Characterization of cosmids and telomeres in cytogenetic preparations by 3D confocal fluorescence

Analytical and Quantitative Cytology and Histology

Volumn 20, Issue 6, 1998, Pages 477-482

  Kahn, Edmond ^a   Philippe, Colette ^b   Frouin, Frédérique ^a   Di Paola, Robert ^a   Bernheim, Alain ^b  

^a PITIÉ SALPÊTRIÈRE HOSPITAL   (France)

^b CNRS   (France)

Author keywords

Chromosomes; Computer assisted image processing; Confocal microscopy; Cosmids; In situ hybridization, fluorescence; Telomere

Indexed keywords

ARTICLE; COMPUTER ANALYSIS; CONFOCAL MICROSCOPY; COSMID; FLUORESCENCE MICROSCOPE; HUMAN; HUMAN CELL; IMAGE ANALYSIS; LYMPHOCYTE; MALE; NORMAL HUMAN; PRIORITY JOURNAL; TELOMERE; THREE DIMENSIONAL IMAGING;

COSMIDS; FACTOR ANALYSIS, STATISTICAL; FLUORESCENT DYES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IN SITU HYBRIDIZATION, FLUORESCENCE; INTERPHASE; MALE; MICROSCOPY, CONFOCAL; MITOSIS; TELOMERE;

EID: 0032410592     PISSN: 08846812     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Bauman J, Bayer J, Van Dekken H: Fluorescent in situ hybridization to detect cellular RNA by flow cytometry and confocal microscopy. J Microsc 1990;157:73-81
2. Benzecri JP: L'analyse des données. Second volume: L'analyse des correspondances. Paris, Dunod, 1973
3. Di Paola R, Bazin JP, Aubry F, Aurengo A, Cavaillolès F, Herry JH, Kahn E: Handling of dynamic sequences in nuclear medicine. IEEE Trans Nucl Sci 1982;29:1310-1321
4. Frouin F, Cinotti L, Benali H, Buvat I, Bazin JP, Millet P, Di Paola RR: Extraction of functional volumes from medical dynamic volumetric datasets. Comput Med Imaging Graphics 1993;17:397-404
5. Harman HH: Modern Factor Analysis. Chicago, University of Chicago Press, 1960
6. Höfers C, Baumann P, Hummers G, Jovin T, Arndt-Jovin D: The localization of chromosome domains in human interphase nuclei: Three-dimensional distance determinations of fluorescence in situ hybridization signals from confocal laser scanning microscopy. Bioimaging 1993;1:96-106
7. Johnson G, Nogueira A: A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Meth 1981;43:349-350
8. Kahn E, Frouin F, Hotmar J, Di Paola R, Bernheim A: Confocal fluorescence analysis of the depth and focus of cytogenetic preparations. Analyt Quant Cytol Histol 1997;19:404-412
9. Kahn E, Hotmar J, Bernheim A: FISH 3D chromosomal studies in nuclei from cytogenetic preparations by confocal microscopy. Analyt Quant Cytol Histol 1996;18:109-114
10. Nederlof PM, Robinson D, Abuknesha R, Wiegant J, Hopman AHN, Tanke HJ, Raap AK: Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences. Cytometry 1989;10:20-27
11. Rigaut JP, Vassy J: High-resolution three-dimensional images from confocal scanning laser microscopy: Quantitative study and mathematical correction of the effects from bleaching and fluorescence attenuation in depth. Analyt Quant Cytol Histol 1991;13:223-232
12. Rigaut JP, Vassy J, Herlin P, Duigou F, Masson E, Briane D, Foucrier J, Carjaval-Gonzalez S, Downs AM, Mandard AM: Three-dimensional DNA image cytometry by confocal scanning laser microscopy in thick tissue blocks. Cytometry 1991;12:511-524
13. Shotton DM: Electronic Light Microscopy: Techniques in Modern Biomedical Microscopy. New York, Wiley-Liss Inc, 1993
14. Slim R, Weissenbach J, Nguyen VC, Danglot G, Bernheim A: A relative order determination of four Yp cosmids on metaphase and interphase chromosomes by two-color competitive in situ hybridization. Hum Genet 1991;88:21-26
15. Van Dekken H, Van Rotterdam A, Jonker R, Van der Voort HTM, Brakenhoff G, Bauman J: Confocal microscopy as a tool for the study of the intranuclear topography of chromosomes. J Microsc 1990;158:207-214