Heptane and isooctane as embedding fluids for high-pressure freezing of Petunia ovules followed by freeze-substitution

Journal of Microscopy

Volumn 192, Issue 3, 1998, Pages 228-235

  Thijssen, M H ^a   Van Went, J L ^a   Van Aelst, A C ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

1 hexadecene; Cryofixation; Freeze substitution; Heptane; High pressure freezing; Isooctane; Ovule; Petunia x hybrida

Indexed keywords

ACETONE; FREEZING; HEPTANE; HISTOLOGY; PARAFFINS; PHASE SEPARATION; POLYOLS; TISSUE; TISSUE ENGINEERING;

1-HEXADECENE; CRYOFIXATION; EMBEDDINGS; FIXATION METHODS; FREEZE SUBSTITUTION; HIGH-PRESSURE FREEZING; ICE CRYSTALS; ISOOCTANE; OVULE; PETUNIA X HYBRIDA;

AIR;

HEPTANE; OCTANE;

ARTICLE; CELL ULTRASTRUCTURE; CELL VACUOLE; CRYOPRESERVATION; EMBEDDING; FREEZING; MELTING POINT; NONHUMAN; PHASE SEPARATION; PLANT; PRIORITY JOURNAL; TECHNIQUE;

PETUNIA; PETUNIA X HYBRIDA;

EID: 0032410427     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1998.00385.x     Document Type: Article

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