Microinjection of antibodies to centromere protein CENP-A arrests cells in interphase but does not prevent mitosis

Chromosoma

Volumn 107, Issue 6-7, 1998, Pages 397-405

  Figueroa, Javier ^a   Saffrich, Rainer ^b   Ansorge, Wilhelm ^b   Valdivia, Manuel ^a  

^a UNIVERSITY OF CÁDIZ   (Spain)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; AUTOANTIGEN; DNA BINDING PROTEIN; HISTONE H3; NOCODAZOLE;

CELL CYCLE; CENTROMERE; CONFERENCE PAPER; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTERPHASE; MICROINJECTION; MICROSCOPY; MITOSIS; MITOSIS INHIBITION;

ANIMALS; ANTIBODY SPECIFICITY; AUTOANTIGENS; CATTLE; CELL CYCLE; CELL LINE; CENTROMERE; CHROMOSOMAL PROTEINS, NON-HISTONE; DEER; FLUORESCENT ANTIBODY TECHNIQUE; HAPLORHINI; HELA CELLS; HUMANS; INTERPHASE; KINETOCHORES; MICROINJECTIONS; MITOSIS; MITOTIC SPINDLE APPARATUS;

EID: 0032417220     PISSN: 00095915     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004120050323     Document Type: Conference Paper

References (38)

