The deoxyribonuclease activity attributed to ribosome-inactivating proteins is due to contamination

European Journal of Biochemistry

Volumn 258, Issue 2, 1998, Pages 540-545

  Day, Philip J ^a   Lord, J Michael ^a   Roberts, Lynne M ^a  

^a UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

Deoxyribonuclease; Ribosome inactivating protein; RNA N glycosidase

Indexed keywords

DEOXYRIBONUCLEASE; GLYCOSIDASE; NUCLEASE; POKEWEED ANTIVIRUS PROTEIN; PROTEIN; RIBOSOME INACTIVATING PROTEIN; RIBOSOME RNA; RICIN;

ARTICLE; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS INHIBITION; RIBOSOME; SACCHAROMYCES CEREVISIAE;

ESCHERICHIA COLI; PHYTOLACCACEAE; SACCHAROMYCES CEREVISIAE;

EID: 0032401544     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2580540.x     Document Type: Article

References (40)

1. Endo, Y. & Tsurugi, K. (1987) RNA N-glycosidase activity of ricin A chain - mechanism of action of the toxic lectin on eukarvotic ribosomes, J. Biol. Chem. 262, 8128-8130.
2. Rapak, A., Falnes, P. O. & Olsnes, S. (1997) Retrograde transport of mutant ricin to the endoplasmic reticulum with subsequent translocation to the cytosol, Proc. Natl Acad. Sci. USA 94, 3783-3788.
3. Leah, R., Tommerup, H., Svendsen, I. & Mundy, J. (1991) Biochemical and molecular characterization of three barley seed proteins with antifungal properties, J. Biol. Chem. 266, 1564-1573.
4. Barbieri, L., Gorini, P., Valbonesi, P., Castiglioni, P. & Stirpe. F. (1994) Unexpected activity of saporins, Nature 372, 624.
5. Barbieri, L., Valbonesi, P., Bonora, E., Gorini, P., Bolognesi, A. & Stirpe, F. (1997) Polynucleotide:adenosine glycosidase activity of ribsome-inactivating proteins: effect on DNA. RNA and poly A, Nucleic Acids Res. 25, 519-522.
6. Olivieri, F., Prasad, V., Valbonesi, P., Srivastava, S., Ghosal-Chowdhury, P., Barbieri, L., Bolognesi, A. & Stirpe, F. (1996) A systemic antiviral resistance-inducing protein isolated from Clerodendrum inerme gaertn is a polynucleotide-adenosine glycosidase (ribosome inactivating protein), FEBS Lett. 396, 132-134.
7. Chen, H., Hua, L., Wang, Y., Zhao, K., Yan, M. G. & Dong, Y. C. (1996) Ribonucleuse activity of single-chain ribosome-inactivating proteins, Prog. Biochem. Biophys. 23, 435-456.
8. Ling, J., Li, X.-d., Wu, X.-h. & Liu, W.-y. (1995) Topological requirements for recognition and cleavage of DNA by ribosomeinactivating proteins, Biol. Chem. Hoppe-Seyler 376, 637-641.
9. Li, M-X., Yeung, H.-W., Pan, L.-P. & Chan, S. I. (1991) Trichosanthin, a potent HIV-1 inhibitor, can cleave supercoiled DNA in vitro, Nucleic Acids Res. 19, 6309-6312.
10. Nicolas, N., Beggs, J. M., Haltiwanger, B. M. & Taraschi, T. F. (1997) Direct evidence for the deoxyribonuclease activity of the plant ribosome inactivating protein gelonin. FEBS Lett. 406, 162-164.
11. Roncuzzi, L. & Gasperi-Campani, A. (1996) DNA-nuclease activity of the single-chain ribosome-inactivating proteins dianthin-30, saporin-6 and gelonin. FEBS Lett. 392, 16-20.
12. Lee-Huang, S., Kung, H.-f., Huang, P. L., Bourinbaiar, A., Morell, J. L., Brown, J. H., Huang, P. L., Tsai, W.-P., Chen, A. Y., Huang, H. I. & Chen, H.-C. (1994) Human immunodeficiency virus type1 (HIV-1) inhibition, DNA-binding, RNA-binding, and ribosome inactivation activities in the N-terminal segments of the plant antiHIV protein GAP31, Proc. Natl Acad. Sci. USA 91, 12208-12212.
13. Lee-Huang, S., Huang, P. L., Chen, H.-C., Huang, P. L., Bourinbaiar, A., Huang, H. I. & Kung, H.-f. (1995) Anti-HIV and antitumor activities of recombinant MAP30 from bitter melon, Gene (Amst.) 161, 151-156.
14. Li, X.-d., Chen, W.-f., Liu, W.-y. & Wang, G.-h. (1997) Large-scale preparation of two new ribosome-inactivating proteins-cinnamomin and camphorin from the seeds of Cinnamomum camphora, Protein Expr. Purif. 10, 27-31.
15. Nicolas, N., Goodyer, I. D. & Taraschi, T. F. (1997) An additional mechanism of ribosome-inactivating protein cytotoxicity: degradation of extrachromosomal DNA, Biochem. J. 327, 413-417.
16. Chang, M. P., Baldwin, R. L., Bruce, C. & Wisnieski, B. J. (1989) Second cytotoxic pathway of diphtheria toxin suggested by nuclease activity, Science 246, 1165-1168.
17. Lessnick, S. L., Bruce, C., Baldwin, R. L., Chang, M. P., Nakamura, L. T. & Wisnieski, B. J. (1990) Does diphtheria toxin have nuclease activity - reply, Science 250, 836-838.
18. Johnson, V. G. (1990) Does diphtheria toxin have nuclease activity? Science 250, 832-834.
