult. Methods 9:43-18; 1985.
8. Reid, L. M. Defining hormone and matrix requirements for differentiated epithelia. In: Pollard, J. W.; Walker, J. M., ed. Animal cell culture: methods in molecular biology. Vol. 5. Clifton, NJ: Humana Press; 1990:237-276.
9. Sacco, O.; Romberger, D.; Rizzino, A., et al. Spontaneous production of transforming growth factor-β2 by primary cultures of bronchial epithelial cells. J. Clin. Invest. 90:1379-1385; 1992.
10. + channel regulators on cystic fibrosis transmembrane conductance regulator chloride currents. J. Gen. Physiol. 100:573-591; 1992.
11. Stutts, M. J.; Canessa, C. M.; Olsen, J. C., et al. CFTR as a cAMP-dependent regulator of sodium channels. Science 269:847-850; 1995.
12. Widdicombe, J. H. Use of cultured airway epithelial cells in studies of ion transport. Am. J. Physiol. 258:L13-18; 1990.
13. Yamaya, M.; Finkbeiner, W. E.; Chun, S. Y., et al. Differentiated structure and function of cultures from human tracheal epithelium. Am. J. Physiol. 262:L713-724; 1992.
14. Yankaskas, J. R.; Cotton, C. U.; Knowles, M. R., et al. Culture of human nasal epithelial cells on collagen matrix supports. Am. Rev. Respir. Dis. 132:1281-1287; 1985.
15. - currents activated by extracellular nucleotides in human bronchial cells. J. Membr. Biol. 156:297-305; 1997.