1. Bernat RL, Borisy GG, Rothfield NF, Earnshaw WC (1990) Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis. J Cell Biol 111:1519-1533
2. Brinkley BR, Ouspenski I, Zinkowski RP (1992) Structure and molecular organization of the centromere-kinetochore complex. Trends Cell Biol 2:15-21
3. Choo KHA (1997) The centromere. Oxford University Press, Oxford
4. Compton DA, Yen TJ, Cleveland DW (1991) Identification of novel centromere/kinetochore-associated proteins using monoclonal antibodies generated against human mitotic chromosome scaffolds. J Cell Biol 112:1083-1097
5. Cooke CA, Bernat RL, Earnshaw WC (1990) CENP-B: a major human centromere protein located beneath the kinetochore. J Cell Biol 110:1475-1488
6. Earnshaw WC, Rothfield N (1985) Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma. Chromosoma 91:313-321
7. Earnshaw WC, Tomkiel JE (1992) Centromere and kinetochore structure. Curr Opin Cell Biol 4:86-93
8. Guldner HH, Lakomek HJ, Bautz FA (1984) Human anti-centromere sera recognise a 19.5 kD non-histone chromosomal protein from HeLa cells. Clin Exp Immunol 58:13-20
9. He D, Zeng C, Woods K, Zhong L, Turner D, Busch RK, Brinkley BR, Busch H (1998) CENP-G: a new centromeric protein that is associated with the alpha-1 satellite DNA subfamily. Chromosoma 107:189-197
10. Herr S, Bastian T, Pepperkok R, Boulin C, Ansorge W (1993) A fully automated image acquisition and analysis system for low light level fluorescence microscopy. Methods Mol Cell Biol 4:164-170
11. Jabs EW, Persico MG (1987) Characterization of human centromeric regions of specific chromosomes by means of alphoid DNA sequences. Am J Hum Genet 41:374-390
12. Kalitsis P, MacDonald AC, Newson AJ, Hudson DF, Choo KH (1998) Gene structure and sequence analysis of mouse centromere proteins A and C. Genomics 47:108-114
13. Kingwell B, Rattner JB (1987) Mammalian kinetochore/centromere composition: a 50 kDa antigen is present in the mammalian kinetochore/centromere. Chromosoma 95:403-407
14. Liao H, Winkfein RJ, Mack G, Rattner JB, Yen TJ (1995) CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis. J Cell Biol 130:507-518
15. Masumoto H, Masukata H, Muro Y, Nozaki N, Okazaki T (1989) A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromeric satellite. J Cell Biol 109:1963-1973
16. Moroi Y, Peebles C, Fritzler MJ, Steigerwald J, Tan EM (1980) Autoantibody to centromere (kinetochore) in scleroderma sera. Proc Natl Acad Sci USA 77:1627-1631
17. O'Keefe RT, Henderson SC, Spector DL (1992) Dynamic organization of DNA replication in mammalian cell nuclei: spatially and temporally defined replication of chromosome-specific alpha-satellite DNA sequences. J Cell Biol 116:1095-1110
18. Palmer DK, O'Day K, Wener MH, Andrews BS, Margolis RL (1987) A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones. J Cell Biol 104:805-815
19. Pepperkok R, Schneider C, Philipson L, Ansorge W (1988) Single cell assay with an automated capillary microinjection system. Exp Cell Res 178:369-376
20. Rattner JB, Rao A, Fritzler MJ, Valencia DW, Yen TJ (1993) CENP-F is a ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization. Cell Motil Cytoskeleton 26:214-226
21. Saitoh H, Tomkiel J, Cooke CA, Ratrie H 3d, Maurer M, Rothfield NF, Earnshaw WC (1992) CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate. Cell 70:115-125
22. Schaar BT, Chan GK, Maddox P, Salmon ED, Yen TJ (1997) CENP-E function at kinetochores is essential for chromosome alignment. J Cell Biol 139:1373-1382
23. Shelby RD, Vafa O, Sullivan KF (1997) Assembly of CENP-A into centromeric chromatin requires a cooperative array of nucleosomal DNA contact sites. J Cell Biol 136:501-513
24. Stein GS, Stein JL, Lian JB, et al (1994) Synchronization of normal diploid and transformed mammalian cells. In: Celis JE (ed) Cell biology: a laboratory handbook. Academic Press, pp 282-287
25. Sugimoto K, Muro Y, Himeno M (1992) Anti-helix-loop-helix domain antibodies: discovery of autoantibodies that inhibit DNA binding activity of human centromere protein B (CENP-B). J Biochem (Tokyo) 111:478-483
26. Sullivan KF, Hechenberger M, Masri K (1994) Human CENP-A contains a histone H3 related histone fold domain that is required for targeting to the centromere. J Cell Biol 127:581-592
27. Tomkiel JE, Cooke CA, Saitoh H, Bernat RL, Earnshaw WC (1994) CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase. J Cell Biol 125:531-545
28. Valdivia MM, Brinkley BR (1985) Fractionation and initial characterization of the kinetochore from mammalian metaphase chromosomes. J Cell Biol 101:1124-1134
29. Valdivia MM, Figueroa J, Iglesias C, Ortiz M (1998) A novel centromere monospecific serum to a human autoepitope on the histone H3-like protein CENP-A. FEBS Lett 422:5-9
30. Warburton PE, Cooke CA, Bourassa S, Vafa O, Sullivan BA, Stetten G, Gimelli G, Warburton D, Tyler-Smith C, Sullivan KF, et al (1997) Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres. Curr Biol 7:901-904
31. Williams BC, Karr TL, Montgomery JM, Goldberg ML (1992) The Drosophila l(1)zw10 gene product, required for accurate mitotic chromosome segregation, is redistributed at anaphase onset. J Cell Biol 118:759-773
32. Wordeman L, Mitchison TJ (1995) Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis [see comments]. J Cell Biol 128:95-104
33. Wordeman L, Earnshaw WC, Bernat RL (1996) Disruption of CENP antigen function perturbs dynein anchoring to the mitotic kinetochore. Chromosoma 104:551-560
34. Yang CH, Tomkiel J, Saitoh H, Johnson DH, Earnshaw WC (1996) Identification of overlapping DNA-binding and centromere-targeting domains in the human kinetochore protein CENP-C. Mol Cell Biol 16:3576-3586
35. Yen TJ, Compton DA, Wise D, Zinkowski RP, Brinkley BR, Earnshaw WC, Cleveland DW (1991) CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase. EMBO J 10:1245-1254
36. Yen TJ, Li G, Schaar BT, Szilak I, Cleveland DW (1992) CENP-E is a putative kinetochore motor that accumulates just before mitosis. Nature 359:536-539
37. Yoda K, Kitagawa K, Masumoto H, Muro Y, Okazaki T (1992) A human centromere protein, CENP-B, has a DNA binding domain containing four potential alpha helices at the NH2 terminus, which is separable from dimerizing activity. J Cell Biol 119:1413-1427
38. Zieve GW, Turnbull D, Mullins JM, McIntosh JR (1980) Production of large numbers of mitotic mammalian cells by use of the reversible microtubule inhibitor nocodazole. Nocodazole accumulated mitotic cells. Exp Cell Res 126:397-405