19. Wilson, B. A., Blanke, S. R., Murphy, J. R., Pappenheimer, A. M. Jr & Collier, R. J. (1990) Does diphtheria toxin have nuclease activity? Science 250, 834-835.
20. Ling, J., Liu, W.-y. & Wang, T. P. (1994) Cleavage of supercoiled double stranded DNA by several ribosome-inactivating proteins in vitro, FEBS Lett. 345, 143-146.
21. Rothblatt, J. A. & Meyer, D. I. (1986) Secretion in yeast - reconstitution of the translocation and glycosylation of alpha factor and invertase in a homologous cell free system. Cell 44, 619-628.
22. Rheinberger, H. J., Geigenmuller, H., Wedde, M. & Nierhaus, K. H. (1988) Parameters for the preparation of Eschericia coli ribosomes and ribosomal subunits active in transfer RNA binding, Methods Enzymol. 164, 658-670.
23. Endo, Y., Mitsui, K., Motizuki, M. & Tsurugi, K. (1987) The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes - the site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins, J. Biol. Chem. 262, 5908-5912.
24. Kirby, K. S. (1968) Isolution of nucleic acids with phenolic solvents, Methods Enzymol. 12b, 87-100.
25. May, M. J., Hartley, M. R., Roberts, L. M., Krieg, P A., Osborn, R. W. & Lord, J. M. (1989) Ribosome inactivation by ricin A chain: a sensitive method to assess the activity of wild type and mutant polypeptides, EMBO J. 8, 301-308.
26. Chaddock, J. A., Monzingo, A. F., Robertus, J. D., Lord, J. M. & Roberts, L. M. (1996) Major structural differences between pokeweed antiviral protein and ricin A chain do not account for their differing ribosome specificity, Eur. J. Biochem. 235, 159-166.
27. Bodley, J. W. (1990) Does diphtheria toxin have nuclease activity? Science 250, 832.
28. Day, P. J., Ernst, S. R., Frankel, A. E., Monzingo, A. F., Pascal, J. M., Molina-Svinth, M. C. & Robertus, J. D. (1996) Structure and activity of an active site substitution of ricin A chain. Biochemistry 35, 11098-11103.
29. Legname, G., Bellosta, P., Gromo, G., Modena, D., Keen, J. N., Roberts, L. M. & Lord, J. M. (1991) Nucleotide sequence of cDNA coding for dianthin 30, a ribosome inactivating protein from Dianthus caryophyllus, Biochim. Biophys. Acta 1090, 119-122.
30. Asano, K. & Svensson, B. (1986) Chemical modification studies on protein synthesis inhibitor II from barley seeds - identification of an essential tyrosyl residue, Carlsherg Res. Commun. 51, 501-507.
31. Ready, M. P., Kim, Y. & Robertus, J. D. (1991) Site directed mutagenesis of ricin A chain and implications for the mechanism of action, Proteins: Struct. Funct. Genet. 10, 270-278.
32. Fukaya, N. & Taniguchi, T. (1979) Some properties of an inhibitor of plant virus infection occurring in the leaves of Phytolacca americana, Phytopathol. Z. 94, 132-138.
33. Monzingo, A. F., Collins, E. J., Ernst, S., Irvin, J. D. & Robertus, J. D. (1993) The 2.5 A structure of pokeweed antiviral protein. J. Mol. Biol. 233, 705-715.
34. Lin, Q., Chen, Z. C., Antoniw, J. F. & White, R. F. (1991) Isolation and characterization of a cDNA clone encoding the antiviral protein from Phytolacca americana. Plant Mol. Biol. 17, 609-614.
35. Chen, Z. C., Antoniw, J. F., Lin, Q. & White, R. F. (1993) Expression of pokeweed (Phytolacca americana) antiviral protein cDNA in Eschericia coli and its antiviral activity, Physiol. Mol. Plant Pathol. 42, 237-247.
36. Simpson, J. C., Lord, J. M. & Roberts, L. M. (1995) Point mutations in the hydrophobic C-terminul region of ricin A chain indicate that Pro250 plays a key role in membrasne translocation. Eur. J. Biochem. 232, 458-463.
37. Wales, R., Roberts, L. M. & Lord, J. M. (1993) Addition of an endoplasmic reticulum retrieval sequence to ricin A chain significantly increases its cytotoxicity to mammalain cells. J. Biol. Chem. 268, 23987-23990.
38. Tumer, N. E., Hwang, D.-J. & Bonness, M (1997) A C-terminal deletion mutant of pokeweed antiviral protein inhibits viral infection but does not depurinate host ribosomes. Proc. Natl Acad. Sci. USA 94, 3866-3871.
39. Taylor, S., Massiah, A., Lomonossoff, G., Roberts, L. M., Lord, J. M. & Hartley, M. R. (1994) Correlation between the activities of five ribosome inactivating proteins in the depurination of tobacco ribosomes and inhibition of tobacco mosaic virus infection. Plant J. 5, 827-835.
40. Frankel, A., Welsh, P., Richardson, J. & Robertus, J. D. (1990) Role of arginine 180 and glutamic acid 177 of ricin toxin A chain in enzymatic inactivation of ribosomes. Mol. Cell Biol. 10, 6257-6263.