Non-radiochemical 3-hydroxy-3-methylglutaryl-coenzyme a synthase assay by reversed-phase HPLC without using ion-pair reagent

Journal of Fermentation and Bioengineering

Volumn 86, Issue 5, 1998, Pages 523-526

  Tachibana, Akira ^a,b   Yano, Yoshihisa ^a   Otani, Shuzo ^a   Taniguchi, Makoto ^b  

^a OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b Osaka Prefecture University   (Japan)

Author keywords

Archaeal isoprenoid biosynthesis; HMG CoA synthase; Non radiochemical assay; Pyrococcus furiosus

Indexed keywords

BIOASSAY; BIOSYNTHESIS; CATALYST ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SUBSTRATES;

DETECTION LIMITS;

COENZYMES;

HYDROXYMETHYLGLUTARYL COENZYME A SYNTHASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SUBSTRATE; NONHUMAN; PYROCOCCUS FURIOSUS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ARCHAEA; PYROCOCCUS FURIOSUS;

EID: 0032417091     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0922-338X(98)80166-9     Document Type: Article

References (21)

1. Zhang, D. and Poulter, C. D.: Biosynthesis of archaebacterial ether lipids. Formation of isoprene ethers by a prenyl transfer reaction. J. Am. Chem. Soc., 115, 1270-1277 (1993).
2. Tachibana, A., Tanaka, T., Taniguchi, M., and Oi, S.: Purification and characterization of geranylgeranyl diphosphate synthase from Methanobacterium thermoformicicum SF-4. Biosci. Biotech. Biochem., 57, 1129-1133 (1993).
3. Tachibana, A., Tanaka, T., Taniguchi, M., and Oi, S.: Potassium-stimulating mechanism of geranylgeranyl diphosphate synthase of Methanobacterium thermoformicicum SF-4. J. Biochem. (Tokyo), 114, 389-392 (1993).
4. Tachibana, A.: A novel prenyltransferase, farnesylgeranyl diphosphate synthase, from the haloalkaliphilic archaeon, Natronobacterium pharaonis. FEBS Lett., 341, 291-294 (1994).
5. Goldstein, J. L. and Brown, M. S.: Regulation of the mevalonate pathway. Nature, 343, 425-430 (1990).
6. Brown, M. S. and Goldstein, J. L.: Multivalent feedback regulation of HMG-CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth. J. Lipid Res., 21, 505-517 (1980).
7. Rohmer, M., Knani, M., Simonin, P., Sutter, B., and Sahm, H.: Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate. Biochem. J., 295, 517-524 (1993).
8. Bach, T. J.: Some new aspects of isoprenoid biosynthesis in plants. Lipids, 30, 191-202 (1995).
9. Cabrera, J. A., Bolds, J., Sields, P. E., Havel, C. M., and Watson, J. A.: Isoprenoid synthesis in Halobacterium halobium. Modulation of 3-hydroxy-3-methylglutaryl coenzyme A concentration in response to mevalonate availability. J. Biol. Chem., 261, 3578-3583 (1986).
10. Lam, W. L. and Doolittle, W. F.: Mevinoline-resistant mutations identify a promoter and the gene for a eukaryote-like 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the archaebacterium Haloferax volcanii. J. Biol. Chem., 267, 5829-5834 (1992).
11. Bult, C. J., White, O., Olsen, G. J., Zhou, L., Fleischman, R.D., Sutton, G.G., Blake, J. A., FitzGerald, L.M., Clayton, R. A., Gpcayne, J. D., Kerlavage, A. R., Dougherty, B. A., Tomb, J.-F., Adams, M. D., Reich, C. I., Overbeek, R., Kirkness, E. F., Weidman, J. F., Fuhrmann, J. L., Nguyen, D., Utterback, T. R., Kelley, J. M., Peterson, J. D., Sadow, P. W., Hnna, M. C., Cotton, M. D., Roberts, K. M., Hurst, M. A., Kaine, B. P., Borodovsky, M., Klenk, H.-P., Fraser, C. M., Smith, H. O., Woese, C. R., and Venter, J. C.: Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science, 273, 1058-1073 (1996).
12. Ekiel, I., Sprott, G. D., and Smith, I. C. P.: Mevalonic acid is partially synthesized from amino acids in Halobacterium cutribrum: a 13C nuclear magnetic resonance study. J. Bacteriol., 166, 559-564 (1986).
13. II/quinone-stimulated double condensation reaction. Biochim. Biophys. Acta, 1211, 85-96 (1994).
14. Corkey , B. E., Brandt, M., Williams, R. J., and Williamson, J. R.: Assay of short-chain acyl coenzyme A intermediates in tissue extracts by high-pressure liquid chromatography. Anal. Biochem., 118, 30-41 (1981).
15. Kikuchi, M., Narisawa, K., Tada, K., and Sweetman, L.: Enzymatic diagnosis of 3-hydroxy-3-methylglutaryl-CoA lyase deficiency with high-performance liquid chromatography. Clin. Chim. Acta, 189, 297-302 (1990).
16. DeBuysere, M. S. and Olson, M. S.: The analysis of acyl-coenzyme A derivatives by reverse-phase high-performance liquid chromatography. Anal. Biochem., 133, 373-379 (1983).
17. McCaskill, D. and Croteau, R.: Procedure for the isolation and quantification of the intermediates of the mevalonic acid pathway. Anal. Biochem., 215, 142-149 (1993).
18. Scharnagl, H., März, W., Schliack, Löser, R., and Gross, W.: A novel assay for cytosolic 3-hydroxy-3-methylglutaryl-coenzyme A synthase activity using reversed-phase ion-pair chromatography: demonstration that Lifibrol (K12.148) modulates the enzyme activity. J. Lipid Res., 36, 622-627 (1995).
19. Adams, M. W. W.: Large-scale growth of hyperthermophiles, p. 47-49. Robb, F. T. and Place, A. R. (ed.) In Archaea. A laboratory manual. Thermophiles. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1995).
20. Bradford, M. M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 74, 248-254 (1976).
21. Tachibana, A., Tanaka, T., Taniguchi, M., and Oi, S.: Evidence for farnesol-mediated isoprenoid synthesis regulation in a halophilic archaeon, Haloferax volcanii. FEBS Lett., 379, 43-46 (1996).