Purification and characterization of two distinct carboxymethylcellulases of Paecilomyces sp.

Bioresource Technology

Volumn 66, Issue 3, 1998, Pages 231-234

  Okolo, J C ^a,c   Obi, S K C ^a   Odibo, F J C ^b  

^a UNIVERSITY OF NIGERIA   (Nigeria)

^b NNAMDI AZIKIWE UNIVERSITY   (Nigeria)

^c FEDERAL UNIVERSITY OF TECHNOLOGY   (Nigeria)

Author keywords

Carboxymethylcellulase; Purification; Rice hull

Indexed keywords

ACETONE; CATALYST ACTIVITY; CELL CULTURE; CHROMATOGRAPHY; ENZYME INHIBITION; FILTRATION; HULLS (SEED COVERINGS); ION EXCHANGE; MOLECULAR WEIGHT; PH EFFECTS; PRECIPITATION (CHEMICAL); PURIFICATION;

CARBOXYMETHYLCELLULASE;

ENZYME KINETICS;

CARBOXYMETHYLCELLULASE; CELLULASE; UNCLASSIFIED DRUG;

ARTICLE; BIODEGRADATION; BIOTRANSFORMATION; GEL FILTRATION; ION EXCHANGE CHROMATOGRAPHY; PH; PRIORITY JOURNAL; PURIFICATION; RICE;

PAECILOMYCES;

EID: 0032387992     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-8524(98)00053-4     Document Type: Article

References (9)

1. Andrews, P. (1964). Estimation of the molecular weight of proteins by Sephadex gel filtration. Biochem. J., 91, 222-233.
2. Bernfeld, P. (1955). Amylases α/β. Methods Enzymol., 1, 149-158.
3. Gosh, B. K. & Gosh, A. (1992). Degradation of cellulose by fungal cellulase. In Microbial Degradation of Natural Products, ed. G. Winkelmann, pp. 83-159, VCH, Weinheim.
4. Horikoshi, K., Nakao, M., Kurono, Y. & Sashihara, M. (1984). Cellulases of an alkalophilic Bacillus strain isolated from soil. Can. J. Microbiol., 30, 774-779.
5. Lidner, W. (1988). Carboxymethylcellulase from Sclerotium rolfsii. Methods Enzymol., 160, 376-382.
6. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265-275.
7. Oguntimein, G., Vlach, D. & Moo-Young, M. (1992). Production of cellulolytic enzymes by Neurospora sitophila grown on cellulosic materials. Bioresource Technol., 39, 277-283.
8. Sinitsyn, A. P., Gusakov, A. V., Davydkin, I. Y., Davydkin, V. Y. & Protas, O. V. (1993). A hyper-efficient process for enzymatic cellulose hydrolysis in the intensive mass transfer reactor. Biotechnol. Lett., 15, 283-288.
9. Tong, C., Cole, A. & Shepherd, G. (1980). Purification and properties of the cellulase from the thermophilic fungus, Thermoascus aurantiacus. Biochem. J., 191, 83-94.