Rapid isolation of HCV RNA from catrimox-lysed whole blood using QIAamp® spin columns

BioTechniques

Volumn 25, Issue 6, 1998, Pages 975-978

  Ali, S A ^a,b,d   Kubik, B ^a   Gulle, H ^a   Eibl, M M ^a   Steinkasserer, A ^a,c  

^a Biomedizinische Forschungsgesellschaft mbH   (Austria)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^d UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CATION; CATRIMOX 14; GUANIDINE DERIVATIVE; ISOTHIOCYANIC ACID DERIVATIVE; RIBONUCLEASE; SURFACTANT; UNCLASSIFIED DRUG; VIRUS RNA;

ARTICLE; CYTOLYSIS; HEPATITIS C VIRUS; NONHUMAN; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PURIFICATION; VIRUS DETECTION;

ANIMALS; DNA PRIMERS; FECES; HEMOLYSIS; HEPACIVIRUS; HEPATITIS C; HUMANS; QUATERNARY AMMONIUM COMPOUNDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SURFACE-ACTIVE AGENTS; TRIMETHYL AMMONIUM COMPOUNDS;

HEPATITIS C VIRUS;

EID: 0032414742     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acidguanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
2. Hamel, A.L., M.D. Wasylyshen and G.P. Nayar. 1995. Rapid detection of bovine viral diarrhea virus by using RNA extracted directly from assorted specimens and a one-tube reverse transcription PCR assay. J. Clin. Microbiol. 33:287-291.
3. Macfarlane, D.E. and C.E. Dahle. 1993. Isolating RNA from whole blood - the dawn of RNA-based diagnosis? Nature 362:186-188.
4. Macfarlane, D.E. and C.E. Dahle. 1997. Isolating RNA from clinical samples with Catrimox-14 and lithium chloride. J. Clin. Lab. Anal. 11:132-139.
5. Mohan, M. 1996. RNA isolation from cells and tissues, p. 1-20. In P.A. Krieg (Ed.), A Laboratory Guide to RNA: Isolation, Analysis and Synthesis. John Wiley & Sons, New York.
6. Schmidt, W.N., D. Klinzman, D.R. LaBrecque, D.E. Macfarlane and J.T. Stapleton. 1995. Direct detection of hepatitis C virus (HCV) RNA from whole blood, and comparison with HCV RNA in plasma and peripheral blood mononuclear cells. J. Med. Virol. 47:153-160.
7. Schmidt, W.N., P. Wu, J. Cederna, F.A. Mitros, D.R. LaBrecque and J.T. Stapleton. 1997. Surreptitious hepatitis C virus (HCV) infection detected in the majority of patients with cryptogenic chronic hepatitis and negative HCV antibody tests. J. Infect. Dis. 176:27-33.
8. Schmidt, W.N., P. Wu, J.Q. Han, M.J. Perino, D.R. LaBrecque and J.T. Stapleton. 1997. Distribution of hepatitis C virus (HCV) RNA in whole blood and blood cell fractions: plasma HCV RNA analysis underestimates circulating virus load. J. Infect. Dis. 176:20-26.
9. Uwatoko, K., M. Sunairi, A. Yamamoto, M. Nakajima and K. Yamaura. 1996. Rapid and efficient method to eliminate substances inhibitory to the polymerase chain reaction from animal fecal samples. Vet. Microbiol. 52:73-79.