Gene expression in rod shaped cardiac myocytes, sorted by flow cytometry

Cardiovascular Research

Volumn 40, Issue 3, 1998, Pages 530-537

  Diez, Claudius ^a   Simm, Andreas ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Cardiac myocytes; Cell sorting; Gene expression; Northern blotting; Rat; Single cell RT PCR

Indexed keywords

ARTICLE; CELL HETEROGENEITY; CELL ISOLATION; CELL POPULATION; CELL SELECTION; FLOW CYTOMETRY; GENE EXPRESSION; HEART MUSCLE CELL; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN DETERMINATION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; BLOTTING, NORTHERN; CELL SEPARATION; CELL SIZE; DNA PRIMERS; FLOW CYTOMETRY; GENE EXPRESSION; IMMUNOHISTOCHEMISTRY; MALE; MYOCARDIUM; RATS; RATS, WISTAR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 0032403751     PISSN: 00086363     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0008-6363(98)00189-8     Document Type: Article

References (26)

1. Allen M.D., King C., MacDonald T.O., Himes V. VCAM-1 and E-selectin expression during cytomegalovirus infection in post-transplant myocardial biopsies. Clin Transplant. 10:1996;528-537.
2. Andreoletti L., Hober D., Decoene C.et al. Detection of enteroviral RNA by polymerase chain reaction in endomyocardial tissue of patients with chronic cardiac diseases. J Med Virol. 48:1996;53-59.
3. Becker I., Becker K.F., Rohrl M.H.et al. Single-cell mutation analysis of tumors from stained histologic slides. Lab Invest. 75:1996;801-807.
4. Booz G.W., Baker K.M. Molecular signalling mechanisms controlling growth and function of cardiac fibroblasts. Cardiovasc Res. 30:1995;537-543.
5. Brady G., Billia F., Knox J.et al. Analysis of gene expression in a complex differentiation hierarchy by global amplification of cDNA from single cells. Curr Biol. 5:1995;909-922.
6. Brilla C.G., Maisch B. Regulation of the structural remodelling of the myocardium: from hypertrophy to heart failure. Eur Heart J. 15:1994;45-52.
7. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162:1987;156-159.
8. Embleton M.J., Gorochov G., Jones P.T., Winter G. In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells. Nucleic Acids Res. 20:1992;3831-3837.
9. Engelhardt S., Bohm M., Erdmann E., Lohse M.J. Analysis of beta-adrenergic receptor mRNA levels in human ventricular biopsy specimens by quantitative polymerase chain reactions: progressive reduction of beta 1-adrenergic receptor mRNA in heart failure. J Am Coll Cardiol. 27:1996;146-154.
10. Esser C., Gottlinger C., Kremer J.et al. Isolation of full-size mRNA from ethanol-fixed cells after cellular immunofluorescence staining and fluorescence-activated cell sorting (FACS). Cytometry. 21:1995;382-386.
11. Gaynor E.M., Mirsky M.L., Lewin H.A. Use of flow cytometry and RT-PCR for detecting gene expression by single cells. Biotechniques. 21:1996;286-291.
12. Harbeck M.C., Rothenberg P.L. A technique for isolating single cells for analysis by reverse transcription polymerase chain reaction. Anal Biochem. 230:1995;193-196.
13. Jacobson S.L., Piper H.M. Cell cultures of adult cardiomyocytes as models of the myocardium. J Mol Cell Cardiol. 18:1986;661-678.
14. Kang M.J., Koh G.Y. Differential and dramatic changes of cyclin-dependent kinase activities in cardiomyocytes during the neonatal period. J Mol Cell Cardiol. 29:1997;1767-1777.
15. 2+ transients. FEBS Lett. 376:1995;24-30.
16. Moore G.D., Ayabe T., Kopf G.S., Schultz R.M. Temporal patterns of gene expression of G1-S cyclins and cdks during the first and second mitotic cell cycles in mouse embryos. Mol Reprod Dev. 45:1996;264-275.
17. Noguchi E., Sekiguchi T., Yamashita K., Nishimoto T. Molecular cloning and identification of two types of hamster cyclin-dependent kinases: cdk2 and cdk2L. Biochem Biophys Res Commun. 197:1993;1524-1529.
18. Regitz-Zagrosek V., Fielitz J., Hummel M.et al. Decreased expression of ventricular angiotensin receptor type 1 mRNA after human heart transplantation. J Mol Med. 74:1996;777-782.
19. Robbins J., Gulick J., Sanchez A., Howles P., Doetschman T. Mouse embryonic stem cells express the cardiac myosin heavy chain genes during development in vitro. J Biol Chem. 265:1990;11905-11909.
20. Schriever F., Freeman G., Nadler L.M. Follicular dendritic cells contain a unique gene repertoire demonstrated by single-cell polymerase chain reaction. Blood. 77:1991;787-791.
21. Schultheiss T., Lin Z., Lu M.H. Differential distribution of subsets of myofibrillar proteins in cardiac nonstriated and striated myofibrils. J Cell Biol. 110:1990;1159-1172.
22. Simm A., Nestler M., Hoppe V. PDGF-AA, a potent mitogen for cardiac fibroblasts from adult rats. J Mol Cell Cardiol. 29:1997;357-368.
23. Toellner K.M., Scheel-Toellner D., Seitzer U.et al. The use of reverse transcription polymerase chain reaction to analyse large numbers of mRNA species from a single cell. J Immunol Methods. 191:1996;71-75.
24. Trümper L.H., Brady G., Vicini S.et al. Gene expression in single Reed Sternberg cells of Hodgkin's disease: results from PCR generated single cell cDNA libraries. Ann Oncol. 3:1992;25-26.
25. Trümper L.H., Brady G., Bagg A.et al. Single-cell analysis of Hodgkin and Reed-Sternberg cells: molecular heterogeneity of gene expression and p53 mutations. Blood. 81:1993;3097-3115.
26. Wong H., Anderson W.D., Cheng T., Riabowol K.T. Monitoring mRNA expression by polymerase chain reaction: the 'rimer-dropping' method. Anal Biochem. 223:1994;251-258.