Mutational analysis of the Escherichia coli K-12 TolA N-terminal region and characterization of its TolQ-interacting domain by genetic suppression

Journal of Bacteriology

Volumn 180, Issue 24, 1998, Pages 6433-6439

  Germon, Pierre ^a   Clavel, Thierry ^a   Vianney, Anne ^a   Portalier, Raymond ^a   Lazzaroni, Jean Claude ^a  

^a UNIVERSITÉ LYON 1   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL MUTATION; CELL MEMBRANE; ESCHERICHIA COLI; GENE REPRESSION; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI; ESCHERICHIA COLI K12; GOSSWEILERODENDRON BALSAMIFERUM; NEGIBACTERIA;

EID: 0032417321     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.24.6433-6439.1998     Document Type: Article

References (45)

1. Bénédetti, H., C. Lazdunski, and R. Lloubès. 1991. Protein import into Escherichia coli; colicins A and E1 interact with a component of their translocation system. EMBO J. 10:1989-1995.
2. Bénédetti, H., and V. Géli. 1996. Colicin transport, channel formation and inhibition, p. 665-691. In W. N. Konings, H. R. Kaback, and J. S. Lolkema (ed.), Handbook of biological physics, vol. 2. Elsevier Science B.V., Amsterdam, The Netherlands.
3. Bernadac, A., M. Gavioli, J. C. Lazzaroni, S. Raina, and R. Lloubes. 1998. Escherichia coli tol-pal mutants form outer membrane vesicles. J. Bacteriol. 180:4872-4878.
4. Bouveret, E., R. Derouiche, A. Rigal, R. Lloubès, C. Lazdunski, and H. Bénédetti. 1995. Peptidoglycan-associated lipoprotein-TolB interaction. A possible key to explaining the formation of contact sites between the inner and outer membranes of Escherichia coli. J. Biol. Chem. 270:11071-11077.
5. Bouveret, E., A. Rigal, C. Lazdunski, and H. Bénédetti. 1997. The N-terminal domain of colicin E3 interacts with TolB which is involved in the colicin translocation step. Mol. Microbiol. 23:909-920.
6. Bradbeer, C. 1993. The proton motive force drives the outer membrane transport of cobalamine in Escherichia coli. J. Bacteriol. 175:3146-3150.
7. Braun, V. 1989. The structurally related exbB and tolQ genes are interchangeable in conferring TonB-dependent colicin, phage, and albomycin sensitivity. J. Bacteriol. 171:6387-6390.
8. Braun, V., K. Günter, and K. Hantke, 1991. Transport of iron across the outer membrane. Biol. Metals 4:14-22.
9. Braun, V., and C. Herrmann. 1993. Evolutionary relationship of uptake systems for biopolymers in Escherichia coli: cross complementation between the TonB-ExbB-ExbD and the TolA-TolQ-TolR proteins. Mol. Microbiol. 8: 261-268.
10. Cadwell, R. C., and G. F. Joyce. 1995. Mutagenic PCR, p. 583-589. In C. W. Dieffenbach and G. S. Dveksler (ed.), PCR primer, a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
11. Click, E. M., and R. E. Webster. 1997. Filamentous phage infection: required interactions with the TolA protein. J. Bacteriol. 1079:6464-6471.
12. Derouiche, R., H. Bénédetti, J. C. Lazzaroni, C. Lazdunski, and R. Lloubès. 1995. Protein complex within Escherichia coli inner membrane: TolA N-terminal domain interacts with TolQ and TolR proteins. J. Biol. Chem. 270: 11078-11084.
13. Derouiche, R., M. Gavioli, H. Bénédetti, A. Prilipof, C. Lazdunski, and R. Lloubes. 1996. TolA central domain interacts with Escherichia coli porins. EMBO J. 15:6408-6415.
14. Eick-Helmerich, K., and V. Braun. 1989. Import of biopolymers into Escherichia coli: nucleotide sequences of the exbB and exbD genes are homologous to those of the tolQ and tolR genes, respectively. J. Bacteriol. 171:5127-5134.
15. Garinot-Schneider, C., C. N. Penfold, G. R. Moore, C. Kleanthous, and R. James. 1997. Identification of residues in the putative TolA box which are essential for the toxicity of the endonuclease toxin colicin E9. Microbiology 143:2931-2938.
16. Hibert, M. F., S. Trump-Kallmeyer, J. Hofloch, and A. Bruinuels. 1993. This is not a G-protein coupled receptor. Trends Pharmacol. Sci. 14:7-13.
17. Ishidate, K., E. S. Creeger, J. Zrike, S. Deb, B. Glauner, T. J. MacAlister, and L. I. Rothfield. 1986. Isolation of differentiated membrane domains from Escherichia coli and Salmonella typhimurium, including a fraction containing attachment sites between the inner and outer membranes and the murein skeleton of the cell envelope. J. Biol. Chem. 261:428-443.
18. James, R., C. Kleanthous, and G. R. Moore. 1996. The biology of E colicins: paradigms and paradoxes. Microbiology 142:1569-1580.
19. Jaskula, J. C., T. E. Letain, S. K. Roof, J. T. Skare, and K. Postle. 1994. The role of the TonB amino terminus in energy transduction between membranes. J. Bacteriol. 170:2326-2338.
20. Journet, L., and H. Bénédetti. Personal communication.
21. Kampfenkel, K., and V. Braun. 1992. Membrane topology of the Escherichia coli ExbD protein. J. Bacteriol. 174:5485-5487.
22. Kampfenkel, K., and V. Braun. 1992. Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli. J. Biol. Chem. 268:6050-6057.
23. Karlsson, M., K. Hannavy, and C. F. Higgins. 1993. A sequence-specific function for the N-terminal signal-like sequence of the TonB protein. Mol. Microbiol. 8:379-388.
24. Koebnieck, R. 1993. The molecular interaction between components of the TonB-ExbBD-dependent and the TolQRA-dependent bacterial uptake systems. Mol. Microbiol. 9:219.
25. Larsen, R. A., G. E. Wood, and K. Postle. 1993. The conserved proline-rich motif is not essential for energy transduction by Escherichia coli protein. Mol. Microbiol. 10:943-953.
26. Larsen, R. A., M. T. Thomas, and K. Postle. 1994. Partial suppression of an Escherichia coli TonB transmembrane domain mutation (ΔV17) by a missense mutation in ExbB. Mol. Microbiol. 13:627-640.
27. Lazdunski, C. J. 1995. Colicin import and pore formation: a system for studying protein transport across membranes? Mol. Microbiol. 16:1059-1066.
28. Lazzaroni, J. C., and R. C. Portalier. 1992. The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL). Mol. Microbiol. 6:735-742.
29. Lazzaroni, J. C., A. Vianney, J. L. Popot, H. Bénédetti, F. Samatey, C. Lazdunski, R. Portalier, and V. Géli. 1995. Transmembrane α-helix interactions are required for the functional assembly of the Escherichia coli Tol complex. J. Mol. Biol. 246:1-7.
30. Letain, T. E., and K. Postle. 1997. TonB protein appears to transduce energy by shuttling between the cytoplasmic membrane and the outer membrane in Escherichia coli. Mol. Microbiol. 24:271-283.
31. Levengood, S. K., W. F. Beyer, and R. E. Webster. 1991. TolA: a membrane protein involved in colicin uptake contains an extended helical region. Proc. Natl. Acad. Sci. USA 88:5939-5943.
32. Miller, J. H. 1992. A short course of bacterial genetics: a laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
33. Müller, M. M., A. Vianney, J. C. Lazzaroni, R. C. Portalier, and R. E. Webster. 1993. Membrane topology of the Escherichia coli TolR protein required for cell envelope integrity. J. Bacteriol. 175:6059-6061.
34. Postle, K. 1993. TonB and energy transduction between membranes. J. Bioenerg. Biomembr. 25:591-601.
35. Postle, K., and J. T. Skare. 1988. Escherichia coli TonB protein is exported from the cytoplasm without proteolytic cleavage of its N-terminus. J. Biol. Chem. 263:11000-11007.
36. Riechmann, L., and P. Holliger. 1997. The C-terminal domain of TolA is the coreceptor for filamentous phage infection in E. coli. Cell 90:351-360.
37. Rigal, A., E. Bouveret, R. Lloubès, C. Lazdunski, and H. Bénédetti. 1997. The TolB protein interacts with the porins of Escherichia coli. J. Bacteriol. 179:7274-7279.
38. Rutz, J. M., J. Liu, J. A. Lyons, J. Goranson, S. K. Armstrong, M. A. McIntosh, J. B. Feix, and P. E. Klebba. 1992. Formation of a gated channel by a ligand-specific transport in the bacterial outer membrane. Science 258: 471-475.
39. Skare, J. T., and K. Postle. 1991. Evidence for a TonB-dependent energy transduction complex in Escherichia coli. Mol. Microbiol. 5:2883-2890.
40. Traub, I., S. Gaisser, and V. Braun. 1993. Activity domains of the TonB protein. Mol. Microbiol. 8:409-423.
41. Valentin-Hansen, P., B. Albrechtsen, and J. E. Love Larsen. 1986. DNA-protein recognition: demonstration of three genetically separated operator elements that are required for repression on the Escherichia coli deoCABD promoters by the DeoR repressor. EMBO J. 5:2015-2021.
42. Vianney, A., T. M. Lewin, W. E. Bayer, J. C. Lazzaroni, R. Portalier, and R. E. Webster. 1994. Membrane topology and mutational analysis of the TolQ protein of Escherichia coli required for the uptake of macromolecules and cell envelope integrity. J. Bacteriol. 176:822-829.
43. Vianney, A., M. Muller, T. Clavel, J. C. Lazzaroni, R. Portalier, and R. E. Webster. 1996. Characterization of the tol-pal region of Escherichia coli: translational control of tolR expression by TolQ and identification of a new open reading frame downstream of pal encoding a periplasmic protein. J. Bacteriol. 178:4031-4038.
44. Webster, R. E. 1991. The tol gene products and the import of macromolecules into Escherichia coli. Mol. Microbiol. 5:1005-1011.
45. Witkowski, A., J. Nagger, H. E. Witkowska, Z. I. Rhandawa, and S. Smith. 1992. Utilization of an active serine 101-cysteine mutant to demonstrate the proximity of the catalytic serine 101 and histidine 237 residues in thioesterase II. J. Biol. Chem. 267:18488-18492.